Construct: sgRNA BRDN0001149328
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATTCATACTATCCACGCCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ETNK2 (55224)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76167
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
204146723 |
- |
ETNK2 |
NNNCATACTATCCACGCCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
7109847 |
- |
GRM7 |
NNNCATACTATCCACAACAA |
NGG |
2 |
0.4667 |
Tier II |
| 3 |
NC_000023.11 |
X |
65417897 |
+ |
ZC3H12B |
NNNCATACTATCCACTCCAA |
NTG |
2 |
0.0 |
Tier II |
| 4 |
NC_000013.11 |
13 |
22045065 |
- |
LOC105370108 |
NNNCATACTGTCCACACCAA |
NGG |
2 |
0.5556 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
133368792 |
+ |
Etnk2 |
NNNCACACCATCCACGCCAA |
NGG |
2 |
0.8392 |
Tier I |
| 2 |
NC_000072.6 |
6 |
99246934 |
+ |
Foxp1 |
NNNCATACTATGCAAGCCAA |
NGG |
2 |
0.0988 |
Tier II |
| 3 |
NC_000077.6 |
11 |
120683915 |
+ |
Aspscr1 |
NNNCATACTATCCACCCCTA |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000072.6 |
6 |
70701735 |
- |
Igk |
NNNTATACTACCCACGCCAA |
NGG |
2 |
0.5333 |
Tier III |
Other clones with same target sequence:
(none)
