Construct: sgRNA BRDN0001149340

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACAATGTAAAAGCTACCCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK5 (26289)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76552

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 77340491 + AK5 NNNATGTAAAAGCTACCCAA NGG 0 1.0 Tier I
2 NC_000003.12 3 45433412 - LARS2 NNNATAAAAAAGCTACCCAA NGG 2 0.875 Tier II
3 NC_000012.12 12 100501256 - NR1H4 NNNGTGAAAAAGCTACCCAA NGG 2 0.5469 Tier II
4 NC_000003.12 3 135189578 - EPHB1 NNNATTTAAAAGCTGCCCAA NGG 2 0.4333 Tier II
5 NC_000015.10 15 67789969 - MAP2K5 NNNGTGTAAAAGCTGCCCAA NGG 2 0.4063 Tier II
6 NC_000023.11 X 119109237 + KIAA1210 NNNTTGTAAAAGCAACCCAA NGG 2 0.3939 Tier II
7 NC_000005.10 5 36672714 + SLC1A3 NNNATGTAAACACTACCCAA NGG 2 0.3733 Tier II
8 NC_000018.10 18 2760068 + SMCHD1 NNNATGCAAAAGCTACCCTA NGG 2 0.3702 Tier II
9 NC_000004.12 4 139466493 - RAB33B NNNATATAAAAGCTATCCAA NGG 2 0.3077 Tier II
10 NC_000006.12 6 46447164 + RCAN2 NNNATGTAAATGCTAACCAA NGG 2 0.3077 Tier II
11 NC_000011.10 11 10635167 - IRAG1 NNNATGTCAAAGTTACCCAA NGG 2 0.3 Tier II
12 NC_000004.12 4 172355712 - GALNTL6 NNNATGTAAAAGCTACTCAT NGG 2 0.28 Tier II
13 NC_000001.11 1 197735206 - DENND1B NNNATGTAAAAGATACTCAA NGG 2 0.1795 Tier II
14 NC_000006.12 6 42406827 + TRERF1 NNNATGTACAAGCTCCCCAA NGG 2 0.1558 Tier II
15 NC_000006.12 6 35067850 - ANKS1A NNNATGTAAAAGCTACCCAT NAG 2 0.1556 Tier II
16 NC_000002.12 2 69775398 - ANXA4 NNNAGGTAAAAGCTACCCCA NGG 2 0.1324 Tier II
17 NC_000001.11 1 182854501 + DHX9 NNNATGTAAAAGCCAGCCAA NGG 2 0.044 Tier II
18 NC_000012.12 12 62086884 + TAFA2 NNNATGTAAAAGCTACCCAA NTG 1 0.039 Tier II
19 NC_000001.11 1 245172999 + KIF26B NNNATGTAGAAGCTACCCAA NTG 2 0.026 Tier II
20 NC_000003.12 3 57811510 - SLMAP NNNATGTAAAAGCAACCCAA NTG 2 0.0241 Tier II
21 NC_000003.12 3 51244287 - DOCK3 NNNATGTAAATGCTACCCAA NGT 2 0.005 Tier II
22 NC_000001.11 1 8911988 - LOC112268261 NNNATATAAAACCTACCCAA NGG 2 0.5294 Tier III
23 NC_000005.10 5 36672714 + SLC1A3-AS1 NNNATGTAAACACTACCCAA NGG 2 0.3733 Tier III
24 NC_000002.12 2 100227182 + LINC01104 NNNATGTAAAAGGTAACCAA NGG 2 0.1364 Tier III
25 NC_000017.11 17 69643892 - LINC01483 NNNCTGTAAAAGCTCCCCAA NGG 2 0.0963 Tier III
26 NC_000001.11 1 56291770 + LOC124904186 NNNATGTAACAGCTTCCCAA NGG 2 0.0667 Tier III
27 NC_000004.12 4 114741540 + LOC105377382 NNNATGTCAAAGGTACCCAA NGG 2 0.0584 Tier III
28 NC_000023.11 X 51226839 + LOC105373204 NNNATGTAAAAGGTATCCAA NGG 2 0.042 Tier III
29 NC_000018.10 18 73458181 + LOC105372190 NNNATGTAAAATCTACCCAA NGA 2 0.0267 Tier III
30 NC_000007.14 7 55586716 - VOPP1-DT NNNATGTAAAACCTACCCAA NTG 2 0.0206 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 108942660 + Axin2 NNNATGCAAAAGCCACCCAA NGG 2 0.1964 Tier I
2 NC_000067.6 1 165872548 + Pou2f1 NNNAAGTAAAAACTACCCAA NGG 2 0.4667 Tier II
3 NC_000068.7 2 156515894 + Epb41l1 NNNATGGAGAAGCTACCCAA NGG 2 0.3922 Tier II
4 NC_000078.6 12 95699519 - Flrt2 NNNATGTAAAACCTGCCCAA NGG 2 0.3441 Tier II
5 NC_000068.7 2 72297680 - Map3k20 NNNAAGGAAAAGCTACCCAA NGG 2 0.2941 Tier II
6 NC_000071.6 5 136517452 + Cux1 NNNATGTCAAAGCTACCTAA NGG 2 0.2755 Tier II
7 NC_000067.6 1 58525769 + Fam126b NNNATGTAAAAGCTACTAAA NGG 2 0.2513 Tier II
8 NC_000080.6 14 86828541 + Diaph3 NNNATGTAAAACCTACTCAA NGG 2 0.2471 Tier II
9 NC_000070.6 4 116356841 + Mast2 NNNATGTAACAGTTACCCAA NGG 2 0.2333 Tier II
10 NC_000068.7 2 31508044 - Ass1 NNNATGTAAAAGCTACCCCA NGG 1 0.2069 Tier II
11 NC_000074.6 8 111586811 - Znrf1 NNNATGGAAAAGCTCCCCAA NGG 2 0.1604 Tier II
12 NC_000085.6 19 31124696 + Prkg1 NNNATGTACAAGCTACCCAA NAG 2 0.1481 Tier II
13 NC_000081.6 15 66533287 + Tmem71 NNNATGTAAAAGCCACTCAA NGG 2 0.1333 Tier II
14 NC_000079.6 13 56785268 - Trpc7 NNNATGTAAAACCTTCCCAA NGG 2 0.1059 Tier II
15 NC_000071.6 5 71042327 + Gabra2 NNNATGTCAAAGCTTCCCAA NGG 2 0.0857 Tier II
16 NC_000068.7 2 125328379 + Fbn1 NNNCTGTAAAAGCTTCCCAA NGG 2 0.0706 Tier II
17 NC_000069.6 3 32580937 - Gnb4 NNNATGTAAAAGCCACCCAC NGG 2 0.0649 Tier II
18 NC_000072.6 6 94063879 + Magi1 NNNATGTAAAAGCTATCCCA NGG 2 0.0637 Tier II
19 NC_000072.6 6 11938025 - Phf14 NNNATGTAAAAGCTAAGCAA NGG 2 0.0588 Tier II
20 NC_000080.6 14 10063359 + Fhit NNNATGTAAATGCTAGCCAA NGG 2 0.0473 Tier II
21 NC_000072.6 6 97545255 - Frmd4b NNNATGTAAAAGCTGCGCAA NGG 2 0.0382 Tier II
22 NC_000070.6 4 11345660 + Esrp1 NNNATGTAAAAGCAACCCAA NTG 2 0.0241 Tier II
23 NC_000074.6 8 32329495 - Nrg1 NNNATGTAAAGGCTACCCAA NGT 2 0.0105 Tier II
24 NC_000082.6 16 34533580 + Kalrn NNNATGTAAAAGCTTCCCAA NGT 2 0.0032 Tier II
25 NC_000074.6 8 35242191 + Gm34597 NNNATGAAAAAGCTACCCAG NGG 2 0.6691 Tier III
26 NC_000067.6 1 58525769 + Gm15834 NNNATGTAAAAGCTACTAAA NGG 2 0.2513 Tier III
27 NC_000070.6 4 124056716 - Gm40241 NNNATATAAAAGCTACCCCA NGG 2 0.2069 Tier III
28 NC_000077.6 11 45095877 - Gm51859 NNNATGTAAAAGCTAACCCA NGG 2 0.2069 Tier III
29 NC_000081.6 15 70023863 - Gm19782 NNNATGTAAAATGTACCCAA NGG 2 0.0524 Tier III
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Other clones with same target sequence:

(none)