Construct: sgRNA BRDN0001149367
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTGCGGAAGCACCCCGGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ADCK1 (57143)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76087
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
77924560 |
+ |
ADCK1 |
NNNGCGGAAGCACCCCGGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
3729276 |
+ |
DLGAP1 |
NNNGCGGAAGCAGCCGGGCA |
NGG |
2 |
0.021 |
Tier I |
| 3 |
NC_000010.11 |
10 |
132545523 |
- |
INPP5A |
NNNGCGGGAACACCCCGGCA |
NGG |
2 |
0.6844 |
Tier II |
| 4 |
NC_000007.14 |
7 |
92293192 |
- |
ANKIB1 |
NNNGGGGAAGCACCCAGGCA |
NGG |
2 |
0.6 |
Tier II |
| 5 |
NC_000019.10 |
19 |
19498743 |
- |
GATAD2A |
NNNGCGGAAGCAGCCCGGCT |
NGG |
2 |
0.0818 |
Tier II |
| 6 |
NC_000020.11 |
20 |
61900666 |
- |
CDH4 |
NNNGCGGAAGCACCGCGGCT |
NGG |
2 |
0.03 |
Tier II |
| 7 |
NC_000001.11 |
1 |
116168419 |
+ |
LOC124904384 |
NNNGCGGGAGCACCCCGCCA |
NGG |
2 |
0.3492 |
Tier III |
| 8 |
NC_000002.12 |
2 |
66581492 |
- |
LINC01798 |
NNNGCGGAGCCACCCCGGCA |
NGG |
2 |
0.2667 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
70662669 |
- |
Dlgap1 |
NNNGCGGAAGCAGCCAGGCA |
NGG |
2 |
0.1364 |
Tier I |
| 2 |
NC_000071.6 |
5 |
30073486 |
- |
Tyms |
NNNGCGGAAGCCCCACGGCA |
NGG |
2 |
0.0585 |
Tier I |
Other clones with same target sequence:
(none)
