Construct: sgRNA BRDN0001149310
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACCTACAGGAGTACCTGACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TGFBR2 (7048)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77469
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
30672195 |
+ |
TGFBR2 |
NNNTACAGGAGTACCTGACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
139573414 |
- |
UBE2D2 |
NNNTAAAGGAGTACCTGATG |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000002.12 |
2 |
1055090 |
- |
SNTG2 |
NNNCACAGCAGTACCTGACG |
NGG |
2 |
0.3484 |
Tier II |
| 4 |
NC_000014.9 |
14 |
95233710 |
- |
CLMN |
NNNTGCAGGAGTACCTCACG |
NGG |
2 |
0.1694 |
Tier II |
| 5 |
NC_000020.11 |
20 |
26193412 |
+ |
MIR663AHG |
NNNTACAGGAACACCTGACG |
NGG |
2 |
0.9474 |
Tier III |
| 6 |
NC_000007.14 |
7 |
130880733 |
- |
LINC-PINT |
NNNCACAGGAGTACCTGGCG |
NGG |
2 |
0.2588 |
Tier III |
| 7 |
NC_000011.10 |
11 |
115677318 |
+ |
LINC02698 |
NNNTACAGGAGATCCTGACG |
NGG |
2 |
0.24 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
116109747 |
- |
Tgfbr2 |
NNNTGCAGGAGTACCTCACG |
NGG |
2 |
0.1694 |
Tier I |
| 2 |
NC_000075.6 |
9 |
24640183 |
+ |
Dpy19l2 |
NNNTAGAGGAGTATCTGACG |
NGG |
2 |
0.3667 |
Tier II |
| 3 |
NC_000074.6 |
8 |
23419963 |
+ |
Sfrp1 |
NNNTTCAGGAGAACCTGACG |
NGG |
2 |
0.2909 |
Tier II |
| 4 |
NC_000079.6 |
13 |
17294374 |
+ |
Sugct |
NNNTACAGGAGTTCCTGACT |
NGG |
2 |
0.21 |
Tier II |
| 5 |
NC_000072.6 |
6 |
30288362 |
+ |
Ube2h |
NNNTACAGGAGTACATGAAG |
NGG |
2 |
0.0952 |
Tier II |
| 6 |
NC_000079.6 |
13 |
23299272 |
- |
4930557F10Rik |
NNNTAAACGAGTACCTGACG |
NGG |
2 |
0.5714 |
Tier III |
Other clones with same target sequence:
(none)
