Construct: sgRNA BRDN0001149280
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTCCGATCATAATCAAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DLG3 (1741)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76158
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
70492115 |
- |
DLG3 |
NNNCCGATCATAATCAAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
118368277 |
+ |
SAMD12 |
NNNCAGATCATAATCAAATA |
NGG |
2 |
0.2637 |
Tier II |
| 3 |
NC_000017.11 |
17 |
57360121 |
- |
MSI2 |
NNNCCCATCATAATAAAACA |
NGG |
2 |
0.1515 |
Tier II |
| 4 |
NC_000002.12 |
2 |
28113947 |
- |
BABAM2 |
NNNCCAATCATAAGCAAACA |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000008.11 |
8 |
118368277 |
+ |
LOC105375724 |
NNNCAGATCATAATCAAATA |
NGG |
2 |
0.2637 |
Tier III |
| 6 |
NC_000005.10 |
5 |
103870157 |
+ |
LOC105379107 |
NNNCCGATAATAATAAAACA |
NGG |
2 |
0.1905 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
100806520 |
- |
Dlg3 |
NNNCCGATCATAATCAAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000075.6 |
9 |
4506805 |
+ |
Gria4 |
NNNCAGATCATAATCACACA |
NGG |
2 |
0.1008 |
Tier II |
| 3 |
NC_000083.6 |
17 |
21958388 |
+ |
Zfp942 |
NNNCCGATCATAATCAAGCA |
NGA |
2 |
0.0278 |
Tier II |
Other clones with same target sequence:
(none)
