Construct: sgRNA BRDN0001149352
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGTGGGGCTTCCGTAAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCA (5578)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75925
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
66641390 |
- |
PRKCA |
NNNTGGGGCTTCCGTAAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
17245628 |
+ |
TBC1D5 |
NNNTGGGGCTTACGTAATTG |
NGG |
2 |
0.4762 |
Tier II |
| 3 |
NC_000001.11 |
1 |
233230329 |
+ |
PCNX2 |
NNNAGGGGCTTGCGTAAGTG |
NGG |
2 |
0.2116 |
Tier II |
| 4 |
NC_000011.10 |
11 |
45967448 |
+ |
PHF21A |
NNNTTGGGCTTCCCTAAGTG |
NGG |
2 |
0.1286 |
Tier II |
| 5 |
NC_000006.12 |
6 |
157998327 |
- |
SYNJ2 |
NNNTGGGGCTTCCTTAGGTG |
NGG |
2 |
0.0471 |
Tier II |
| 6 |
NC_000021.9 |
21 |
39667808 |
- |
B3GALT5 |
NNNTGGGGCCTCCGTAAGTG |
NGT |
2 |
0.0086 |
Tier II |
| 7 |
NC_000006.12 |
6 |
165938616 |
+ |
PDE10A |
NNNTGGGGCTTCCTTCAGTG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000015.10 |
15 |
30214029 |
+ |
LINC02249 |
NNNTGGGGCTTCCATAAGAG |
NGG |
2 |
0.2143 |
Tier III |
| 9 |
NC_000015.10 |
15 |
32324656 |
- |
LOC102724078 |
NNNTGGGGCTTCCATAAGAG |
NGG |
2 |
0.2143 |
Tier III |
| 10 |
NC_000007.14 |
7 |
26015041 |
- |
LOC105375199 |
NNNTGGGGCCTCCTTAAGTG |
NGG |
2 |
0.1422 |
Tier III |
| 11 |
NC_000002.12 |
2 |
84282301 |
+ |
LOC107985905 |
NNNTGGGGCTTAGGTAAGTG |
NGG |
2 |
0.0974 |
Tier III |
| 12 |
NC_000010.11 |
10 |
31989215 |
- |
LOC105376485 |
NNNTGTGGCTTCCGTAAGTG |
NGA |
2 |
0.0463 |
Tier III |
| 13 |
NC_000010.11 |
10 |
31989215 |
- |
LOC107984219 |
NNNTGTGGCTTCCGTAAGTG |
NGA |
2 |
0.0463 |
Tier III |
| 14 |
NC_000006.12 |
6 |
165938616 |
+ |
LINC00473 |
NNNTGGGGCTTCCTTCAGTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
108057839 |
+ |
Prkca |
NNNTAGGGCTTCCGTATGTG |
NGG |
2 |
0.1156 |
Tier I |
| 2 |
NC_000072.6 |
6 |
55347385 |
+ |
Aqp1 |
NNNTGGGGATTCTGTAAGTG |
NGG |
2 |
0.6 |
Tier II |
| 3 |
NC_000068.7 |
2 |
166677981 |
+ |
Prex1 |
NNNTGTGGTTTCCGTAAGTG |
NGG |
2 |
0.5833 |
Tier II |
| 4 |
NC_000071.6 |
5 |
130320689 |
- |
Tyw1 |
NNNTGTGGCTTCCATAAGTG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000083.6 |
17 |
8843590 |
+ |
Pde10a |
NNNTGGGGCTTCCAAAAGTG |
NGG |
2 |
0.4342 |
Tier II |
| 6 |
NC_000068.7 |
2 |
110166979 |
- |
Ccdc34 |
NNNTGGGGGTTCTGTAAGTG |
NGG |
2 |
0.4333 |
Tier II |
| 7 |
NC_000067.6 |
1 |
186707267 |
+ |
Tgfb2 |
NNNCGGGGGTTCCGTAAGTG |
NGG |
2 |
0.4006 |
Tier II |
| 8 |
NC_000074.6 |
8 |
14845972 |
+ |
Dlgap2 |
NNNTGGTGCTTCAGTAAGTG |
NGG |
2 |
0.2198 |
Tier II |
| 9 |
NC_000067.6 |
1 |
170655194 |
+ |
Olfml2b |
NNNTGTGGCTTCCTTAAGTG |
NGG |
2 |
0.1778 |
Tier II |
| 10 |
NC_000077.6 |
11 |
103217924 |
- |
Spata32 |
NNNTGGGGCTTCCGGAAGAG |
NGG |
2 |
0.0143 |
Tier II |
| 11 |
NC_000080.6 |
14 |
31310732 |
- |
Dnah1 |
NNNTGGGGCTTCCGTTACTG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000083.6 |
17 |
45737671 |
+ |
F630040K05Rik |
NNNTGGGGCTTCCCTAAGTG |
NGG |
1 |
0.4286 |
Tier III |
| 13 |
NC_000084.6 |
18 |
38096463 |
- |
Gm30093 |
NNNTGGGGCTTCCGCAAGTG |
NGA |
2 |
0.0189 |
Tier III |
| 14 |
NC_000080.6 |
14 |
31310732 |
- |
Gm35823 |
NNNTGGGGCTTCCGTTACTG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
