Construct: sgRNA BRDN0001149354
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCGCGCGTAGAAGTCCACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDK1 (5163)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77011
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
172556288 |
- |
PDK1 |
NNNGCGCGTAGAAGTCCACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
97854880 |
- |
FOXE1 |
NNNGCCCGTAGAAGTCCACC |
NTG |
2 |
0.0266 |
Tier I |
| 3 |
NC_000016.10 |
16 |
66802027 |
- |
TERB1 |
NNNGCGCGGAGAAGTCCTCC |
NGG |
2 |
0.3095 |
Tier II |
| 4 |
NC_000023.11 |
X |
37615202 |
+ |
LANCL3 |
NNNGCGCGTAGAAGTCCCCA |
NGG |
2 |
0.0952 |
Tier II |
| 5 |
NC_000002.12 |
2 |
172556288 |
- |
PDK1-AS1 |
NNNGCGCGTAGAAGTCCACC |
NGG |
0 |
1.0 |
Tier III |
| 6 |
NC_000015.10 |
15 |
34520697 |
- |
DNM1P5 |
NNNCCGCGTAGGAGTCCACC |
NGG |
2 |
0.3824 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
71873507 |
- |
Pdk1 |
NNNGCGCATAGAAGTCCACC |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000070.6 |
4 |
46345150 |
- |
Foxe1 |
NNNGCCCGTAGAAGTCCACC |
NTG |
2 |
0.0266 |
Tier I |
Other clones with same target sequence:
(none)
