Construct: sgRNA BRDN0001149331

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTCATGTGCCAAGAAAGTGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IRAK4 (51135)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75666

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 43778208 - IRAK4 NNNATGTGCCAAGAAAGTGG NGG 0 1.0 Tier I
2 NC_000003.12 3 189306165 + TPRG1 NNNATGTGCTAAGAAAATGG NGG 2 0.8784 Tier II
3 NC_000005.10 5 111269171 + CAMK4 NNNATTTGCCAAGAAAATGG NGG 2 0.6222 Tier II
4 NC_000005.10 5 96870486 - ERAP1 NNNATGTTCTAAGAAAGTGG NGG 2 0.5882 Tier II
5 NC_000002.12 2 177809773 + PDE11A NNNGTGTGCCAAAAAAGTGG NGG 2 0.5769 Tier II
6 NC_000003.12 3 97172099 + EPHA6 NNNATGGGCCAAAAAAGTGG NGG 2 0.543 Tier II
7 NC_000013.11 13 43399934 - ENOX1 NNNATGAGGCAAGAAAGTGG NGG 2 0.5417 Tier II
8 NC_000007.14 7 50670918 - GRB10 NNNATGTGCCAGGAAAGTAG NGG 2 0.5159 Tier II
9 NC_000013.11 13 101070985 + NALCN NNNAAGTGCCAAGAAAATGG NGG 2 0.4667 Tier II
10 NC_000022.11 22 25895080 + MYO18B NNNAAGAGCCAAGAAAGTGG NGG 2 0.4375 Tier II
11 NC_000012.12 12 102931827 - PAH NNNATGTACCAACAAAGTGG NGG 2 0.4211 Tier II
12 NC_000002.12 2 209658414 + MAP2 NNNATGTGCAAACAAAGTGG NGG 2 0.3649 Tier II
13 NC_000003.12 3 69308525 - FRMD4B NNNATGAGCCCAGAAAGTGG NGG 2 0.35 Tier II
14 NC_000023.11 X 9930444 + SHROOM2 NNNATCTGCCAAGGAAGTGG NGG 2 0.3182 Tier II
15 NC_000003.12 3 157000966 - LEKR1 NNNATGTGCCAAGAAAAGGG NGG 2 0.3111 Tier II
16 NC_000012.12 12 55947387 + DGKA NNNATGTGCCAAGAAAGTGA NAG 2 0.2431 Tier II
17 NC_000008.11 8 28720951 - EXTL3 NNNAGGTGCCATGAAAGTGG NGG 2 0.2133 Tier II
18 NC_000012.12 12 16192162 + SLC15A5 NNNATATGCCAAGAAGGTGG NGG 2 0.1923 Tier II
19 NC_000007.14 7 43222105 - HECW1 NNNATGTGCCAAGACAGTTG NGG 2 0.1818 Tier II
20 NC_000012.12 12 44080337 + TMEM117 NNNAAGTGCCAAGCAAGTGG NGG 2 0.1071 Tier II
21 NC_000011.10 11 63887792 + MARK2 NNNATGTGCCCAGATAGTGG NGG 2 0.08 Tier II
22 NC_000003.12 3 63360611 - SYNPR NNNATGTGCCAAGCAAGGGG NGG 2 0.0714 Tier II
23 NC_000004.12 4 78206292 + FRAS1 NNNATGTGCCATGAAGGTGG NGG 2 0.0641 Tier II
24 NC_000016.10 16 25733209 + HS3ST4 NNNATGTGCCAAGAAATTGG NGA 2 0.0174 Tier II
25 NC_000019.10 19 7170867 - INSR NNNATGTGCCAAGCAAGTGG NGA 2 0.0149 Tier II
26 NC_000019.10 19 50023735 + VRK3 NNNATGTGCCAAGAAGGTGG NTG 2 0.0075 Tier II
27 NC_000023.11 X 155699421 + SPRY3 NNNATGTGCCAAGAACTTGG NGG 2 0.0 Tier II
28 NC_000009.12 9 25543785 + LOC105375996 NNNATGTACCAAGAAAGTTG NGG 2 0.6667 Tier III
29 NC_000002.12 2 51200329 + NRXN1-DT NNNATGTTCCAAGAAAGTGA NGG 2 0.5859 Tier III
30 NC_000011.10 11 90510164 + DISC1FP1 NNNATGTTCCAAGAAAGTGA NGG 2 0.5859 Tier III
31 NC_000022.11 22 25895080 + MYO18B-AS1 NNNAAGAGCCAAGAAAGTGG NGG 2 0.4375 Tier III
32 NC_000012.12 12 102931827 - LOC112267865 NNNATGTACCAACAAAGTGG NGG 2 0.4211 Tier III
33 NC_000012.12 12 75582168 - LOC105369844 NNNATGTGCCTAGAAAGTGG NGG 1 0.3077 Tier III
34 NC_000008.11 8 76531790 - LOC107986952 NNNATGTGCCCAGAAAGTAG NGG 2 0.2857 Tier III
35 NC_000012.12 12 16192162 + LOC101928362 NNNATATGCCAAGAAGGTGG NGG 2 0.1923 Tier III
36 NC_000020.11 20 60181107 - MIR646HG NNNATGTGCCAGGAAAGTGG NAG 2 0.1872 Tier III
37 NC_000020.11 20 7165410 + LINC01428 NNNATGTGCCAAGAAATTGG NAG 2 0.0648 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 77781148 + Gclc NNNATGTGCCAAGTAAGTGG NTG 2 0.0208 Tier I
2 NC_000085.6 19 29622915 - Ermp1 NNNATGTGCCAAGAAAATGT NGG 2 0.6533 Tier II
3 NC_000075.6 9 13480994 + Maml2 NNNATCTGCCAAGAAAGTGA NGG 2 0.6392 Tier II
4 NC_000068.7 2 121575852 - Frmd5 NNNATGTGTCAAGAAAGTGT NGG 2 0.6125 Tier II
5 NC_000072.6 6 50302582 - Osbpl3 NNNTTGTGTCAAGAAAGTGG NGG 2 0.5568 Tier II
6 NC_000085.6 19 31624213 + Prkg1 NNNATGGGCCAAGAAAGTGA NGG 2 0.5515 Tier II
7 NC_000084.6 18 38779987 - Arhgap26 NNNATGTGCCAAGAAAGAGT NGG 2 0.4667 Tier II
8 NC_000086.7 X 161993215 - Nhs NNNTTGTGCCAAGAAAGTAG NGG 2 0.4545 Tier II
9 NC_000078.6 12 36037366 - Tspan13 NNNATGTTCCAAGAAAGTAG NGG 2 0.4464 Tier II
10 NC_000067.6 1 104791977 + Cdh20 NNNAAGTGCCAAGAAAGAGG NGG 2 0.3333 Tier II
11 NC_000067.6 1 174750090 - Fmn2 NNNATGTGCTTAGAAAGTGG NGG 2 0.2896 Tier II
12 NC_000067.6 1 178412160 - Efcab2 NNNATGTGCCAAGAAACTGA NGG 2 0.2206 Tier II
13 NC_000074.6 8 106523299 + Cdh3 NNNATGTGGCTAGAAAGTGG NGG 2 0.1905 Tier II
14 NC_000080.6 14 23043712 + Lrmda NNNATGTGTCAAGAAGGTGG NGG 2 0.1683 Tier II
15 NC_000074.6 8 124942779 + Egln1 NNNATGTGCCAGGATAGTGG NGG 2 0.1444 Tier II
16 NC_000076.6 10 58949251 + Sh3rf3 NNNATGTGCCAAGAAAGGGC NGG 2 0.1429 Tier II
17 NC_000077.6 11 65864879 + Dnah9 NNNATGTGCAAAGAAAGTGG NGA 2 0.0602 Tier II
18 NC_000076.6 10 21016369 - Ahi1 NNNATGTGCCAAGAAAGAGG NGA 2 0.0463 Tier II
19 NC_000077.6 11 88552310 + Msi2 NNNATTTGCCAAGAAAGTGG NGA 2 0.0463 Tier II
20 NC_000067.6 1 96817082 + Slco4c1 NNNATGTGCCCAGAAAGTGG NGA 2 0.0278 Tier II
21 NC_000077.6 11 95032338 - Pdk2 NNNATGTGCCAAGAAAGGGG NGA 2 0.0231 Tier II
22 NC_000067.6 1 78192057 - Pax3 NNNATGGGCCAAGAATGTGG NGG 2 0.0 Tier II
23 NC_000084.6 18 69030091 - Gm31088 NNNATGAGACAAGAAAGTGG NGG 2 0.75 Tier III
24 NT_187064.1 34707 + LOC100861738 NNNGTGTGCCAAGAAAGTAG NGG 2 0.4464 Tier III
25 NT_187064.1 34707 + LOC100861749 NNNGTGTGCCAAGAAAGTAG NGG 2 0.4464 Tier III
26 NC_000068.7 2 122710923 + 4930417H01Rik NNNAAGTTCCAAGAAAGTGG NGG 2 0.3125 Tier III
27 NC_000067.6 1 174750090 - Gm51689 NNNATGTGCTTAGAAAGTGG NGG 2 0.2896 Tier III
28 NC_000070.6 4 52950725 + Gm33479 NNNATTTGCCAACAAAGTGG NGG 2 0.2807 Tier III
29 NC_000080.6 14 23043712 + Gm10248 NNNATGTGTCAAGAAGGTGG NGG 2 0.1683 Tier III
30 NC_000074.6 8 124942779 + Gm46060 NNNATGTGCCAGGATAGTGG NGG 2 0.1444 Tier III
31 NC_000079.6 13 48068098 - A330033J07Rik NNNATGTCCCAAGAAAGTGG NGA 2 0.0427 Tier III
32 NC_000077.6 11 95032338 - LOC115486429 NNNATGTGCCAAGAAAGGGG NGA 2 0.0231 Tier III
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Other clones with same target sequence:

(none)