GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001149266

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: TGTTGAGGGGAGCCTCACGT

Original Target:

Taxon:: Homo sapiens (human)
Gene:: AKT1 (207)
Gene Description:: n/a
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 75501

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000014.9	14	104780120	+	AKT1	NNNTGAGGGGAGCCTCACGT	NGG	0	1.0	Tier I
2	NC_000014.9	14	104949387	+	AHNAK2	NNNGGAGGGGAGACTCACGT	NGG	2	0.2404	Tier I
3	NC_000014.9	14	104951862	+	AHNAK2	NNNGGAGGGGAGACTCACGT	NGG	2	0.2404	Tier I
4	NC_000014.9	14	104942952	+	AHNAK2	NNNGGAGGGGAGGCTCACGT	NGG	2	0.0852	Tier I
5	NC_000014.9	14	104945427	+	AHNAK2	NNNGGAGGGGAGGCTCACGT	NGG	2	0.0852	Tier I
6	NC_000014.9	14	104948397	+	AHNAK2	NNNGGAGGGGAGGCTCACGT	NGG	2	0.0852	Tier I
7	NC_000014.9	14	104950872	+	AHNAK2	NNNGGAGGGGAGGCTCACGT	NGG	2	0.0852	Tier I
8	NC_000002.12	2	1409121	+	TPO	NNNTGAGAGGAGCCTCACAT	NGG	2	0.7143	Tier II
9	NC_000012.12	12	120004084	+	BICDL1	NNNTGAGCAGAGCCTCACGT	NGG	2	0.3956	Tier II
10	NC_000011.10	11	126944656	-	KIRREL3	NNNTGCGGGGAGCCTCACTT	NGG	2	0.303	Tier II
11	NC_000016.10	16	81467398	-	CMIP	NNNTGAGGGGAGCCTCACAG	NGG	2	0.1261	Tier II
12	NC_000014.9	14	105261305	-	BRF1	NNNTGATGGGAGCCTCGCGT	NGG	2	0.1008	Tier II
13	NC_000005.10	5	16704469	-	MYO10	NNNTGAGGGGAGCCTCAGGT	NAG	2	0.0346	Tier II
14	NC_000022.11	22	22788945	+	IGL	NNNTCAGGGGAGTCTCACGT	NGG	2	0.55	Tier III
15	NC_000003.12	3	67863739	-	SUCLG2-DT	NNNTGAGGAGAGCCTCACTT	NGG	2	0.4286	Tier III
16	NC_000011.10	11	126944656	-	KIRREL3-AS2	NNNTGCGGGGAGCCTCACTT	NGG	2	0.303	Tier III
17	NC_000011.10	11	67348819	-	LOC100130987	NNNTGAGAGGAGCCTCAGGT	NGG	2	0.1333	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000080.6	14	50820017	-	Parp2	NNNTGATGGGAGCCTCAGGT	NGG	2	0.0762	Tier I
2	NC_000067.6	1	129707623	+	Thsd7b	NNNTCAGGGGAGCCTCACCT	NGG	2	0.3522	Tier II
3	NC_000068.7	2	33300312	+	Ralgps1	NNNTGAGGGGAGCCTCACCT	NAG	2	0.1162	Tier II
4	NC_000080.6	14	70001491	-	Pebp4	NNNTGTGGGGAGCCTCAGGT	NGG	2	0.0952	Tier II
5	NC_000082.6	16	91717695	+	Cryzl1	NNNTAAGGGGAGCCTCACGT	NTG	2	0.0338	Tier II
6	NC_000078.6	12	33385482	-	Atxn7l1os2	NNNTGAGGGGAGTCTCACAT	NGG	2	0.5	Tier III
7	NC_000080.6	14	70001491	-	Gm33524	NNNTGTGGGGAGCCTCAGGT	NGG	2	0.0952	Tier III

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Other clones with same target sequence:

(none)