Construct: sgRNA BRDN0001149273
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTAATATCATGGCGACAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME5 (8382)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76571
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
138129361 |
+ |
NME5 |
NNNAATATCATGGCGACAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
77087594 |
+ |
MYCBP2 |
NNNAATATCATGGAGAGAAG |
NGG |
2 |
0.0206 |
Tier I |
| 3 |
NC_000006.12 |
6 |
64456536 |
+ |
EYS |
NNNAATACCATGGCTACAAG |
NGG |
2 |
0.1429 |
Tier II |
| 4 |
NC_000023.11 |
X |
151630530 |
- |
PASD1 |
NNNAATATCATGGGGACAAG |
NGA |
2 |
0.0 |
Tier II |
| 5 |
NC_000001.11 |
1 |
215937595 |
- |
USH2A |
NNNAATATCATGGGGACAGG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000001.11 |
1 |
87396768 |
+ |
LOC105378833 |
NNNAATATCATGGCGAAAAG |
NGG |
1 |
0.4667 |
Tier III |
| 7 |
NC_000010.11 |
10 |
43872115 |
- |
LINC00840 |
NNNAATATCATGGCCACATG |
NGG |
2 |
0.1469 |
Tier III |
| 8 |
NC_000006.12 |
6 |
132098435 |
- |
CCN2-AS1 |
NNNAATATCATGACTACAAG |
NGG |
2 |
0.1319 |
Tier III |
| 9 |
NC_000013.11 |
13 |
77087594 |
+ |
MYCBP2-AS1 |
NNNAATATCATGGAGAGAAG |
NGG |
2 |
0.0206 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
34571554 |
+ |
Nme5 |
NNNAATATCATAGCAACAAG |
NGG |
2 |
0.8784 |
Tier I |
| 2 |
NC_000080.6 |
14 |
13529658 |
+ |
Synpr |
NNNAATACCATGGAGACAAG |
NGG |
2 |
0.35 |
Tier II |
| 3 |
NC_000069.6 |
3 |
105659638 |
+ |
Kcnd3 |
NNNAACATCATGGCGACAGG |
NGG |
2 |
0.3409 |
Tier II |
| 4 |
NC_000080.6 |
14 |
49346998 |
+ |
Slc35f4 |
NNNAATATCATGGTGGCAAG |
NGG |
2 |
0.141 |
Tier II |
| 5 |
NC_000082.6 |
16 |
42290651 |
- |
Gap43 |
NNNAATATCATGGCAACAAG |
NGT |
2 |
0.0152 |
Tier II |
| 6 |
NC_000084.6 |
18 |
31204393 |
- |
Rit2 |
NNNAATATCATGGGGACAAT |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000070.6 |
4 |
56457940 |
- |
Gm34628 |
NNNAATGTCATGGAGACAAG |
NGG |
2 |
0.2471 |
Tier III |
Other clones with same target sequence:
(none)
