Construct: sgRNA BRDN0001149355

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGACAACAGCAATATCCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K3 (4215)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76017

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 63657877 - MAP3K3 NNNACAACAGCAATATCCTA NGG 0 1.0 Tier I
2 NC_000002.12 2 207574710 - CREB1 NNNACAACTGAAATATCCTA NGG 2 0.45 Tier II
3 NC_000011.10 11 40836628 - LRRC4C NNNACAACAGCAAAACCCTA NGG 2 0.4127 Tier II
4 NC_000011.10 11 57424634 + SLC43A3 NNNAAAAAAGCAATATCCTA NGG 2 0.3714 Tier II
5 NC_000007.14 7 10980900 - PHF14 NNNACAAGAGCAATATCATA NGG 2 0.3462 Tier II
6 NC_000009.12 9 70946477 + TRPM3 NNNACAGCAGCAATATTCTA NGG 2 0.3294 Tier II
7 NC_000004.12 4 99923683 + DNAJB14 NNNACAACAGCAGTATTCTA NGG 2 0.3043 Tier II
8 NC_000003.12 3 58170877 - FLNB NNNACAACAGCAATATTTTA NGG 2 0.3 Tier II
9 NC_000015.10 15 44081947 + FRMD5 NNNTCAACACCAATATCCTA NGG 2 0.2545 Tier II
10 NC_000006.12 6 70021670 + COL19A1 NNNACAACAGCAATGTCCAA NGG 2 0.1857 Tier II
11 NC_000014.9 14 99854911 - EML1 NNNACAACAGCAATATCTAA NGG 2 0.1837 Tier II
12 NC_000021.9 21 26488800 + CYYR1 NNNACAAGAGCCATATCCTA NGG 2 0.1692 Tier II
13 NC_000009.12 9 109866292 - PALM2AKAP2 NNNACAACAGCCAAATCCTA NGG 2 0.1629 Tier II
14 NC_000017.11 17 28387070 + SARM1 NNNACAACAGGAAAATCCTA NGG 2 0.1548 Tier II
15 NC_000003.12 3 50103207 - RBM5 NNNAAAACAGCAATATCCTA NAG 2 0.1481 Tier II
16 NC_000005.10 5 52915292 + ITGA1 NNNAAAACAGCAATATCCTC NGG 2 0.1299 Tier II
17 NC_000016.10 16 70952047 - HYDIN NNNATAACAGCAATTTCCTA NGG 2 0.1273 Tier II
18 NC_000008.11 8 23001423 + RHOBTB2 NNNACATCAGCAACATCCTA NGG 2 0.125 Tier II
19 NC_000003.12 3 65482010 - MAGI1 NNNACAACAGCAATTTCATA NGG 2 0.1077 Tier II
20 NC_000003.12 3 27178396 + NEK10 NNNACAACAGCTATATCCTA NAG 2 0.0864 Tier II
21 NC_000014.9 14 79696531 - NRXN3 NNNAGAACAGCAATATGCTA NGG 2 0.0353 Tier II
22 NC_000001.11 1 185194528 - SWT1 NNNACAACAGCAATATCCTT NTG 2 0.0234 Tier II
23 NC_000021.9 21 26488800 + CYYR1-AS1 NNNACAAGAGCCATATCCTA NGG 2 0.1692 Tier III
24 NC_000007.14 7 90167007 + STEAP2-AS1 NNNACAACTGCAATATCCCA NGG 2 0.1655 Tier III
25 NC_000005.10 5 169519164 - LOC105377714 NNNACAACAGCAATCTACTA NGG 2 0.1273 Tier III
26 NC_000005.10 5 169519164 - LOC105377715 NNNACAACAGCAATCTACTA NGG 2 0.1273 Tier III
27 NC_000001.11 1 146765418 + HYDIN2 NNNATAACAGCAATTTCCTA NGG 2 0.1273 Tier III
28 NC_000008.11 8 129423055 + CCDC26 NNNCCAACAGCAATATCCCA NGG 2 0.0974 Tier III
29 NC_000013.11 13 100818822 + NALCN-AS1 NNNACAACAGCAATATGATA NGG 2 0.0317 Tier III
30 NC_000013.11 13 48188212 + LOC105370198 NNNAAAACAGCAAGATCCTA NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 69742910 + Polr2a NNNACAACAGCAATGACCTA NGG 2 0.5909 Tier I
2 NC_000068.7 2 69908931 - Ubr3 NNNACAACAGAAATATCCTT NGG 2 0.45 Tier II
3 NC_000070.6 4 112741495 + Skint10 NNNACAAAAGCAATACCCTA NGG 2 0.4333 Tier II
4 NC_000080.6 14 87005296 - Diaph3 NNNACAACTGCAATATTCTA NGG 2 0.28 Tier II
5 NC_000071.6 5 17656909 + Sema3c NNNACAACAACAATATCCCA NGG 2 0.2575 Tier II
6 NC_000070.6 4 102405602 - Pde4b NNNACAACAGCAATAACCTA NAG 2 0.2357 Tier II
7 NC_000069.6 3 152276027 + Miga1 NNNACAACAGTAATATCCTG NGG 2 0.2353 Tier II
8 NC_000076.6 10 83602102 + Appl2 NNNACAACAGAAATATCCCA NGG 2 0.2069 Tier II
9 NC_000077.6 11 59793706 + Flcn NNNACAACAGCAAACTCCTA NGG 2 0.1688 Tier II
10 NC_000070.6 4 77787289 + Ptprd NNNGCAACAGCAATATCCTA NAG 2 0.162 Tier II
11 NC_000067.6 1 167697947 - Lmx1a NNNACAACAGCACTGTCCTA NGG 2 0.1368 Tier II
12 NC_000075.6 9 28579577 + Opcml NNNACATCAGCAATATCCTC NGG 2 0.0994 Tier II
13 NC_000079.6 13 108801469 + Pde4d NNNACAACAGCAATAGCCTA NAG 2 0.0897 Tier II
14 NC_000068.7 2 164075532 - Stk4 NNNACACCAGCAATTTCCTA NGG 2 0.0875 Tier II
15 NC_000083.6 17 64688434 - Man2a1 NNNACAACAGCAATTTCCTA NAG 2 0.0519 Tier II
16 NC_000074.6 8 16942980 - Csmd1 NNNACAACAGCAATGTCCTA NTG 2 0.0253 Tier II
17 NC_000072.6 6 33265238 - Exoc4 NNNACAACAGCAATAACCTA NGC 2 0.0202 Tier II
18 NC_000068.7 2 66533562 - Scn9a NNNACAACAGCAATATCCAA NGA 2 0.0198 Tier II
19 NC_000067.6 1 84769973 + Trip12 NNNACAATAGCAATATCCTA NGT 2 0.0141 Tier II
20 NC_000070.6 4 86288568 + Adamtsl1 NNNACAACAGCAATAGCCTA NGT 2 0.0056 Tier II
21 NC_000071.6 5 45772983 - Lcorl NNNACAACAGCAATAGCCTA NGT 2 0.0056 Tier II
22 NC_000080.6 14 22742637 + Lrmda NNNACAACAGCAATATCCTA NAA 2 0.0 Tier II
23 NC_000068.7 2 136473679 - Gm32546 NNNACAACAACAAAATCCTA NGG 2 0.5778 Tier III
24 NC_000084.6 18 6290255 + Gm41664 NNNACAACAGCAAAATCCTT NGG 2 0.3714 Tier III
25 NC_000074.6 8 115868540 + Gm31037 NNNACAATAGCAATATCCTC NGG 2 0.1989 Tier III
26 NC_000077.6 11 25673594 + 5730522E02Rik NNNGCAACAGCAACATCCTA NGG 2 0.1786 Tier III
27 NC_000067.6 1 109833236 - Gm8141 NNNACAACAGCATCATCCTA NGG 2 0.0857 Tier III
28 NC_000068.7 2 66533562 - Gm13629 NNNACAACAGCAATATCCAA NGA 2 0.0198 Tier III
29 NC_000080.6 14 22742637 + Gm30926 NNNACAACAGCAATATCCTA NAA 2 0.0 Tier III
Download CSV

Other clones with same target sequence:

(none)