Construct: sgRNA BRDN0001149330

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCGCCGCCCGGTCATAGACG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GK5 (256356)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75775

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 142225352 + GK5 NNNCCGCCCGGTCATAGACG NGG 0 1.0 Tier I
2 NC_000006.12 6 33732687 - IP6K3 NNNCCGCCCTGTCATAGATG NGG 2 0.375 Tier II
3 NC_000022.11 22 21001562 + THAP7 NNNCCGCCCGGGCACAGACG NGG 2 0.1364 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 96119570 - Gk5 NNNCCGTCTGGTCATAGACG NGG 2 0.7109 Tier I
Download CSV

Other clones with same target sequence:

(none)