Construct: sgRNA BRDN0001149315
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCGGGTTCTTACCGTATGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK1A1 (1452)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76188
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
149550080 |
+ |
CSNK1A1 |
NNNGGTTCTTACCGTATGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
61741988 |
+ |
CABCOCO1 |
NNNGGTTCTTACCAAATGTG |
NGG |
2 |
0.4342 |
Tier II |
| 3 |
NC_000002.12 |
2 |
133194390 |
- |
NCKAP5 |
NNNTGTTCCTACCGTATGTG |
NGG |
2 |
0.3357 |
Tier II |
| 4 |
NC_000012.12 |
12 |
47223272 |
- |
PCED1B |
NNNGGTTCTTACAGTATGAG |
NGG |
2 |
0.1099 |
Tier II |
| 5 |
NC_000001.11 |
1 |
32601515 |
+ |
ZBTB8A |
NNNGGTTCTTACCATATGTG |
NGT |
2 |
0.0121 |
Tier II |
| 6 |
NC_000003.12 |
3 |
138900971 |
+ |
LOC124906289 |
NNNGGTGCTTACCATATGTG |
NGG |
2 |
0.4412 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
61556605 |
- |
Csnk1a1 |
NNNGGTGCTTACCGTATGTG |
NGG |
1 |
0.5882 |
Tier I |
| 2 |
NC_000080.6 |
14 |
77664462 |
+ |
Enox1 |
NNNGCTCCTTACCGTATGTG |
NGG |
2 |
0.5402 |
Tier II |
| 3 |
NC_000075.6 |
9 |
107516680 |
- |
Cacna2d2 |
NNNGTTTCTTACTGTATGTG |
NGG |
2 |
0.21 |
Tier II |
| 4 |
NC_000068.7 |
2 |
168570101 |
+ |
Nfatc2 |
NNNGGTTCTTACACTATGTG |
NGG |
2 |
0.1648 |
Tier II |
| 5 |
NC_000076.6 |
10 |
79880120 |
+ |
Prtn3 |
NNNGGTTCTTACTGTATGTG |
NGC |
2 |
0.0156 |
Tier II |
| 6 |
NC_000080.6 |
14 |
122250110 |
+ |
Clybl |
NNNGGTTTTTACCGTTTGTG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000077.6 |
11 |
83255408 |
- |
Gm11427 |
NNNGGGTCTTACAGTATGTG |
NGG |
2 |
0.2198 |
Tier III |
Other clones with same target sequence:
(none)
