Construct: sgRNA BRDN0001149371
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTCCAGCACCGCTCCACCG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- CAMK2A (815)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000005.10 | 5 | 150250300 | - | CAMK2A | NNNCCAGCACCGCTCCACCG | NGG | 0 | 1.0 | Tier I |
2 | NC_000002.12 | 2 | 233719291 | - | UGT1A4 | NNNCCAGCACCGCCCCACAG | NGG | 2 | 0.1224 | Tier I |
3 | NC_000007.14 | 7 | 291336 | + | FOXL3 | NNNCCAGCCCCGCTCCCCCG | NGG | 2 | 0.1008 | Tier I |
4 | NC_000009.12 | 9 | 136846893 | + | AJM1 | NNNCCGGCACCGCGCCACCG | NGG | 2 | 0.0 | Tier I |
5 | NC_000023.11 | X | 153334252 | + | ZNF275 | NNNCCAGCGCCGCTCCACCG | NGG | 1 | 0.6667 | Tier II |
6 | NC_000015.10 | 15 | 51751656 | + | TMOD2 | NNNCCGGCCCCGCTCCACCG | NGG | 2 | 0.4082 | Tier II |
7 | NC_000004.12 | 4 | 7216324 | + | SORCS2 | NNNCCAGCACTGCTCCACCT | NGG | 2 | 0.2154 | Tier II |
8 | NC_000004.12 | 4 | 115015082 | + | NDST4 | NNNCCAGCAGCGCTCCACTG | NGG | 2 | 0.1795 | Tier II |
9 | NC_000002.12 | 2 | 233719291 | - | UGT1A10 | NNNCCAGCACCGCCCCACAG | NGG | 2 | 0.1224 | Tier II |
10 | NC_000002.12 | 2 | 233719291 | - | UGT1A8 | NNNCCAGCACCGCCCCACAG | NGG | 2 | 0.1224 | Tier II |
11 | NC_000002.12 | 2 | 233719291 | - | UGT1A7 | NNNCCAGCACCGCCCCACAG | NGG | 2 | 0.1224 | Tier II |
12 | NC_000002.12 | 2 | 233719291 | - | UGT1A6 | NNNCCAGCACCGCCCCACAG | NGG | 2 | 0.1224 | Tier II |
13 | NC_000002.12 | 2 | 233719291 | - | UGT1A5 | NNNCCAGCACCGCCCCACAG | NGG | 2 | 0.1224 | Tier II |
14 | NC_000002.12 | 2 | 233719291 | - | UGT1A9 | NNNCCAGCACCGCCCCACAG | NGG | 2 | 0.1224 | Tier II |
15 | NC_000009.12 | 9 | 114611320 | + | TMEM268 | NNNCCAGCTCCGCTCCGCCG | NGG | 2 | 0.1059 | Tier II |
16 | NC_000003.12 | 3 | 127983325 | - | KBTBD12 | NNNCCAGCACCGCTACACTG | NGG | 2 | 0.1026 | Tier II |
17 | NC_000021.9 | 21 | 31731752 | - | SCAF4 | NNNCCAGCCCCGCTCCGCCG | NGG | 2 | 0.1008 | Tier II |
18 | NC_000006.12 | 6 | 53065728 | + | FBXO9 | NNNCCAGCACCCCTCCTCCG | NGG | 2 | 0.0706 | Tier II |
19 | NC_000001.11 | 1 | 2499024 | - | PLCH2 | NNNCCAGCACCGCTCCAGCC | NGG | 2 | 0.0571 | Tier II |
20 | NC_000021.9 | 21 | 42442128 | - | UBASH3A | NNNCCAGCACAGCTCCACCG | NGA | 2 | 0.0521 | Tier II |
21 | NC_000002.12 | 2 | 220625662 | - | LOC105373895 | NNNCCAGCACCACTCCACAG | NGG | 2 | 0.4 | Tier III |
22 | NC_000021.9 | 21 | 45236740 | + | LINC00334 | NNNCCAGCACCTCTCCACCC | NGG | 2 | 0.1648 | Tier III |
23 | NC_000002.12 | 2 | 233719291 | - | UGT1A | NNNCCAGCACCGCCCCACAG | NGG | 2 | 0.1224 | Tier III |
24 | NC_000002.12 | 2 | 240385073 | - | LOC102723825 | NNNCCAGCAGCGCCCCACCG | NGG | 2 | 0.1111 | Tier III |
25 | NC_000007.14 | 7 | 291336 | + | FOXL3-OT1 | NNNCCAGCCCCGCTCCCCCG | NGG | 2 | 0.1008 | Tier III |
26 | NC_000002.12 | 2 | 2724175 | + | LOC105373390 | NNNCCAGCACCGCTGCACTG | NGG | 2 | 0.0231 | Tier III |
27 | NC_000022.11 | 22 | 49602757 | + | MIR3667HG | NNNCCAGCACCGCTGCAGCG | NGG | 2 | 0.0067 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000084.6 | 18 | 60958583 | + | Camk2a | NNNCCAGCACCGCTCCACCG | NGG | 0 | 1.0 | Tier I |
2 | NC_000075.6 | 9 | 121590966 | - | Lyzl4 | NNNCCAGCACAGCTCCACCC | NGG | 2 | 0.3214 | Tier II |
3 | NC_000073.6 | 7 | 37624146 | - | Zfp536 | NNNACAGCACCGCTGCACCG | NGG | 2 | 0.0421 | Tier II |
4 | NC_000076.6 | 10 | 90499710 | - | Anks1b | NNNCCAGCACCGCTCCACAG | NGT | 2 | 0.0069 | Tier II |
5 | NC_000075.6 | 9 | 121590966 | - | Lyzl4os | NNNCCAGCACAGCTCCACCC | NGG | 2 | 0.3214 | Tier III |