Construct: sgRNA BRDN0001149343
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGAGTATACAGCATCGAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKAG2 (51422)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77462
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
151568837 |
+ |
PRKAG2 |
NNNAGTATACAGCATCGAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
63511858 |
+ |
LGALS12 |
NNNAGTATCCAGCATCTAAG |
NGG |
2 |
0.1429 |
Tier II |
| 3 |
NC_000009.12 |
9 |
1474129 |
- |
LOC102723803 |
NNNAGTATACAGTATCGAAG |
NGG |
1 |
0.7 |
Tier III |
| 4 |
NC_000016.10 |
16 |
57594190 |
- |
LOC105371291 |
NNNAGTATACAGCATTGAAA |
NGG |
2 |
0.2885 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
24874005 |
+ |
Prkag2 |
NNNAGTATACAGCATCGAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000082.6 |
16 |
20720566 |
+ |
Polr2h |
NNNAGTGTACAGGATCGAAG |
NGG |
2 |
0.0963 |
Tier I |
| 3 |
NC_000074.6 |
8 |
38316130 |
+ |
Sgcz |
NNNAGTAAACAACATCGAAG |
NGG |
2 |
0.7467 |
Tier II |
| 4 |
NC_000076.6 |
10 |
28567193 |
+ |
Ptprk |
NNNAGTATACAGCATCCAAA |
NGG |
2 |
0.2206 |
Tier II |
| 5 |
NC_000080.6 |
14 |
77396081 |
+ |
Enox1 |
NNNAATATACAGCATCTAAG |
NGG |
2 |
0.2167 |
Tier II |
| 6 |
NC_000078.6 |
12 |
55067904 |
- |
2700097O09Rik |
NNNAGTATACAGGATCGAAG |
NGG |
1 |
0.1364 |
Tier II |
| 7 |
NC_000081.6 |
15 |
71638458 |
+ |
Fam135b |
NNNAGTATACAGCATCAAAG |
NTG |
2 |
0.0364 |
Tier II |
| 8 |
NC_000068.7 |
2 |
139268158 |
- |
Gm14066 |
NNNAGTATACAGCATCGAGT |
NGG |
2 |
0.2625 |
Tier III |
| 9 |
NC_000078.6 |
12 |
55067904 |
- |
Gm7511 |
NNNAGTATACAGGATCGAAG |
NGG |
1 |
0.1364 |
Tier III |
Other clones with same target sequence:
(none)
