Construct: sgRNA BRDN0001149313

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCATCAAATCCAATTATCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRPK1 (6732)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77494

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 35874275 - SRPK1 NNNTCAAATCCAATTATCAG NGG 0 1.0 Tier I
2 NC_000010.11 10 120489150 + PLPP4 NNNTCAAATCCAATTCTCAG NGA 2 0.0 Tier I
3 NC_000009.12 9 28884310 - LINGO2 NNNTCAAATACAATTATCAA NGG 2 0.8125 Tier II
4 NC_000007.14 7 147225232 + CNTNAP2 NNNACAAATTCAATTATCAG NGG 2 0.4482 Tier II
5 NC_000009.12 9 124412533 + PSMB7 NNNTGAAATCCAATTACCAG NGG 2 0.4235 Tier II
6 NC_000012.12 12 1049452 - ERC1 NNNTCAAATCAAATTATCTG NGG 2 0.4038 Tier II
7 NC_000001.11 1 50086774 - ELAVL4 NNNTCCAATCCAATTATCTG NGG 2 0.2448 Tier II
8 NC_000003.12 3 41302247 - ULK4 NNNTCAAAGGCAATTATCAG NGG 2 0.2407 Tier II
9 NC_000012.12 12 55027959 + NEUROD4 NNNTCAAATTGAATTATCAG NGG 2 0.2353 Tier II
10 NC_000005.10 5 139067410 - SIL1 NNNTCAAATCCTGTTATCAG NGG 2 0.2174 Tier II
11 NC_000023.11 X 130136311 - AIFM1 NNNTAAAATCCATTTATCAG NGG 2 0.1714 Tier II
12 NC_000023.11 X 130136311 - RAB33A NNNTAAAATCCATTTATCAG NGG 2 0.1714 Tier II
13 NC_000009.12 9 74573579 + RORB NNNTCAAATTCAATTAGCAG NGG 2 0.1107 Tier II
14 NC_000023.11 X 29636282 + IL1RAPL1 NNNTAAAATCCAATTATGAG NGG 2 0.0762 Tier II
15 NC_000001.11 1 210386150 + HHAT NNNTCAAATCCAATTAAGAG NGG 2 0.0711 Tier II
16 NC_000002.12 2 62697426 - EHBP1 NNNTAAAATCCAATTATCAG NTG 2 0.0223 Tier II
17 NC_000013.11 13 113808050 - TMEM255B NNNTGAAATCCAATTATCAG NGT 2 0.0097 Tier II
18 NC_000005.10 5 14468735 - TRIO NNNTCAAATCCAATTATGAG NGT 2 0.0022 Tier II
19 NC_000023.11 X 68413370 + OPHN1 NNNTCAAAGCCAATTCTCAG NGG 2 0.0 Tier II
20 NC_000007.14 7 148275133 + CNTNAP2 NNNTCAAATCCAGTTCTCAG NGG 2 0.0 Tier II
21 NC_000004.12 4 158961540 - SPMIP2 NNNTCAAATTCAATTTTCAG NGG 2 0.0 Tier II
22 NC_000011.10 11 39752491 + LOC105376637 NNNTCAAATTCAAATATCAG NGG 2 0.5826 Tier III
23 NC_000013.11 13 78046073 - LINC00446 NNNTAAAATCCAATTATCAA NGG 2 0.5357 Tier III
24 NC_000012.12 12 55027959 + LOC101927484 NNNTCAAATTGAATTATCAG NGG 2 0.2353 Tier III
25 NC_000003.12 3 131406020 + LOC107986025 NNNACAAATCTAATTATCAG NGG 2 0.1465 Tier III
26 NC_000003.12 3 6838098 + LOC105376945 NNNTCAAATCCAATGAACAG NGG 2 0.0267 Tier III
27 NC_000005.10 5 125678120 + LOC124901056 NNNTCAAATCCATTTATCAG NTG 2 0.0117 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 28603480 - Srpk1 NNNTCAAGTCCAACTATCAG NGG 2 0.2095 Tier I
2 NC_000075.6 9 59789303 + Myo9a NNNTCAAATCCAATTATTAG NGG 1 0.6429 Tier II
3 NC_000086.7 X 86785092 - Il1rapl1 NNNTAAAAGCCAATTATCAG NGG 2 0.3537 Tier II
4 NC_000072.6 6 39382028 + Rab19 NNNTCAAATCCAATCATCAA NGG 2 0.2557 Tier II
5 NC_000085.6 19 34118046 - Lipm NNNTCATATCCAATAATCAG NGG 2 0.2533 Tier II
6 NC_000083.6 17 3140810 - Scaf8 NNNTCAGATCCAACTATCAG NGG 2 0.2017 Tier II
7 NC_000079.6 13 76662442 + Mctp1 NNNTAAAATCCACTTATCAG NGG 2 0.1203 Tier II
8 NC_000068.7 2 166969480 + Stau1 NNNTCAAATACAATTATGAG NGG 2 0.1156 Tier II
9 NC_000077.6 11 66245728 + Shisa6 NNNCCAAATCCAATTAGCAG NGG 2 0.0761 Tier II
10 NC_000082.6 16 35711109 + Slc49a4 NNNTCAAATCCATTTGTCAG NGG 2 0.0577 Tier II
11 NC_000076.6 10 79152434 + Vmn2r80 NNNTAAAATCCAATTATCAG NGA 2 0.0397 Tier II
12 NC_000070.6 4 98603503 + Patj NNNTCGAATCCAATGATCAG NGG 2 0.0357 Tier II
13 NC_000083.6 17 72047312 + Alk NNNTCCAATCCAATTATCAG NTG 2 0.0177 Tier II
14 NC_000075.6 9 114638485 - Cnot10 NNNTCAAATCCAATCATCAG NGC 2 0.0061 Tier II
15 NC_000079.6 13 47180595 - Rnf144b NNNTCAAATCCAATTATGAG NTG 2 0.0052 Tier II
16 NC_000076.6 10 94525741 + Tmcc3 NNNTCAAATCCAAATCTCAG NGG 2 0.0 Tier II
17 NC_000073.6 7 44054125 + Klk1b21 NNNTCAAATCCAATTTTCTG NGG 2 0.0 Tier II
18 NC_000073.6 7 44054125 + Klk1b27 NNNTCAAATCCAATTTTCTG NGG 2 0.0 Tier II
19 NC_000073.6 7 44103981 + Klk1b21 NNNTCAAATCCAATTTTCTG NGG 2 0.0 Tier II
20 NC_000073.6 7 85530412 + Gm18360 NNNTTAAACCCAATTATCAG NGG 2 0.5874 Tier III
21 NC_000079.6 13 103625429 + Gm30551 NNNTAAAATCCATTTATCAG NGG 2 0.1714 Tier III
22 NC_000069.6 3 149587771 + Gm31121 NNNTAAAATCCAATTATCAG NAG 2 0.1481 Tier III
23 NC_000077.6 11 66245728 + Gm12296 NNNCCAAATCCAATTAGCAG NGG 2 0.0761 Tier III
24 NC_000074.6 8 115581431 - Gm39256 NNNTCAAATCCAATTTTTAG NGG 2 0.0 Tier III
25 NC_000076.6 10 19064566 - Gm51814 NNNTCAAATTCAATTCTCAG NGG 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.