Construct: sgRNA BRDN0001149301
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGTACCCACCTTATCCCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PGM2L1 (283209)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77145
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
74398058 |
+ |
PGM2L1 |
NNNTACCCACCTTATCCCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
81535429 |
+ |
PPFIA2 |
NNNTACCCACCATATCTCAG |
NGG |
2 |
0.3733 |
Tier I |
| 3 |
NC_000015.10 |
15 |
57024834 |
- |
TCF12 |
NNNTACCTACCTTATCCCAA |
NGG |
2 |
0.8203 |
Tier II |
| 4 |
NC_000014.9 |
14 |
32554711 |
- |
AKAP6 |
NNNTACCCACCTTATACAAG |
NGG |
2 |
0.5385 |
Tier II |
| 5 |
NC_000005.10 |
5 |
177390905 |
- |
SLC34A1 |
NNNTACCCACCTTTTCCCAG |
NGG |
1 |
0.5333 |
Tier II |
| 6 |
NC_000004.12 |
4 |
93531490 |
+ |
GRID2 |
NNNTACTCACCTTATCCTAG |
NGG |
2 |
0.5223 |
Tier II |
| 7 |
NC_000020.11 |
20 |
19483247 |
+ |
SLC24A3 |
NNNTACCCACCCTATCCCTG |
NGG |
2 |
0.5101 |
Tier II |
| 8 |
NC_000001.11 |
1 |
169699491 |
+ |
SELL |
NNNTAGCCACCTTATCCCAG |
NGG |
1 |
0.5 |
Tier II |
| 9 |
NC_000017.11 |
17 |
58576547 |
+ |
TEX14 |
NNNTACCCACTTTATCCCAG |
NGG |
1 |
0.3077 |
Tier II |
| 10 |
NC_000019.10 |
19 |
49873420 |
+ |
AKT1S1 |
NNNCACCCACCTTGTCCCAG |
NGG |
2 |
0.302 |
Tier II |
| 11 |
NC_000010.11 |
10 |
6169562 |
- |
PFKFB3 |
NNNTACCCATCTTATCCCAG |
NAG |
2 |
0.244 |
Tier II |
| 12 |
NC_000002.12 |
2 |
158463776 |
- |
PKP4 |
NNNTTCCCTCCTTATCCCAG |
NGG |
2 |
0.2182 |
Tier II |
| 13 |
NC_000001.11 |
1 |
33579492 |
+ |
CSMD2 |
NNNGACCCACCTTCTCCCAG |
NGG |
2 |
0.1339 |
Tier II |
| 14 |
NC_000007.14 |
7 |
155731298 |
+ |
RBM33 |
NNNTGCCCACCTTATCCGAG |
NGG |
2 |
0.096 |
Tier II |
| 15 |
NC_000002.12 |
2 |
47068682 |
+ |
TTC7A |
NNNTACCCACCTTATCCCTG |
NGC |
2 |
0.012 |
Tier II |
| 16 |
NC_000008.11 |
8 |
63869808 |
+ |
LINC01414 |
NNNTAGCCACATTATCCCAG |
NGG |
2 |
0.375 |
Tier III |
| 17 |
NC_000017.11 |
17 |
58576547 |
+ |
LOC107985048 |
NNNTACCCACTTTATCCCAG |
NGG |
1 |
0.3077 |
Tier III |
| 18 |
NC_000006.12 |
6 |
126970680 |
- |
LOC105377989 |
NNNTACCCACCTTAGGCCAG |
NGG |
2 |
0.0077 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
68950068 |
- |
Erbb4 |
NNNTACTCAACTTATCCCAG |
NGG |
2 |
0.7042 |
Tier II |
| 2 |
NC_000071.6 |
5 |
72805870 |
- |
Tec |
NNNTAGCCATCTTATCCCAG |
NGG |
2 |
0.4706 |
Tier II |
| 3 |
NC_000075.6 |
9 |
91371527 |
- |
Zic4 |
NNNTACTCACCTTTTCCCAG |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000068.7 |
2 |
127477721 |
+ |
Kcnip3 |
NNNTCCCCACCATATCCCAG |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000068.7 |
2 |
54636749 |
+ |
Galnt13 |
NNNTACCCACATTGTCCCAG |
NGG |
2 |
0.35 |
Tier II |
| 6 |
NC_000075.6 |
9 |
82898214 |
- |
Phip |
NNNTACCCACTTCATCCCAG |
NGG |
2 |
0.2429 |
Tier II |
| 7 |
NC_000081.6 |
15 |
97331271 |
+ |
Pced1b |
NNNTACCCAGCTTGTCCCAG |
NGG |
2 |
0.1815 |
Tier II |
| 8 |
NC_000068.7 |
2 |
163391769 |
+ |
Jph2 |
NNNTACCCACTTTATCCCAC |
NGG |
2 |
0.1319 |
Tier II |
| 9 |
NC_000083.6 |
17 |
46756836 |
+ |
Ptcra |
NNNTCCCCACCTTATCCCAG |
NGA |
2 |
0.0347 |
Tier II |
| 10 |
NC_000072.6 |
6 |
114457847 |
- |
Hrh1 |
NNNTCCCCACCTTATCCCAG |
NGT |
2 |
0.0081 |
Tier II |
| 11 |
NC_000078.6 |
12 |
7561455 |
- |
Gm32828 |
NNNTACCCAACCTATCCCAG |
NGG |
2 |
0.8211 |
Tier III |
| 12 |
NC_000067.6 |
1 |
68950068 |
- |
Gm51744 |
NNNTACTCAACTTATCCCAG |
NGG |
2 |
0.7042 |
Tier III |
| 13 |
NC_000078.6 |
12 |
4815466 |
- |
Gm20348 |
NNNTCCCCACCTTATCACAG |
NGG |
2 |
0.2333 |
Tier III |
| 14 |
NC_000067.6 |
1 |
72950705 |
+ |
Gm31047 |
NNNTACCCTCCTTATCCCAG |
NTG |
2 |
0.0234 |
Tier III |
| 15 |
NC_000067.6 |
1 |
55951419 |
- |
9130227L01Rik |
NNNTACCCACCTTAGTCCAG |
NGG |
2 |
0.0154 |
Tier III |
| 16 |
NC_000071.6 |
5 |
107402665 |
+ |
Gm17202 |
NNNTACCCACCTTATCCCAC |
NGT |
2 |
0.0069 |
Tier III |
Other clones with same target sequence:
(none)
