Construct: sgRNA BRDN0001149265
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAGAACTCAAATTTACCCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EPHA5 (2044)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76677
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000004.12 |
4 |
65602152 |
- |
EPHA5 |
NNNAACTCAAATTTACCCTG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000001.11 |
1 |
117090547 |
- |
TTF2 |
NNNAACTGAAATTTACGCTG |
NGG |
2 |
0.0378 |
Tier I |
3 |
NC_000015.10 |
15 |
53534486 |
+ |
WDR72 |
NNNAACTTAAATTTACCCTA |
NGG |
2 |
0.8203 |
Tier II |
4 |
NC_000012.12 |
12 |
38694097 |
- |
CPNE8 |
NNNAACTCAAAAATACCCTG |
NGG |
2 |
0.5538 |
Tier II |
5 |
NC_000012.12 |
12 |
86673286 |
- |
MGAT4C |
NNNAACTCAAAGTTACCCTA |
NGG |
2 |
0.4688 |
Tier II |
6 |
NC_000003.12 |
3 |
197883398 |
+ |
LRCH3 |
NNNAACTCATATTTACACTG |
NGG |
2 |
0.4118 |
Tier II |
7 |
NC_000009.12 |
9 |
99826292 |
- |
NR4A3 |
NNNAACTCAAATTTACTCTT |
NGG |
2 |
0.3267 |
Tier II |
8 |
NC_000006.12 |
6 |
149730568 |
+ |
NUP43 |
NNNTACTCAAATTTACTCTG |
NGG |
2 |
0.297 |
Tier II |
9 |
NC_000011.10 |
11 |
17195084 |
+ |
PIK3C2A |
NNNAACTTAATTTTACCCTG |
NGG |
2 |
0.2692 |
Tier II |
10 |
NC_000016.10 |
16 |
70953748 |
+ |
HYDIN |
NNNAACTCAAATTCACCCTT |
NGG |
2 |
0.2 |
Tier II |
11 |
NC_000010.11 |
10 |
1337658 |
+ |
ADARB2 |
NNNACCTCAACTTTACCCTG |
NGG |
2 |
0.2 |
Tier II |
12 |
NC_000004.12 |
4 |
138181780 |
+ |
SLC7A11 |
NNNAACTCAAATTTACCTCG |
NGG |
2 |
0.1773 |
Tier II |
13 |
NC_000011.10 |
11 |
132868919 |
+ |
OPCML |
NNNATCTCAAATTTACCCTC |
NGG |
2 |
0.1558 |
Tier II |
14 |
NC_000004.12 |
4 |
120872307 |
+ |
PRDM5 |
NNNAACTCAAATTTCCACTG |
NGG |
2 |
0.1273 |
Tier II |
15 |
NC_000005.10 |
5 |
120527545 |
- |
PRR16 |
NNNAACTCAAAATTAGCCTG |
NGG |
2 |
0.1231 |
Tier II |
16 |
NC_000003.12 |
3 |
60122369 |
- |
FHIT |
NNNAACTCAAATGTTCCCTG |
NGG |
2 |
0.0522 |
Tier II |
17 |
NC_000024.10 |
Y |
13362248 |
- |
UTY |
NNNAACTCAAATATACCCTG |
NGC |
2 |
0.0154 |
Tier II |
18 |
NC_000014.9 |
14 |
96368513 |
+ |
GSKIP |
NNNAACTCAAATTAACCCTG |
NGT |
2 |
0.01 |
Tier II |
19 |
NC_000015.10 |
15 |
53534486 |
+ |
LOC105370826 |
NNNAACTTAAATTTACCCTA |
NGG |
2 |
0.8203 |
Tier III |
20 |
NC_000007.14 |
7 |
10104754 |
- |
LOC105375149 |
NNNAAATGAAATTTACCCTG |
NGG |
2 |
0.5969 |
Tier III |
21 |
NC_000014.9 |
14 |
95397030 |
- |
LOC105370640 |
NNNAATTCAAATTTACCCTG |
NAG |
2 |
0.2407 |
Tier III |
22 |
NC_000001.11 |
1 |
146763717 |
- |
HYDIN2 |
NNNAACTCAAATTCACCCTT |
NGG |
2 |
0.2 |
Tier III |
23 |
NC_000001.11 |
1 |
31563425 |
- |
LOC124903917 |
NNNAACTCACATTTACTCTG |
NGG |
2 |
0.1556 |
Tier III |
24 |
NC_000006.12 |
6 |
146903544 |
+ |
STXBP5-AS1 |
NNNAACTCAAATTGACCGTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000076.6 |
10 |
110045867 |
- |
Nav3 |
NNNAACTAAAACTTACCCTG |
NGG |
2 |
0.6158 |
Tier II |
2 |
NC_000070.6 |
4 |
43580100 |
- |
Rgp1 |
NNNAACTTAACTTTACCCTG |
NGG |
2 |
0.35 |
Tier II |
3 |
NC_000076.6 |
10 |
8436621 |
+ |
Ust |
NNNAACTCCAATTTACTCTG |
NGG |
2 |
0.2667 |
Tier II |
4 |
NC_000074.6 |
8 |
61900746 |
+ |
Palld |
NNNATCTCAAGTTTACCCTG |
NGG |
2 |
0.2364 |
Tier II |
5 |
NC_000079.6 |
13 |
44978091 |
+ |
Dtnbp1 |
NNNAACTCAAATTCACCCTT |
NGG |
2 |
0.2 |
Tier II |
6 |
NC_000077.6 |
11 |
80713027 |
- |
Myo1d |
NNNAACTAAAATTTACCCTG |
NAG |
2 |
0.1685 |
Tier II |
7 |
NC_000068.7 |
2 |
6030361 |
- |
Upf2 |
NNNAACTCAGATTTACCCTG |
NAG |
2 |
0.144 |
Tier II |
8 |
NC_000081.6 |
15 |
101710512 |
- |
Krt5 |
NNNAACCCAAATTTTCCCTG |
NGG |
2 |
0.1375 |
Tier II |
9 |
NC_000073.6 |
7 |
84064921 |
- |
Cemip |
NNNAACTCAAATTTAGCCTC |
NGG |
2 |
0.0659 |
Tier II |
10 |
NC_000074.6 |
8 |
14742737 |
- |
Dlgap2 |
NNNAACTCAAATTTTTCCTG |
NGG |
2 |
0.0615 |
Tier II |
11 |
NC_000079.6 |
13 |
93380762 |
+ |
Homer1 |
NNNAACTCAGATTTACCCTG |
NGA |
2 |
0.0386 |
Tier II |
12 |
NC_000086.7 |
X |
56559299 |
- |
Gm648 |
NNNAACTCAAATTTTGCCTG |
NGG |
2 |
0.0308 |
Tier II |
13 |
NC_000069.6 |
3 |
90345480 |
+ |
Gatad2b |
NNNAACTCAGATTTACCCTG |
NTG |
2 |
0.0216 |
Tier II |
14 |
NC_000069.6 |
3 |
141769217 |
- |
Unc5c |
NNNAACTCAAATTTACCTTG |
NGT |
2 |
0.0104 |
Tier II |
15 |
NC_000084.6 |
18 |
24281927 |
- |
Galnt1 |
NNNAACTCAAATTTTCCCTG |
NGC |
2 |
0.0044 |
Tier II |
16 |
NC_000075.6 |
9 |
57104930 |
- |
Sin3a |
NNNAACTGAAATTGACCCTG |
NGG |
2 |
0.0 |
Tier II |
17 |
NC_000078.6 |
12 |
6087487 |
+ |
Gm32576 |
NNNAACACAAAATTACCCTG |
NGG |
2 |
0.7 |
Tier III |
Other clones with same target sequence:
(none)