Construct: sgRNA BRDN0001149309
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATACTTTGAAGATCGTCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ITK (3702)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77446
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
157181111 |
+ |
ITK |
NNNCTTTGAAGATCGTCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
133133341 |
- |
NCKAP5 |
NNNCTTTGAAAATCATCATG |
NGG |
2 |
0.9412 |
Tier II |
| 3 |
NC_000001.11 |
1 |
7286373 |
+ |
CAMTA1 |
NNNCCTGGAAGATCGTCATG |
NGG |
2 |
0.5882 |
Tier II |
| 4 |
NC_000005.10 |
5 |
107942165 |
- |
FBXL17 |
NNNCTCTGAAGATGGTCATG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000004.12 |
4 |
66017825 |
+ |
LOC105377261 |
NNNCTATTAAGATCGTCATG |
NGG |
2 |
0.5417 |
Tier III |
| 6 |
NC_000015.10 |
15 |
97828812 |
- |
LINC00923 |
NNNCTGTGAAGATTGTCATG |
NGG |
2 |
0.419 |
Tier III |
| 7 |
NC_000001.11 |
1 |
189496297 |
- |
LOC105371657 |
NNNCTTTGAAGACCTTCATG |
NGG |
2 |
0.1128 |
Tier III |
| 8 |
NC_000002.12 |
2 |
12711875 |
- |
LOC124905974 |
NNNCTTTGAAGATAGTCATG |
NTG |
2 |
0.0136 |
Tier III |
| 9 |
NC_000005.10 |
5 |
58108820 |
- |
LINC02101 |
NNNCTTTGAAGATGGTCATG |
NGG |
1 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
106366827 |
- |
Phlpp1 |
NNNCTTTGAAGATAGTCATT |
NGG |
2 |
0.245 |
Tier II |
| 2 |
NC_000067.6 |
1 |
81220261 |
- |
Nyap2 |
NNNCTTTGAAGAAAGTCATG |
NGG |
2 |
0.2423 |
Tier II |
| 3 |
NC_000069.6 |
3 |
145152135 |
- |
Odf2l |
NNNCTGTGAAGATAGTCATG |
NGG |
2 |
0.2 |
Tier II |
| 4 |
NC_000074.6 |
8 |
81522017 |
- |
Inpp4b |
NNNCTTTGAAGATAGTAATG |
NGG |
2 |
0.1633 |
Tier II |
| 5 |
NC_000070.6 |
4 |
96498901 |
- |
Cyp2j8 |
NNNCTTTGTAGATCTTCATG |
NGG |
2 |
0.0857 |
Tier II |
| 6 |
NC_000071.6 |
5 |
54052678 |
+ |
Stim2 |
NNNCTTTGCAGATCTTCATG |
NGG |
2 |
0.0816 |
Tier II |
| 7 |
NC_000067.6 |
1 |
69584814 |
- |
Ikzf2 |
NNNCTTTGAAGATCGTAATG |
NTG |
2 |
0.0182 |
Tier II |
| 8 |
NC_000075.6 |
9 |
71188977 |
- |
Gm32511 |
NNNCTTAGAAGATCCTCATG |
NGG |
2 |
0.2386 |
Tier III |
Other clones with same target sequence:
(none)
