Construct: sgRNA BRDN0001149298
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGACAAGCAGCCAATCGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRK5 (2869)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76272
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
119380853 |
+ |
GRK5 |
NNNACAAGCAGCCAATCGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
34976589 |
+ |
PHF24 |
NNNCCAAGCAGCCAAGCGGG |
NGG |
2 |
0.1222 |
Tier I |
| 3 |
NC_000008.11 |
8 |
2094244 |
- |
MYOM2 |
NNNACAAGCAGCCAACCGAG |
NGG |
2 |
0.4762 |
Tier II |
| 4 |
NC_000022.11 |
22 |
19987245 |
+ |
ARVCF |
NNNGCGAGCAGCCAATCGGG |
NGG |
2 |
0.4464 |
Tier II |
| 5 |
NC_000022.11 |
22 |
30930315 |
- |
MORC2 |
NNNACAAGCAGCTAATAGGG |
NGG |
2 |
0.3267 |
Tier II |
| 6 |
NC_000011.10 |
11 |
16940491 |
- |
PLEKHA7 |
NNNACCAGCAGCCAATCTGG |
NGG |
2 |
0.303 |
Tier II |
| 7 |
NC_000009.12 |
9 |
127215608 |
- |
RALGPS1 |
NNNACAAGCAGACAATCGGG |
NGA |
2 |
0.0496 |
Tier II |
| 8 |
NC_000004.12 |
4 |
168284484 |
+ |
DDX60 |
NNNACATGCAGCCAATCGGG |
NGT |
2 |
0.0071 |
Tier II |
| 9 |
NC_000014.9 |
14 |
105620848 |
- |
IGH |
NNNACAAGCAGCCACTCTGG |
NGG |
2 |
0.1818 |
Tier III |
| 10 |
NC_000014.9 |
14 |
105639900 |
- |
IGH |
NNNACAAGCAGCCACTCTGG |
NGG |
2 |
0.1818 |
Tier III |
| 11 |
NC_000014.9 |
14 |
105663560 |
- |
IGH |
NNNACAAGCAGCCACTCTGG |
NGG |
2 |
0.1818 |
Tier III |
| 12 |
NC_000014.9 |
14 |
105736688 |
- |
IGH |
NNNACAAGCAGCCACTCTGG |
NGG |
2 |
0.1818 |
Tier III |
| 13 |
NC_000014.9 |
14 |
105764848 |
- |
IGH |
NNNACAAGCAGCCACTCTGG |
NGG |
2 |
0.1818 |
Tier III |
| 14 |
NC_000014.9 |
14 |
105764848 |
- |
LOC105378184 |
NNNACAAGCAGCCACTCTGG |
NGG |
2 |
0.1818 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
141630529 |
- |
Ap2a2 |
NNNACAAGCAGCCAATCTGG |
NTG |
2 |
0.026 |
Tier I |
| 2 |
NC_000068.7 |
2 |
53069880 |
- |
Fmnl2 |
NNNACTAGCAGCCAATCTGG |
NGG |
2 |
0.4762 |
Tier II |
| 3 |
NC_000082.6 |
16 |
18392556 |
- |
Arvcf |
NNNACCAGCAGCCAATCGGG |
NGG |
1 |
0.4545 |
Tier II |
| 4 |
NC_000077.6 |
11 |
82813838 |
+ |
Rffl |
NNNACAAGCAGCCAATGGGA |
NGG |
2 |
0.0551 |
Tier II |
| 5 |
NC_000077.6 |
11 |
76370600 |
+ |
Nxn |
NNNACAAGCAGCCAATAGGG |
NGC |
2 |
0.0104 |
Tier II |
| 6 |
NC_000075.6 |
9 |
68491128 |
+ |
4930502A04Rik |
NNNACAAGAAGCCAATGGGG |
NGG |
2 |
0.0504 |
Tier III |
Other clones with same target sequence:
(none)
