Construct: sgRNA BRDN0001149340
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAATGTAAAAGCTACCCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AK5 (26289)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76552
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000077.6 |
11 |
108942660 |
+ |
Axin2 |
NNNATGCAAAAGCCACCCAA |
NGG |
2 |
0.1964 |
Tier I |
2 |
NC_000067.6 |
1 |
165872548 |
+ |
Pou2f1 |
NNNAAGTAAAAACTACCCAA |
NGG |
2 |
0.4667 |
Tier II |
3 |
NC_000068.7 |
2 |
156515894 |
+ |
Epb41l1 |
NNNATGGAGAAGCTACCCAA |
NGG |
2 |
0.3922 |
Tier II |
4 |
NC_000078.6 |
12 |
95699519 |
- |
Flrt2 |
NNNATGTAAAACCTGCCCAA |
NGG |
2 |
0.3441 |
Tier II |
5 |
NC_000068.7 |
2 |
72297680 |
- |
Map3k20 |
NNNAAGGAAAAGCTACCCAA |
NGG |
2 |
0.2941 |
Tier II |
6 |
NC_000071.6 |
5 |
136517452 |
+ |
Cux1 |
NNNATGTCAAAGCTACCTAA |
NGG |
2 |
0.2755 |
Tier II |
7 |
NC_000067.6 |
1 |
58525769 |
+ |
Fam126b |
NNNATGTAAAAGCTACTAAA |
NGG |
2 |
0.2513 |
Tier II |
8 |
NC_000080.6 |
14 |
86828541 |
+ |
Diaph3 |
NNNATGTAAAACCTACTCAA |
NGG |
2 |
0.2471 |
Tier II |
9 |
NC_000070.6 |
4 |
116356841 |
+ |
Mast2 |
NNNATGTAACAGTTACCCAA |
NGG |
2 |
0.2333 |
Tier II |
10 |
NC_000068.7 |
2 |
31508044 |
- |
Ass1 |
NNNATGTAAAAGCTACCCCA |
NGG |
1 |
0.2069 |
Tier II |
11 |
NC_000074.6 |
8 |
111586811 |
- |
Znrf1 |
NNNATGGAAAAGCTCCCCAA |
NGG |
2 |
0.1604 |
Tier II |
12 |
NC_000085.6 |
19 |
31124696 |
+ |
Prkg1 |
NNNATGTACAAGCTACCCAA |
NAG |
2 |
0.1481 |
Tier II |
13 |
NC_000081.6 |
15 |
66533287 |
+ |
Tmem71 |
NNNATGTAAAAGCCACTCAA |
NGG |
2 |
0.1333 |
Tier II |
14 |
NC_000079.6 |
13 |
56785268 |
- |
Trpc7 |
NNNATGTAAAACCTTCCCAA |
NGG |
2 |
0.1059 |
Tier II |
15 |
NC_000071.6 |
5 |
71042327 |
+ |
Gabra2 |
NNNATGTCAAAGCTTCCCAA |
NGG |
2 |
0.0857 |
Tier II |
16 |
NC_000068.7 |
2 |
125328379 |
+ |
Fbn1 |
NNNCTGTAAAAGCTTCCCAA |
NGG |
2 |
0.0706 |
Tier II |
17 |
NC_000069.6 |
3 |
32580937 |
- |
Gnb4 |
NNNATGTAAAAGCCACCCAC |
NGG |
2 |
0.0649 |
Tier II |
18 |
NC_000072.6 |
6 |
94063879 |
+ |
Magi1 |
NNNATGTAAAAGCTATCCCA |
NGG |
2 |
0.0637 |
Tier II |
19 |
NC_000072.6 |
6 |
11938025 |
- |
Phf14 |
NNNATGTAAAAGCTAAGCAA |
NGG |
2 |
0.0588 |
Tier II |
20 |
NC_000080.6 |
14 |
10063359 |
+ |
Fhit |
NNNATGTAAATGCTAGCCAA |
NGG |
2 |
0.0473 |
Tier II |
21 |
NC_000072.6 |
6 |
97545255 |
- |
Frmd4b |
NNNATGTAAAAGCTGCGCAA |
NGG |
2 |
0.0382 |
Tier II |
22 |
NC_000070.6 |
4 |
11345660 |
+ |
Esrp1 |
NNNATGTAAAAGCAACCCAA |
NTG |
2 |
0.0241 |
Tier II |
23 |
NC_000074.6 |
8 |
32329495 |
- |
Nrg1 |
NNNATGTAAAGGCTACCCAA |
NGT |
2 |
0.0105 |
Tier II |
24 |
NC_000082.6 |
16 |
34533580 |
+ |
Kalrn |
NNNATGTAAAAGCTTCCCAA |
NGT |
2 |
0.0032 |
Tier II |
25 |
NC_000074.6 |
8 |
35242191 |
+ |
Gm34597 |
NNNATGAAAAAGCTACCCAG |
NGG |
2 |
0.6691 |
Tier III |
26 |
NC_000067.6 |
1 |
58525769 |
+ |
Gm15834 |
NNNATGTAAAAGCTACTAAA |
NGG |
2 |
0.2513 |
Tier III |
27 |
NC_000070.6 |
4 |
124056716 |
- |
Gm40241 |
NNNATATAAAAGCTACCCCA |
NGG |
2 |
0.2069 |
Tier III |
28 |
NC_000077.6 |
11 |
45095877 |
- |
Gm51859 |
NNNATGTAAAAGCTAACCCA |
NGG |
2 |
0.2069 |
Tier III |
29 |
NC_000081.6 |
15 |
70023863 |
- |
Gm19782 |
NNNATGTAAAATGTACCCAA |
NGG |
2 |
0.0524 |
Tier III |
Other clones with same target sequence:
(none)