Construct: sgRNA BRDN0001149270
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTACGACGACACTGTCCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- WEE1 (7465)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77370
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
9576057 |
- |
WEE1 |
NNNACGACGACACTGTCCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
32784787 |
- |
TMEM215 |
NNNACGATGATACTGTCCTG |
NGG |
2 |
0.2692 |
Tier I |
| 3 |
NC_000001.11 |
1 |
33912069 |
- |
CSMD2 |
NNNAGGAGGACACTGTCCTG |
NGG |
2 |
0.3857 |
Tier II |
| 4 |
NC_000006.12 |
6 |
85460621 |
+ |
NT5E |
NNNATGACCACACTGTCCTG |
NGG |
2 |
0.3427 |
Tier II |
| 5 |
NC_000015.10 |
15 |
101047736 |
- |
LRRK1 |
NNNACGACGAGACTGTCCTG |
NGC |
2 |
0.0056 |
Tier II |
| 6 |
NC_000015.10 |
15 |
101047736 |
- |
LOC105371026 |
NNNACGACGAGACTGTCCTG |
NGC |
2 |
0.0056 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
40474528 |
- |
Tmem215 |
NNNACGATGATACTGTCCTG |
NGG |
2 |
0.2692 |
Tier I |
| 2 |
NC_000071.6 |
5 |
73547920 |
- |
Dcun1d4 |
NNNACAAGGACACTGTCCTG |
NGG |
2 |
0.6429 |
Tier II |
| 3 |
NC_000075.6 |
9 |
69140531 |
+ |
Rora |
NNNAAGACAACACTGTCCTG |
NGG |
2 |
0.3673 |
Tier II |
| 4 |
NC_000068.7 |
2 |
168521569 |
- |
Nfatc2 |
NNNACGAGGACACTGTCCTC |
NGG |
2 |
0.2755 |
Tier II |
Other clones with same target sequence:
(none)
