Construct: sgRNA BRDN0001149314
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGGACTTTCGGGAACGAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PFKM (5213)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77077
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
48132915 |
+ |
PFKM |
NNNGACTTTCGGGAACGAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000024.10 |
Y |
5097653 |
+ |
PCDH11Y |
NNNGACTTTGGGGAAAGAGA |
NGG |
2 |
0.3889 |
Tier II |
| 3 |
NC_000010.11 |
10 |
106626340 |
+ |
SORCS1 |
NNNGACTTTTGGGAACGAGA |
NGT |
2 |
0.0152 |
Tier II |
| 4 |
NC_000008.11 |
8 |
126914501 |
- |
LOC105375751 |
NNNCACTTTCGGGAACCAGA |
NGG |
2 |
0.1246 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
125244402 |
+ |
Disc1 |
NNNGACTTTAGGGAAAGAGA |
NGG |
2 |
0.8667 |
Tier II |
| 2 |
NC_000079.6 |
13 |
113173567 |
- |
Gzmk |
NNNGACTTCAGGGAACGAGA |
NGG |
2 |
0.8 |
Tier II |
| 3 |
NC_000084.6 |
18 |
10589564 |
- |
Esco1 |
NNNGACTTTCGGGAGAGAGA |
NGG |
2 |
0.65 |
Tier II |
| 4 |
NC_000073.6 |
7 |
116514030 |
- |
Nucb2 |
NNNCACTTTCGGGAACGAAA |
NGG |
2 |
0.3782 |
Tier II |
| 5 |
NC_000077.6 |
11 |
64472998 |
+ |
Hs3st3a1 |
NNNGACTTTCTGGAATGAGA |
NGG |
2 |
0.1183 |
Tier II |
Other clones with same target sequence:
(none)
