Construct: sgRNA BRDN0001149316

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGAAATCAGCCAGTGCAGGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EXOSC10 (5394)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77990

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 11090608 + EXOSC10 NNNAATCAGCCAGTGCAGGG NGG 0 1.0 Tier I
2 NC_000002.12 2 230934164 - GPR55 NNNAAACAGCCAGTGCAGGG NGG 1 0.8667 Tier II
3 NC_000021.9 21 29835620 + GRIK1 NNNAAACAGTCAGTGCAGGG NGG 2 0.8157 Tier II
4 NC_000001.11 1 31743869 + ADGRB2 NNNAATCAGCAAATGCAGGG NGG 2 0.6923 Tier II
5 NC_000009.12 9 138120494 + CACNA1B NNNAACCAGCCAGAGCAGGG NGG 2 0.5628 Tier II
6 NC_000013.11 13 42589737 + TNFSF11 NNNAATCAGCAAGTGCAGAG NGG 2 0.5357 Tier II
7 NC_000004.12 4 76315054 - CCDC158 NNNAGGCAGCCAGTGCAGGG NGG 2 0.4114 Tier II
8 NC_000007.14 7 142862494 - EPHB6 NNNAATGAGACAGTGCAGGG NGG 2 0.4078 Tier II
9 NC_000017.11 17 17554482 - PEMT NNNAAGCAGCCAGAGCAGGG NGG 2 0.3537 Tier II
10 NC_000002.12 2 62499521 + TMEM17 NNNAATCAGCCACTGCATGG NGG 2 0.2807 Tier II
11 NC_000022.11 22 19776946 + TBX1 NNNGATCAGCCAGTGCAGGC NGG 2 0.2679 Tier II
12 NC_000019.10 19 51529555 + SIGLEC6 NNNAAGCTGCCAGTGCAGGG NGG 2 0.2449 Tier II
13 NC_000014.9 14 64152289 + SYNE2 NNNAAACAGCCAGTGCAGGG NAG 2 0.2247 Tier II
14 NC_000011.10 11 17993104 + SERGEF NNNACTCAGCCAGTGCAGGC NGG 2 0.2143 Tier II
15 NC_000019.10 19 44352505 + ZNF112 NNNAATCAGCCAGTGTAGTG NGG 2 0.2051 Tier II
16 NC_000007.14 7 22121431 - RAPGEF5 NNNCAGCAGCCAGTGCAGGG NGG 2 0.2017 Tier II
17 NC_000011.10 11 8388720 + STK33 NNNAAGCAGCCAGTGTAGGG NGG 2 0.1758 Tier II
18 NC_000018.10 18 32699904 + KLHL14 NNNAATCAGCCAGTTAAGGG NGG 2 0.1429 Tier II
19 NC_000004.12 4 25002197 - LGI2 NNNAATCAGCCTGTGCAGGC NGG 2 0.1429 Tier II
20 NC_000015.10 15 38530158 + RASGRP1 NNNAATCAGCTAGTGCAGGC NGG 2 0.1319 Tier II
21 NC_000003.12 3 141147683 + SPSB4 NNNAATTAGCCAGTGCAGGG NGA 2 0.0564 Tier II
22 NC_000001.11 1 3327830 - PRDM16 NNNCATCAGCCAGTGCTGGG NGG 2 0.0471 Tier II
23 NC_000003.12 3 52792066 - ITIH1 NNNAATCAGCCAGCTCAGGG NGG 2 0.0408 Tier II
24 NC_000011.10 11 5486738 - OR51B5 NNNCATCAGCCAGTGCAGGG NTG 2 0.0138 Tier II
25 NC_000008.11 8 30722590 + GSR NNNAATCAGCCAGGGCATGG NGG 2 0.0 Tier II
26 NC_000004.12 4 102914248 + SLC9B1 NNNAATCAGCCAGTGCAGGG NCT 2 0.0 Tier II
27 NC_000015.10 15 41992706 + PLA2G4E NNNCATCAGCCAGGGCAGGG NGG 2 0.0 Tier II
28 NC_000009.12 9 118868455 + LOC101928849 NNNAATGAGCCAGTGAAGGG NGG 2 0.4706 Tier III
29 NC_000004.12 4 136918355 + LINC02511 NNNAATCAGCCAGAGCAGAG NGG 2 0.4422 Tier III
30 NC_000002.12 2 158724103 + PKP4-AS1 NNNAAACAGCCAGTGCAGGC NGG 2 0.3714 Tier III
31 NC_000004.12 4 137560618 + LINC02172 NNNAAACAGCCACTGCAGGG NGG 2 0.3649 Tier III
32 NC_000003.12 3 6693052 + GRM7-AS3 NNNCATCAGACAGTGCAGGG NGG 2 0.3059 Tier III
33 NC_000003.12 3 6693052 + LOC105376944 NNNCATCAGACAGTGCAGGG NGG 2 0.3059 Tier III
34 NC_000003.12 3 6693052 + YWHAQP10 NNNCATCAGACAGTGCAGGG NGG 2 0.3059 Tier III
35 NC_000007.14 7 106819499 - LINC02577 NNNAATAAGCCAGTCCAGGG NGG 2 0.2045 Tier III
36 NC_000018.10 18 32699904 + LOC112268208 NNNAATCAGCCAGTTAAGGG NGG 2 0.1429 Tier III
37 NC_000004.12 4 25002197 - LOC102723675 NNNAATCAGCCTGTGCAGGC NGG 2 0.1429 Tier III
38 NC_000013.11 13 22065055 + LOC105370108 NNNAATCTGCCTGTGCAGGG NGG 2 0.1429 Tier III
39 NC_000017.11 17 52886106 - LINC02089 NNNAATCAGCAAGTGCAGGG NGC 2 0.0167 Tier III
40 NC_000011.10 11 112499701 + LINC02763 NNNAATCAGCCAGTGCAGGG NTC 2 0.0 Tier III
41 NC_000015.10 15 41992706 + PLA2G4E-AS1 NNNCATCAGCCAGGGCAGGG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 54276116 - Trpc4 NNNAACCAGCCAGTACAGGG NGG 2 0.8556 Tier II
2 NC_000075.6 9 7046317 - Dync2h1 NNNAACCAGCCAGTACAGGG NGG 2 0.8556 Tier II
3 NC_000077.6 11 107756519 + Cacng4 NNNAACCAGCCAGTACAGGG NGG 2 0.8556 Tier II
4 NC_000082.6 16 31675643 + Dlg1 NNNAACCAGCCAGTACAGGG NGG 2 0.8556 Tier II
5 NC_000083.6 17 33384906 - Zfp101 NNNAACCAGCCAGTACAGGG NGG 2 0.8556 Tier II
6 NC_000084.6 18 64610056 - Atp8b1 NNNAACCAGCCAGTACAGGG NGG 2 0.8556 Tier II
7 NC_000077.6 11 32015084 + Nsg2 NNNAACCAGCCAGTGCAGGA NGG 2 0.8523 Tier II
8 NC_000070.6 4 155430923 - Cfap74 NNNAATCAGTCAGTGCAGAG NGG 2 0.6723 Tier II
9 NC_000072.6 6 27744510 - Grm8 NNNAATCAGCCAGTACAGGT NGG 2 0.6588 Tier II
10 NC_000079.6 13 95132557 - Pde8b NNNAATCAGCCAGTACAGGT NGG 2 0.6588 Tier II
11 NC_000079.6 13 76531345 + Mctp1 NNNAAGCAGCCAGTGAAGGG NGG 2 0.5714 Tier II
12 NC_000076.6 10 86885890 - Stab2 NNNAATTAGCCAGTGCAGTG NGG 2 0.5417 Tier II
13 NC_000086.7 X 160037363 - Map3k15 NNNAAGCAGCCGGTGCAGGG NGG 2 0.4127 Tier II
14 NC_000077.6 11 97681773 - Mllt6 NNNAAGCAGCCAGTGCAGTG NGG 2 0.381 Tier II
15 NC_000086.7 X 98204675 + Ar NNNAATTAGGCAGTGCAGGG NGG 2 0.316 Tier II
16 NC_000068.7 2 119052719 - Knl1 NNNATTCAGACAGTGCAGGG NGG 2 0.3152 Tier II
17 NC_000081.6 15 4071532 - Oxct1 NNNAACCAGCCAGTGCAGGG NAG 2 0.2357 Tier II
18 NC_000068.7 2 161062415 + Chd6 NNNAAGCAGGCAGTGCAGGG NGG 2 0.2222 Tier II
19 NC_000073.6 7 19084349 + Dmpk NNNACTCAGCCAGTGCAGGC NGG 2 0.2143 Tier II
20 NC_000080.6 14 103662126 + Slain1 NNNAATGAGCCACTGCAGGG NGG 2 0.1981 Tier II
21 NC_000069.6 3 58618083 + Erich6 NNNGATCAGCCAGTGTAGGG NGG 2 0.1923 Tier II
22 NC_000077.6 11 49244356 + Mgat1 NNNAAGCAGCCCGTGCAGGG NGG 2 0.1504 Tier II
23 NC_000075.6 9 29523472 - Ntm NNNAATCACCCCGTGCAGGG NGG 2 0.1417 Tier II
24 NC_000068.7 2 33613585 - Lmx1b NNNAATCAGGCAGTCCAGGG NGG 2 0.1061 Tier II
25 NC_000070.6 4 14746432 + Lrrc69 NNNAATCAGCCAGTGCAGGT NGA 2 0.0486 Tier II
26 NC_000071.6 5 112533015 - Sez6l NNNAAGCAGCCAGTGCAGGG NGA 2 0.0397 Tier II
27 NC_000068.7 2 125325360 - Fbn1 NNNCATCAGCCAGTGCAGGG NGA 2 0.0245 Tier II
28 NC_000082.6 16 90421407 - Hunk NNNAATCAGCCACTGCAGGG NTG 2 0.0164 Tier II
29 NC_000080.6 14 29198642 - Cacna2d3 NNNAATCAGCCAGTGAAGGG NGT 2 0.0161 Tier II
30 NC_000076.6 10 41262143 - Fig4 NNNAATCAGCCAGTACAGGG NGT 2 0.0152 Tier II
31 NC_000082.6 16 11468798 - Snx29 NNNAATCAGCCAGGGCAGGG NTG 2 0.0 Tier II
32 NC_000081.6 15 99498931 + Faim2 NNNAGTCAGCCAGGGCAGGG NGG 2 0.0 Tier II
33 NC_000081.6 15 88249563 + 4930445N06Rik NNNAATCAGCCAGTGCAGAG NGG 1 0.7143 Tier III
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Other clones with same target sequence:

(none)