Construct: sgRNA BRDN0001149364
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGCACCCGACAGGACGTAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PTK6 (5753)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76490
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
63534950 |
+ |
PTK6 |
NNNACCCGACAGGACGTAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
16928674 |
- |
TBC1D27P |
NNNACCCACCAGGACGTAGT |
NGG |
2 |
0.5714 |
Tier III |
| 3 |
NC_000016.10 |
16 |
17471095 |
+ |
LOC105371106 |
NNNACCCCTCAGGACGTAGT |
NGG |
2 |
0.3692 |
Tier III |
| 4 |
NC_000003.12 |
3 |
120494889 |
+ |
LOC107986121 |
NNNACCTGACAGGATGTAGT |
NGG |
2 |
0.0542 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
3711830 |
+ |
Osbp2 |
NNNACCTGACAGCACGTAGT |
NGG |
2 |
0.3421 |
Tier I |
| 2 |
NC_000078.6 |
12 |
83593471 |
+ |
Zfyve1 |
NNNACCCTACAGGACGTTGT |
NGG |
2 |
0.3125 |
Tier II |
| 3 |
NC_000079.6 |
13 |
73804961 |
+ |
Slc12a7 |
NNNACCCAACAGGACCTAGT |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000067.6 |
1 |
134093807 |
+ |
Gm32039 |
NNNACCCGCAAGGACGTAGT |
NGG |
2 |
0.4952 |
Tier III |
Other clones with same target sequence:
(none)
