Construct: sgRNA BRDN0001149360
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTGTCTGTTCTAGCACTCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGFR2 (2263)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76184
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
121518827 |
- |
FGFR2 |
NNNGTCTGTTCTAGCACTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
31763544 |
+ |
PDE1C |
NNNATCTGTTCTAGCACTCA |
NGG |
2 |
0.8438 |
Tier II |
| 3 |
NC_000010.11 |
10 |
11328016 |
- |
CELF2 |
NNNGTCTGTTCCAGCACTGG |
NGG |
2 |
0.1184 |
Tier II |
| 4 |
NC_000014.9 |
14 |
64066294 |
- |
SYNE2 |
NNNGTCTGTTCTAGAACTCC |
NGG |
2 |
0.0952 |
Tier II |
| 5 |
NC_000010.11 |
10 |
11328016 |
- |
CELF2-AS1 |
NNNGTCTGTTCCAGCACTGG |
NGG |
2 |
0.1184 |
Tier III |
| 6 |
NC_000017.11 |
17 |
30579127 |
+ |
SMURF2P1-LRRC37BP1 |
NNNGTCTGTTCTTGCACTGG |
NGG |
2 |
0.0375 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
130199903 |
- |
Fgfr2 |
NNNGTCTGTTCTAGCACTCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000082.6 |
16 |
60086078 |
- |
Epha6 |
NNNGTCTGTCCTAGCACTCT |
NGG |
2 |
0.3733 |
Tier II |
| 3 |
NC_000086.7 |
X |
42226334 |
+ |
Stag2 |
NNNTTCAGTTCTAGCACTCG |
NGG |
2 |
0.3182 |
Tier II |
| 4 |
NC_000072.6 |
6 |
90710393 |
- |
Iqsec1 |
NNNGTCTGTTCTGGCACTTG |
NGG |
2 |
0.301 |
Tier II |
| 5 |
NC_000072.6 |
6 |
91979332 |
+ |
Fgd5 |
NNNGTCTGTTCTGGCACTCC |
NGG |
2 |
0.2795 |
Tier II |
| 6 |
NC_000071.6 |
5 |
140295144 |
- |
Mad1l1 |
NNNGTCTGTTCTAGGACTCA |
NGG |
2 |
0.0469 |
Tier II |
| 7 |
NC_000084.6 |
18 |
59268647 |
+ |
Chsy3 |
NNNGTCTTTTCTAGCACTCG |
NGC |
2 |
0.0139 |
Tier II |
| 8 |
NC_000071.6 |
5 |
118720538 |
- |
Med13l |
NNNGTCTGTTCTAGCACTTG |
NGT |
2 |
0.0074 |
Tier II |
| 9 |
NC_000073.6 |
7 |
70273350 |
- |
Gm29683 |
NNNGTCTGTTCTAGAACTCC |
NGG |
2 |
0.0952 |
Tier III |
Other clones with same target sequence:
(none)
