Construct: sgRNA BRDN0001149332

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGAACAGAAATGTTGACCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RYK (6259)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76450

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 134211551 + RYK NNNACAGAAATGTTGACCTG NGG 0 1.0 Tier I
2 NC_000002.12 2 201754554 - ALS2 NNNACAGAAGTGTGGACCTG NGG 2 0.0 Tier I
3 NC_000002.12 2 209666205 + MAP2 NNNACACAAATGCTGACCTG NGG 2 0.5428 Tier II
4 NC_000001.11 1 147669510 + ACP6 NNNATAGAAATGCTGACCTG NGG 2 0.5024 Tier II
5 NC_000007.14 7 108418168 - NRCAM NNNACAGAAATGCAGACCTG NGG 2 0.4887 Tier II
6 NC_000005.10 5 21809889 + CDH12 NNNACAGAAATGTTGACATT NGG 2 0.3769 Tier II
7 NC_000017.11 17 74329556 + KIF19 NNNGGAGAAATGTTGACCTG NGG 2 0.375 Tier II
8 NC_000012.12 12 26974939 - TM7SF3 NNNTCAGAAATGTTGACATG NGG 2 0.3427 Tier II
9 NC_000002.12 2 218660867 + BCS1L NNNACAGTAATGCTGACCTG NGG 2 0.3383 Tier II
10 NC_000010.11 10 7625191 + ITIH5 NNNAGAGAAATGTTGACATG NGG 2 0.3231 Tier II
11 NC_000005.10 5 134577507 - JADE2 NNNACAGAAAGGATGACCTG NGG 2 0.2769 Tier II
12 NC_000002.12 2 214475787 + VWC2L NNNACAAAAATGTTGACCTG NAG 2 0.2593 Tier II
13 NC_000008.11 8 143522157 + ZC3H3 NNNACAGAACTGATGACCTG NGG 2 0.2308 Tier II
14 NC_000008.11 8 101994510 - NCALD NNNACAGAAGGGTTGACCTG NGG 2 0.2222 Tier II
15 NC_000002.12 2 191051133 + STAT4 NNNACACAAATGGTGACCTG NGG 2 0.1793 Tier II
16 NC_000007.14 7 7389350 - COL28A1 NNNACAGATATGTTGACCAG NGG 2 0.1714 Tier II
17 NC_000006.12 6 85457665 + NT5E NNNACAGAAATGATGGCCTG NGG 2 0.1331 Tier II
18 NC_000007.14 7 92651374 - CDK6 NNNACAGAAATGTTCACCTG NAG 2 0.0707 Tier II
19 NC_000022.11 22 40453830 - MRTFA NNNACAGAAATGTTTACCAG NGG 2 0.0408 Tier II
20 NC_000024.10 Y 22986306 + BPY2 NNNACAGAAATGTTGACCTA NTG 2 0.0365 Tier II
21 NC_000024.10 Y 24620047 + BPY2B NNNACAGAAATGTTGACCTA NTG 2 0.0365 Tier II
22 NC_000024.10 Y 25050062 - BPY2C NNNACAGAAATGTTGACCTA NTG 2 0.0365 Tier II
23 NC_000023.11 X 1317640 - CSF2RA NNNACAGAAATGGTGACCTG NGA 2 0.0181 Tier II
24 NC_000024.10 Y 1317640 - CSF2RA NNNACAGAAATGGTGACCTG NGA 2 0.0181 Tier II
25 NC_000017.11 17 54945511 + TOM1L1 NNNACACAAATGTTGACCTG NGC 2 0.0153 Tier II
26 NC_000006.12 6 44866071 + SUPT3H NNNACAGAAATGTTGAGGTG NGG 2 0.0078 Tier II
27 NC_000011.10 11 94064599 + HEPHL1 NNNACAGAAATGTTGATCTG NGT 2 0.0075 Tier II
28 NC_000014.9 14 89248550 + FOXN3 NNNACAGAAATGATGCCCTG NGG 2 0.0 Tier II
29 NC_000007.14 7 48583413 + ABCA13 NNNACAGAAATGTTGACCTG NCC 2 0.0 Tier II
30 NC_000017.11 17 4222490 - ANKFY1 NNNACAGAAATGTTGCCCTG NGG 1 0.0 Tier II
31 NC_000023.11 X 11849578 + FRMPD4 NNNACAGACATGTGGACCTG NGG 2 0.0 Tier II
32 NC_000004.12 4 47416854 - GABRB1 NNNCCAGAAATGTTGCCCTG NGG 2 0.0 Tier II
33 NC_000004.12 4 174368136 + LOC105377547 NNNACAGAATTCTTGACCTG NGG 2 0.4671 Tier III
34 NC_000007.14 7 100602239 - PCOLCE-AS1 NNNACATAAATGTTGACCTT NGG 2 0.4 Tier III
35 NC_000008.11 8 16870008 - LOC105379297 NNNTCAGATATGTTGACCTG NGG 2 0.3818 Tier III
36 NC_000023.11 X 128428880 + LOC107985698 NNNACAGAAATGTTGACATT NGG 2 0.3769 Tier III
37 NC_000008.11 8 101994510 - LOC104054148 NNNACAGAAGGGTTGACCTG NGG 2 0.2222 Tier III
38 NC_000010.11 10 28449231 - LINC02652 NNNACAGAAAGGTTGGCCTG NGG 2 0.0769 Tier III
39 NC_000007.14 7 92651374 - LOC112268009 NNNACAGAAATGTTCACCTG NAG 2 0.0707 Tier III
40 NC_000020.11 20 52579063 + LOC105372666 NNNACAGAAATGTTAAGCTG NGG 2 0.0554 Tier III
41 NC_000018.10 18 79031785 - LOC105372225 NNNACAGAAATGTTGACCTT NGT 2 0.0113 Tier III
42 NC_000017.11 17 4222490 - RYKP1 NNNACAGAAATGTTGCCCTG NGG 1 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 102867612 - Ryk NNNGCAGAAATATTGACCTG NGG 2 0.5833 Tier I
2 NC_000069.6 3 27890842 + Tmem212 NNNACAAGAATGTTGACCTG NGG 2 0.7333 Tier II
3 NC_000076.6 10 49220830 - Grik2 NNNACAGAAATATTGACATG NGG 2 0.5026 Tier II
4 NC_000075.6 9 73492653 + Unc13c NNNATGGAAATGTTGACCTG NGG 2 0.4545 Tier II
5 NC_000085.6 19 55421528 - Vti1a NNNACAGAAATGTTAAACTG NGG 2 0.4392 Tier II
6 NC_000068.7 2 162300635 + Ptprt NNNAAAGAAATGTAGACCTG NGG 2 0.3537 Tier II
7 NC_000082.6 16 97001715 + Dscam NNNACAGACATGTAGACCTG NGG 2 0.3537 Tier II
8 NC_000071.6 5 102044431 + Wdfy3 NNNAGAGAAATGTTGACATG NGG 2 0.3231 Tier II
9 NC_000072.6 6 56174315 - Pde1c NNNACACAAATGTTGATCTG NGG 2 0.3208 Tier II
10 NC_000071.6 5 141657936 - Sdk1 NNNACACAAATGTTGACCTC NGG 2 0.2946 Tier II
11 NC_000084.6 18 77692607 + 8030462N17Rik NNNACAGAAATGTAGATCTG NGG 2 0.2889 Tier II
12 NC_000067.6 1 146642306 - Brinp3 NNNACAGAAATGCTCACCTG NGG 2 0.2153 Tier II
13 NC_000080.6 14 12143192 - Ptprg NNNACAGAAATGTTGGCCTG NGG 1 0.1923 Tier II
14 NC_000077.6 11 47364859 - Sgcd NNNCCAGAAATGTTGACATG NGG 2 0.19 Tier II
15 NC_000070.6 4 144981977 + Vps13d NNNACACAAATGTTGACCTG NAG 2 0.1782 Tier II
16 NC_000081.6 15 33573513 - Cpq NNNACAGATATGTTGACCAG NGG 2 0.1714 Tier II
17 NC_000067.6 1 195109860 + Cr1l NNNACAGACATGTTGACCAG NGG 2 0.1633 Tier II
18 NC_000070.6 4 94645951 + Ift74 NNNACAGAAATGTTGATCAG NGG 2 0.1333 Tier II
19 NC_000070.6 4 94645951 + Lrrc19 NNNACAGAAATGTTGATCAG NGG 2 0.1333 Tier II
20 NC_000083.6 17 75371101 + Ltbp1 NNNACAGAAATGTTGACCTG NGA 1 0.0694 Tier II
21 NC_000073.6 7 128746827 - Sec23ip NNNACAGAAATGTTGAGCTG NAG 2 0.0153 Tier II
22 NC_000070.6 4 113763008 + Skint5 NNNACAGAAATGTTAACCTG NGT 2 0.0152 Tier II
23 NC_000068.7 2 73885553 + Atf2 NNNACAGAAATGATGACCTG NGT 2 0.0112 Tier II
24 NC_000072.6 6 98247610 - Gm765 NNNACAGAAATGTGGAACTG NGG 2 0.0 Tier II
25 NC_000076.6 10 87906379 - Igf1 NNNACAGAACTGTTGTCCTG NGG 2 0.0 Tier II
26 NC_000081.6 15 27522198 - Ank NNNACAGAGATGTTGCCCTG NGG 2 0.0 Tier II
27 NC_000085.6 19 38761974 + Plce1 NNNACTGAAATGTGGACCTG NGG 2 0.0 Tier II
28 NC_000083.6 17 83868895 + Gm35125 NNNACAGCAATGCTGACCTG NGG 2 0.3383 Tier III
29 NC_000073.6 7 25551278 + 4732471J01Rik NNNACAGAAATGTAGAGCTG NGG 2 0.0364 Tier III
30 NC_000072.6 6 92983922 - 9530026P05Rik NNNACAGAAATGAGGACCTG NGG 2 0.0 Tier III
31 NC_000072.6 6 98247610 - Gm32592 NNNACAGAAATGTGGAACTG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)