Construct: sgRNA BRDN0001149349
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCATCAACAGAGCCCGCCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- WEE1 (7465)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77367
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
9576643 |
- |
WEE1 |
NNNTCAACAGAGCCCGCCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
45965902 |
+ |
WNT7B |
NNNGCAACAGAGCCCGCCCA |
NGG |
2 |
0.1293 |
Tier II |
| 3 |
NC_000015.10 |
15 |
74008429 |
- |
PML |
NNNTCAACAGAGCCCTCCAC |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000004.12 |
4 |
55031359 |
+ |
LOC124900703 |
NNNTCAACAGAGCCTGCCAA |
NAG |
2 |
0.0173 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
110126152 |
- |
Wee1 |
NNNTCAACAGAGCCAGCCAA |
NGG |
1 |
0.2222 |
Tier I |
| 2 |
NC_000068.7 |
2 |
68978086 |
- |
Cers6 |
NNNTCACCAGAGCCCACCAA |
NGG |
2 |
0.4375 |
Tier II |
| 3 |
NC_000072.6 |
6 |
110647873 |
+ |
Grm7 |
NNNTCAACAGAGCACACCAA |
NGG |
2 |
0.35 |
Tier II |
| 4 |
NC_000071.6 |
5 |
9680637 |
- |
Grm3 |
NNNTCAACAGTGCCAGCCAA |
NGG |
2 |
0.0684 |
Tier II |
| 5 |
NC_000075.6 |
9 |
62466212 |
+ |
Coro2b |
NNNTCAACAGAGCCAGGCAA |
NGG |
2 |
0.0131 |
Tier II |
| 6 |
NC_000081.6 |
15 |
84294573 |
- |
Parvb |
NNNTCAACAGAGCCTGCCAA |
NGA |
2 |
0.0046 |
Tier II |
| 7 |
NC_000082.6 |
16 |
40439078 |
- |
Lsamp |
NNNTCAACAGAGCCAGCCAA |
NGT |
2 |
0.0036 |
Tier II |
| 8 |
NC_000067.6 |
1 |
97945544 |
+ |
Pam |
NNNTCAACAGAGCCCGGCAA |
NGT |
2 |
0.0009 |
Tier II |
| 9 |
NC_000072.6 |
6 |
48033720 |
+ |
Zfp777 |
NNNTCAACAGAGCCCCCCAA |
NTG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
