Construct: sgRNA BRDN0001149348
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGGAGACATCTATCTCGGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK1D (1453)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76716
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
82273306 |
- |
CSNK1D |
NNNAGACATCTATCTCGGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
25761464 |
+ |
SLC17A1 |
NNNAGACATCTAACTCGGTG |
NAG |
2 |
0.1795 |
Tier II |
| 3 |
NC_000006.12 |
6 |
25761464 |
+ |
SLC17A4 |
NNNAGACATCTAACTCGGTG |
NAG |
2 |
0.1795 |
Tier II |
| 4 |
NC_000003.12 |
3 |
79252893 |
- |
ROBO1 |
NNNAGACATCTATCTCCTTG |
NGG |
2 |
0.1569 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
120990935 |
- |
Csnk1d |
NNNAGACATCTATCTCGGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000082.6 |
16 |
17842454 |
- |
Dgcr2 |
NNNAGACACCTATCTCAGTG |
NGG |
2 |
0.8615 |
Tier II |
| 3 |
NC_000067.6 |
1 |
116009816 |
+ |
Cntnap5a |
NNNAGAGATCTATCTCAGTG |
NGG |
2 |
0.4392 |
Tier II |
| 4 |
NC_000074.6 |
8 |
46489431 |
- |
Acsl1 |
NNNAGACATGTGTCTCGGTG |
NGG |
2 |
0.2809 |
Tier II |
| 5 |
NC_000083.6 |
17 |
28550060 |
+ |
Clpsl2 |
NNNAGACATCTATCTCAGTG |
NAG |
2 |
0.242 |
Tier II |
| 6 |
NC_000077.6 |
11 |
84293204 |
- |
Acaca |
NNNAGACATCTATCTCTCTG |
NGG |
2 |
0.119 |
Tier II |
| 7 |
NC_000083.6 |
17 |
72172476 |
- |
Alk |
NNNAGACATCTCTCTGGGTG |
NGG |
2 |
0.0405 |
Tier II |
| 8 |
NC_000070.6 |
4 |
115049518 |
- |
Gm52674 |
NNNAGACATCTATCTCTGTG |
NAG |
2 |
0.0648 |
Tier III |
| 9 |
NC_000069.6 |
3 |
82885769 |
+ |
Rbm46os |
NNNAGACATCTATCTCAGTG |
NTG |
2 |
0.0364 |
Tier III |
Other clones with same target sequence:
(none)
