Construct: sgRNA BRDN0001149268
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTGTTTCACCCATAAGCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MLKL (197259)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77541
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
74695375 |
- |
MLKL |
NNNGTTTCACCCATAAGCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
24835849 |
- |
RIPOR2 |
NNNGGTTCACCCAGAAGCCA |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000004.12 |
4 |
186279851 |
- |
F11 |
NNNGTTACACCCATAAACCA |
NGG |
2 |
0.8167 |
Tier II |
| 4 |
NC_000011.10 |
11 |
92243769 |
+ |
FAT3 |
NNNGTTTGAACCATAAGCCA |
NGG |
2 |
0.5571 |
Tier II |
| 5 |
NC_000003.12 |
3 |
139649517 |
+ |
NMNAT3 |
NNNGTTTCACACAAAAGCCA |
NGG |
2 |
0.4643 |
Tier II |
| 6 |
NC_000007.14 |
7 |
12240935 |
- |
TMEM106B |
NNNGTTTCACCCATAAACCA |
NAG |
2 |
0.242 |
Tier II |
| 7 |
NC_000007.14 |
7 |
146395890 |
+ |
CNTNAP2 |
NNNGTTTCACCTATAAGCAA |
NGG |
2 |
0.2308 |
Tier II |
| 8 |
NC_000005.10 |
5 |
113231535 |
+ |
MCC |
NNNGTTTCACCCATTAGTCA |
NGG |
2 |
0.1286 |
Tier II |
| 9 |
NC_000011.10 |
11 |
64803948 |
- |
MEN1 |
NNNGTTTCACCCTTAACCCA |
NGG |
2 |
0.0706 |
Tier II |
| 10 |
NC_000022.11 |
22 |
33851030 |
- |
LARGE1 |
NNNGTTTCACCCATAAGCCA |
NGT |
1 |
0.0161 |
Tier II |
| 11 |
NC_000010.11 |
10 |
35175257 |
- |
CREM |
NNNGTTTCACCCATTAGCCA |
NGA |
2 |
0.0139 |
Tier II |
| 12 |
NC_000018.10 |
18 |
795022 |
+ |
YES1 |
NNNGTTTCACCCATTAGCCA |
NGA |
2 |
0.0139 |
Tier II |
| 13 |
NC_000001.11 |
1 |
110671507 |
- |
KCNA3 |
NNNGTTTCAGCCATATGCCA |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NT_113888.1 |
14 |
112120 |
+ |
MAFIP |
NNNGTTTCATCCATAAGTCA |
NGG |
2 |
0.605 |
Tier III |
| 15 |
NC_000005.10 |
5 |
61211186 |
- |
SMIM15-AS1 |
NNNGTTTAACCCAAAAGCCA |
NGG |
2 |
0.4024 |
Tier III |
| 16 |
NC_000022.11 |
22 |
22599731 |
- |
IGL |
NNNGATTCACCTATAAGCCA |
NGG |
2 |
0.2692 |
Tier III |
| 17 |
NC_000008.11 |
8 |
23977466 |
- |
LOC107986931 |
NNNCTTTCACCCATAACCCA |
NGG |
2 |
0.1246 |
Tier III |
| 18 |
NC_000013.11 |
13 |
81830480 |
- |
LOC105370284 |
NNNGTTTCACCCATCAGCCA |
NAG |
2 |
0.0707 |
Tier III |
| 19 |
NC_000004.12 |
4 |
142971145 |
+ |
USP38-DT |
NNNGTTTCACACATAAGCCA |
NGA |
2 |
0.0521 |
Tier III |
| 20 |
NC_000010.11 |
10 |
35716163 |
- |
LOC107984188 |
NNNGTTTCACCCATTAGCCA |
NGA |
2 |
0.0139 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
57918830 |
+ |
Micu2 |
NNNGTTTTACCCATAAGCCT |
NGG |
2 |
0.525 |
Tier I |
| 2 |
NC_000067.6 |
1 |
90842482 |
+ |
Col6a3 |
NNNTCTTCACCCATAAGCCA |
NGG |
2 |
0.3636 |
Tier II |
| 3 |
NC_000069.6 |
3 |
139040622 |
- |
Rap1gds1 |
NNNTTTTCACCCATAAGCTA |
NGG |
2 |
0.1678 |
Tier II |
| 4 |
NC_000070.6 |
4 |
116650056 |
+ |
Akr1a1 |
NNNGTTTCCCCCACAAGCCA |
NGG |
2 |
0.1633 |
Tier II |
| 5 |
NC_000080.6 |
14 |
18016574 |
- |
Thrb |
NNNGTTTCACCCATAAGCAC |
NGG |
2 |
0.0974 |
Tier II |
| 6 |
NC_000073.6 |
7 |
91780092 |
+ |
Dlg2 |
NNNGTTTCACCCATAAGGCA |
NGT |
2 |
0.0022 |
Tier II |
| 7 |
NC_000068.7 |
2 |
79235137 |
- |
Gm35813 |
NNNGATTCACCCATCAGCCA |
NGG |
2 |
0.1364 |
Tier III |
| 8 |
NC_000068.7 |
2 |
79235137 |
- |
Gm39866 |
NNNGATTCACCCATCAGCCA |
NGG |
2 |
0.1364 |
Tier III |
Other clones with same target sequence:
(none)
