Construct: sgRNA BRDN0001149366

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAGTCTGTTGGAAACCAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RIOK2 (55781)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76958

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 97177755 - RIOK2 NNNTCTGTTGGAAACCAGAT NGG 0 1.0 Tier I
2 NC_000018.10 18 13056042 + CEP192 NNNTCTGTTGGAAACCAGTG NGG 2 0.095 Tier I
3 NC_000001.11 1 19449047 - CAPZB NNNTCAGATGGAAACCAGAT NGG 2 0.6933 Tier II
4 NC_000009.12 9 133423523 + ADAMTS13 NNNTCTGTGGGAAACAAGAT NGG 2 0.619 Tier II
5 NC_000004.12 4 162098039 - FSTL5 NNNTATGTTGGAAACCAGAT NGG 1 0.5714 Tier II
6 NC_000007.14 7 84416729 + SEMA3A NNNTTAGTTGGAAACCAGAT NGG 2 0.5515 Tier II
7 NC_000010.11 10 119822751 + INPP5F NNNTCTGTTAGAAACCAGAA NGG 2 0.525 Tier II
8 NC_000001.11 1 65231133 - AK4 NNNTCATTTGGAAACCAGAT NGG 2 0.4952 Tier II
9 NC_000007.14 7 14486368 + DGKB NNNTCTGTGGGAAACCACAT NGG 2 0.2948 Tier II
10 NC_000007.14 7 22430865 - STEAP1B NNNTCTGTTGTAGACCAGAT NGG 2 0.2508 Tier II
11 NC_000005.10 5 162071755 - GABRG2 NNNTCTGGTGGATACCAGAT NGG 2 0.22 Tier II
12 NC_000016.10 16 88405375 + ZNF469 NNNTCTGTTGGAAACCAGAG NGG 1 0.1765 Tier II
13 NC_000020.11 20 43040381 - PTPRT NNNTCTGTTGGCAACCATAT NGG 2 0.1754 Tier II
14 NC_000001.11 1 50093491 + ELAVL4 NNNTCTGTTGGAAACCATAG NGG 2 0.1176 Tier II
15 NC_000013.11 13 108766207 + MYO16 NNNTCTGTTGGAGACCAGAG NGG 2 0.1151 Tier II
16 NC_000002.12 2 232766926 + KCNJ13 NNNTCTGTTTGAAACCTGAT NGG 2 0.1083 Tier II
17 NC_000002.12 2 232766926 + GIGYF2 NNNTCTGTTTGAAACCTGAT NGG 2 0.1083 Tier II
18 NC_000010.11 10 101842647 + KCNIP2 NNNTCTCTTGGAAACCAGAC NGG 2 0.0625 Tier II
19 NC_000003.12 3 116198178 + LSAMP NNNTCTGTAGGAAATCAGAT NGG 2 0.0619 Tier II
20 NC_000021.9 21 44290912 + AIRE NNNTCTGTTGGAGACCAGAT NGA 2 0.0453 Tier II
21 NC_000009.12 9 19522382 - SLC24A2 NNNCCTGTTGGAAACCAGAT NGA 2 0.0449 Tier II
22 NC_000013.11 13 39755992 - COG6 NNNTCTGTTGGAAATCAGAT NAG 2 0.0173 Tier II
23 NC_000015.10 15 88139372 + NTRK3 NNNTCTGTTGGAAACCAGAG NGA 2 0.0123 Tier II
24 NC_000001.11 1 65428824 + LEPR NNNACTGTTGGAAACCAGAT NGC 2 0.0106 Tier II
25 NC_000001.11 1 65428824 + LEPROT NNNACTGTTGGAAACCAGAT NGC 2 0.0106 Tier II
26 NC_000012.12 12 28188957 - LOC729291 NNNTCTGCTGGAAACCAGAT NAG 2 0.2593 Tier III
27 NC_000006.12 6 108156789 - OSTM1-AS1 NNNTCTGCTGGAAACCGGAT NGG 2 0.1765 Tier III
28 NC_000004.12 4 131535129 + LINC02377 NNNTCTGTTTGAAACCAGAG NGG 2 0.1434 Tier III
29 NC_000012.12 12 129966612 + LOC105370076 NNNTCTGCTGGAAACCAGAC NGG 2 0.0909 Tier III
30 NC_000002.12 2 219353361 + LOC107985835 NNNTCTGGTGGAAATCAGAT NGG 2 0.0489 Tier III
31 NC_000009.12 9 19522382 - LOC105375988 NNNCCTGTTGGAAACCAGAT NGA 2 0.0449 Tier III
32 NC_000001.11 1 72675551 + LOC105378797 NNNTATGTTGGAAAGCAGAT NGG 2 0.0286 Tier III
33 NC_000013.11 13 90531464 + LINC01049 NNNTCTGTTGGAAAACAGAT NTG 2 0.0087 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 17378577 + Riok2 NNNTCTGTTGGAAACCAGAT NGG 0 1.0 Tier I
2 NC_000077.6 11 34239719 + Dock2 NNNTCTGTTGAAAACAAGAT NGG 2 1.0 Tier II
3 NC_000074.6 8 95289853 - Cngb1 NNNTCTGTCAGAAACCAGAT NGG 2 0.8615 Tier II
4 NC_000071.6 5 103157905 + Mapk10 NNNTCTGTTGAGAACCAGAT NGG 2 0.7222 Tier II
5 NC_000073.6 7 72117421 - Mctp2 NNNTATGTTAGAAACCAGAT NGG 2 0.5333 Tier II
6 NC_000074.6 8 16426771 + Csmd1 NNNGCTGTTTGAAACCAGAT NGG 2 0.5078 Tier II
7 NC_000071.6 5 130982986 + Galnt17 NNNTCTGTTGGAAACAACAT NGG 2 0.4762 Tier II
8 NC_000077.6 11 114734379 + Dnaic2 NNNGCTGTTGGGAACCAGAT NGG 2 0.4514 Tier II
9 NC_000078.6 12 33059623 + Cdhr3 NNNTCTGTAGGAAACCAGGT NGG 2 0.3482 Tier II
10 NC_000080.6 14 11904865 + Ptprg NNNTATGTTGCAAACCAGAT NGG 2 0.2449 Tier II
11 NC_000072.6 6 46566318 - Cntnap2 NNNTCTGTTGCAAACCAGAA NGG 2 0.2411 Tier II
12 NC_000073.6 7 28579723 + Pak4 NNNTCTGTTGAAAACCAGCT NGG 2 0.2069 Tier II
13 NC_000073.6 7 4701934 - Brsk1 NNNTCTGTTAGAAACCAGCT NGG 2 0.1931 Tier II
14 NC_000068.7 2 180210243 - Lama5 NNNTCTGTTAGAAACCTGAT NGG 2 0.1244 Tier II
15 NC_000081.6 15 65863235 + Efr3a NNNTCTGATGGAAACCTGAT NGG 2 0.1067 Tier II
16 NC_000074.6 8 123953128 + Abcb10 NNNTCTTTTGGAAACCAGAG NGG 2 0.1008 Tier II
17 NC_000067.6 1 15302457 - Kcnb2 NNNTCTGTTGTAAAACAGAT NGG 2 0.0855 Tier II
18 NC_000074.6 8 124863903 - Gnpat NNNTCTGTTGGAAACCAAAC NGG 2 0.0629 Tier II
19 NC_000071.6 5 101879185 - Wdfy3 NNNTCTGTTAGAAATCAGAT NGG 2 0.0622 Tier II
20 NC_000078.6 12 111891541 - Ppp1r13b NNNTGTGTTGGAAACCAGAC NGG 2 0.0545 Tier II
21 NC_000072.6 6 63778868 - Grid2 NNNTCTGTTGGAAAAGAGAT NGG 2 0.0342 Tier II
22 NC_000085.6 19 17741741 - Pcsk5 NNNCCTGTTGGAAAGCAGAT NGG 2 0.0324 Tier II
23 NC_000071.6 5 36333274 + Sorcs2 NNNTCTGTTGGAAGCCAGAT NGA 2 0.0324 Tier II
24 NC_000068.7 2 140365376 + Sel1l2 NNNTCTGTTGGAAGGCAGAT NGG 2 0.0233 Tier II
25 NC_000078.6 12 28576997 + Allc NNNTCTGTGGGAAACCAGAT NGC 2 0.0138 Tier II
26 NC_000075.6 9 50974361 - Sik2 NNNTCTTTTGGAAACCAGAT NGT 2 0.0092 Tier II
27 NC_000080.6 14 116210684 - Gpc5 NNNTCTGTTGGAAATCAGAT NGT 2 0.0011 Tier II
28 NC_000083.6 17 9384141 + Gm7947 NNNTCAGTTTGAAACCAGAT NGG 2 0.7042 Tier III
29 NC_000073.6 7 72117421 - Gm36696 NNNTATGTTAGAAACCAGAT NGG 2 0.5333 Tier III
30 NC_000082.6 16 97668125 - Gm41512 NNNTCTGTTAGAAAACAGAT NGG 2 0.2074 Tier III
31 NC_000079.6 13 47439268 - Gm35733 NNNGCTGTTGGAAATCAGAT NGG 2 0.0417 Tier III
32 NC_000075.6 9 115524855 + Gm39447 NNNTCTGTTAGAAACCAGAT NTG 2 0.0364 Tier III
33 NC_000068.7 2 163139255 + Gm11454 NNNTCTGTTGGGAAGCAGAT NGG 2 0.0361 Tier III
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Other clones with same target sequence:

(none)