Construct: sgRNA BRDN0001149313

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCATCAAATCCAATTATCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRPK1 (6732)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77494

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 35874275 - SRPK1 NNNTCAAATCCAATTATCAG NGG 0 1.0 Tier I
2 NC_000010.11 10 120489150 + PLPP4 NNNTCAAATCCAATTCTCAG NGA 2 0.0 Tier I
3 NC_000009.12 9 28884310 - LINGO2 NNNTCAAATACAATTATCAA NGG 2 0.8125 Tier II
4 NC_000007.14 7 147225232 + CNTNAP2 NNNACAAATTCAATTATCAG NGG 2 0.4482 Tier II
5 NC_000009.12 9 124412533 + PSMB7 NNNTGAAATCCAATTACCAG NGG 2 0.4235 Tier II
6 NC_000012.12 12 1049452 - ERC1 NNNTCAAATCAAATTATCTG NGG 2 0.4038 Tier II
7 NC_000001.11 1 50086774 - ELAVL4 NNNTCCAATCCAATTATCTG NGG 2 0.2448 Tier II
8 NC_000003.12 3 41302247 - ULK4 NNNTCAAAGGCAATTATCAG NGG 2 0.2407 Tier II
9 NC_000012.12 12 55027959 + NEUROD4 NNNTCAAATTGAATTATCAG NGG 2 0.2353 Tier II
10 NC_000005.10 5 139067410 - SIL1 NNNTCAAATCCTGTTATCAG NGG 2 0.2174 Tier II
11 NC_000023.11 X 130136311 - AIFM1 NNNTAAAATCCATTTATCAG NGG 2 0.1714 Tier II
12 NC_000023.11 X 130136311 - RAB33A NNNTAAAATCCATTTATCAG NGG 2 0.1714 Tier II
13 NC_000009.12 9 74573579 + RORB NNNTCAAATTCAATTAGCAG NGG 2 0.1107 Tier II
14 NC_000023.11 X 29636282 + IL1RAPL1 NNNTAAAATCCAATTATGAG NGG 2 0.0762 Tier II
15 NC_000001.11 1 210386150 + HHAT NNNTCAAATCCAATTAAGAG NGG 2 0.0711 Tier II
16 NC_000002.12 2 62697426 - EHBP1 NNNTAAAATCCAATTATCAG NTG 2 0.0223 Tier II
17 NC_000013.11 13 113808050 - TMEM255B NNNTGAAATCCAATTATCAG NGT 2 0.0097 Tier II
18 NC_000005.10 5 14468735 - TRIO NNNTCAAATCCAATTATGAG NGT 2 0.0022 Tier II
19 NC_000023.11 X 68413370 + OPHN1 NNNTCAAAGCCAATTCTCAG NGG 2 0.0 Tier II
20 NC_000007.14 7 148275133 + CNTNAP2 NNNTCAAATCCAGTTCTCAG NGG 2 0.0 Tier II
21 NC_000004.12 4 158961540 - SPMIP2 NNNTCAAATTCAATTTTCAG NGG 2 0.0 Tier II
22 NC_000011.10 11 39752491 + LOC105376637 NNNTCAAATTCAAATATCAG NGG 2 0.5826 Tier III
23 NC_000013.11 13 78046073 - LINC00446 NNNTAAAATCCAATTATCAA NGG 2 0.5357 Tier III
24 NC_000012.12 12 55027959 + LOC101927484 NNNTCAAATTGAATTATCAG NGG 2 0.2353 Tier III
25 NC_000003.12 3 131406020 + LOC107986025 NNNACAAATCTAATTATCAG NGG 2 0.1465 Tier III
26 NC_000003.12 3 6838098 + LOC105376945 NNNTCAAATCCAATGAACAG NGG 2 0.0267 Tier III
27 NC_000005.10 5 125678120 + LOC124901056 NNNTCAAATCCATTTATCAG NTG 2 0.0117 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 28603480 - Srpk1 NNNTCAAGTCCAACTATCAG NGG 2 0.2095 Tier I
2 NC_000075.6 9 59789303 + Myo9a NNNTCAAATCCAATTATTAG NGG 1 0.6429 Tier II
3 NC_000086.7 X 86785092 - Il1rapl1 NNNTAAAAGCCAATTATCAG NGG 2 0.3537 Tier II
4 NC_000072.6 6 39382028 + Rab19 NNNTCAAATCCAATCATCAA NGG 2 0.2557 Tier II
5 NC_000085.6 19 34118046 - Lipm NNNTCATATCCAATAATCAG NGG 2 0.2533 Tier II
6 NC_000083.6 17 3140810 - Scaf8 NNNTCAGATCCAACTATCAG NGG 2 0.2017 Tier II
7 NC_000079.6 13 76662442 + Mctp1 NNNTAAAATCCACTTATCAG NGG 2 0.1203 Tier II
8 NC_000068.7 2 166969480 + Stau1 NNNTCAAATACAATTATGAG NGG 2 0.1156 Tier II
9 NC_000077.6 11 66245728 + Shisa6 NNNCCAAATCCAATTAGCAG NGG 2 0.0761 Tier II
10 NC_000082.6 16 35711109 + Slc49a4 NNNTCAAATCCATTTGTCAG NGG 2 0.0577 Tier II
11 NC_000076.6 10 79152434 + Vmn2r80 NNNTAAAATCCAATTATCAG NGA 2 0.0397 Tier II
12 NC_000070.6 4 98603503 + Patj NNNTCGAATCCAATGATCAG NGG 2 0.0357 Tier II
13 NC_000083.6 17 72047312 + Alk NNNTCCAATCCAATTATCAG NTG 2 0.0177 Tier II
14 NC_000075.6 9 114638485 - Cnot10 NNNTCAAATCCAATCATCAG NGC 2 0.0061 Tier II
15 NC_000079.6 13 47180595 - Rnf144b NNNTCAAATCCAATTATGAG NTG 2 0.0052 Tier II
16 NC_000076.6 10 94525741 + Tmcc3 NNNTCAAATCCAAATCTCAG NGG 2 0.0 Tier II
17 NC_000073.6 7 44054125 + Klk1b21 NNNTCAAATCCAATTTTCTG NGG 2 0.0 Tier II
18 NC_000073.6 7 44054125 + Klk1b27 NNNTCAAATCCAATTTTCTG NGG 2 0.0 Tier II
19 NC_000073.6 7 44103981 + Klk1b21 NNNTCAAATCCAATTTTCTG NGG 2 0.0 Tier II
20 NC_000073.6 7 85530412 + Gm18360 NNNTTAAACCCAATTATCAG NGG 2 0.5874 Tier III
21 NC_000079.6 13 103625429 + Gm30551 NNNTAAAATCCATTTATCAG NGG 2 0.1714 Tier III
22 NC_000069.6 3 149587771 + Gm31121 NNNTAAAATCCAATTATCAG NAG 2 0.1481 Tier III
23 NC_000077.6 11 66245728 + Gm12296 NNNCCAAATCCAATTAGCAG NGG 2 0.0761 Tier III
24 NC_000074.6 8 115581431 - Gm39256 NNNTCAAATCCAATTTTTAG NGG 2 0.0 Tier III
25 NC_000076.6 10 19064566 - Gm51814 NNNTCAAATTCAATTCTCAG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)