Construct: sgRNA BRDN0001149279
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTCGGGTGGCCAGTCAAAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AK8 (158067)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77882
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
132827037 |
+ |
AK8 |
NNNGGGTGGCCAGTCAAAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
226891802 |
- |
PSEN2 |
NNNGGGTAGCCAGTCAAGGG |
NGG |
2 |
0.4 |
Tier I |
| 3 |
NC_000023.11 |
X |
18967326 |
+ |
PHKA2 |
NNNGGCTGGCCAGTCACAGG |
NGG |
2 |
0.1203 |
Tier II |
| 4 |
NC_000012.12 |
12 |
41502602 |
- |
PDZRN4 |
NNNGGGTGTCCAGTAAAAGG |
NGG |
2 |
0.1185 |
Tier II |
| 5 |
NC_000019.10 |
19 |
10361481 |
+ |
TYK2 |
NNNGGGTGGCCTGCCAAAGG |
NGG |
2 |
0.0952 |
Tier II |
| 6 |
NC_000001.11 |
1 |
21225614 |
+ |
ECE1 |
NNNTGGTGGCCAGTCAGAGG |
NGG |
2 |
0.0642 |
Tier II |
| 7 |
NC_000003.12 |
3 |
120996951 |
- |
STXBP5L |
NNNGGGTGGTCAGTGAAAGG |
NGG |
2 |
0.0471 |
Tier II |
| 8 |
NC_000008.11 |
8 |
60683673 |
- |
CHD7 |
NNNGGGTGGCCAGTCAGAGG |
NAG |
2 |
0.0458 |
Tier II |
| 9 |
NC_000001.11 |
1 |
41689274 |
- |
HIVEP3 |
NNNGGGAGGCCAGTGAAAGG |
NGG |
2 |
0.0438 |
Tier II |
| 10 |
NC_000015.10 |
15 |
48447046 |
+ |
FBN1 |
NNNGGCTGGCCAGTGAAAGG |
NGG |
2 |
0.0341 |
Tier II |
| 11 |
NC_000016.10 |
16 |
88235367 |
- |
ZNF469 |
NNNGGGTGGCCACTCAAAGG |
NGA |
2 |
0.0292 |
Tier II |
| 12 |
NC_000002.12 |
2 |
216642827 |
- |
IGFBP2 |
NNNGGCTGGCCAGGCAAAGG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000015.10 |
15 |
96193653 |
- |
NR2F2-AS1 |
NNNGGGTGGCAAGTCAATGG |
NGG |
2 |
0.375 |
Tier III |
| 14 |
NC_000015.10 |
15 |
96193653 |
- |
LOC112268156 |
NNNGGGTGGCAAGTCAATGG |
NGG |
2 |
0.375 |
Tier III |
| 15 |
NC_000002.12 |
2 |
207229481 |
- |
MYOSLID-AS1 |
NNNGGGTGGCCACTCAAAGT |
NGG |
2 |
0.2947 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
16604938 |
- |
Gna14 |
NNNAGGTGGACAGTCAAAGG |
NGG |
2 |
0.78 |
Tier II |
| 2 |
NC_000072.6 |
6 |
55174356 |
- |
Inmt |
NNNGGGTGGCCAGTCAAAGT |
NAG |
2 |
0.1815 |
Tier II |
| 3 |
NC_000074.6 |
8 |
108871078 |
+ |
Zfhx3 |
NNNAGGTGGCCAGTCACAGG |
NGG |
2 |
0.1588 |
Tier II |
| 4 |
NC_000073.6 |
7 |
98944813 |
+ |
Uvrag |
NNNAGGTGGCCAGTCAGAGG |
NGG |
2 |
0.1588 |
Tier II |
| 5 |
NC_000085.6 |
19 |
10423298 |
- |
Syt7 |
NNNGGGTGGCCAGTCAAAGA |
NGA |
2 |
0.0651 |
Tier II |
| 6 |
NC_000081.6 |
15 |
89531477 |
+ |
Shank3 |
NNNGGGTGTCCAGTGAAAGG |
NGG |
2 |
0.0267 |
Tier II |
| 7 |
NC_000070.6 |
4 |
128151570 |
+ |
Csmd2 |
NNNGTGTGGCCAGTGAAAGG |
NGG |
2 |
0.015 |
Tier II |
| 8 |
NC_000080.6 |
14 |
29432855 |
+ |
Cacna2d3 |
NNNGGGTGGACAGGCAAAGG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000080.6 |
14 |
51438022 |
+ |
Gm7247 |
NNNGGGTGGCCAGGCAATGG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000084.6 |
18 |
80615285 |
- |
Nfatc1 |
NNNGGGTGGCCAGGTAAAGG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000071.6 |
5 |
111427553 |
+ |
Mn1 |
NNNGGGTTGCCAGTCCAAGG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000076.6 |
10 |
122543024 |
- |
Gm40791 |
NNNGGGTGGCCAGACAAAGG |
NGG |
1 |
0.619 |
Tier III |
| 13 |
NC_000073.6 |
7 |
61236342 |
+ |
A230006K03Rik |
NNNAGGTTGCCAGTCAAAGG |
NGG |
2 |
0.5625 |
Tier III |
| 14 |
NC_000078.6 |
12 |
8069729 |
- |
Gm33037 |
NNNTGTTGGCCAGTCAAAGG |
NGG |
2 |
0.2424 |
Tier III |
| 15 |
NC_000070.6 |
4 |
128151570 |
+ |
Csmd2os |
NNNGTGTGGCCAGTGAAAGG |
NGG |
2 |
0.015 |
Tier III |
| 16 |
NC_000076.6 |
10 |
78092353 |
+ |
Gm30039 |
NNNGGGTGGCCAGGCAAATG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
