Construct: sgRNA BRDN0001149371

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCTCCAGCACCGCTCCACCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK2A (815)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75753

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 150250300 - CAMK2A NNNCCAGCACCGCTCCACCG NGG 0 1.0 Tier I
2 NC_000002.12 2 233719291 - UGT1A4 NNNCCAGCACCGCCCCACAG NGG 2 0.1224 Tier I
3 NC_000007.14 7 291336 + FOXL3 NNNCCAGCCCCGCTCCCCCG NGG 2 0.1008 Tier I
4 NC_000009.12 9 136846893 + AJM1 NNNCCGGCACCGCGCCACCG NGG 2 0.0 Tier I
5 NC_000023.11 X 153334252 + ZNF275 NNNCCAGCGCCGCTCCACCG NGG 1 0.6667 Tier II
6 NC_000015.10 15 51751656 + TMOD2 NNNCCGGCCCCGCTCCACCG NGG 2 0.4082 Tier II
7 NC_000004.12 4 7216324 + SORCS2 NNNCCAGCACTGCTCCACCT NGG 2 0.2154 Tier II
8 NC_000004.12 4 115015082 + NDST4 NNNCCAGCAGCGCTCCACTG NGG 2 0.1795 Tier II
9 NC_000002.12 2 233719291 - UGT1A10 NNNCCAGCACCGCCCCACAG NGG 2 0.1224 Tier II
10 NC_000002.12 2 233719291 - UGT1A8 NNNCCAGCACCGCCCCACAG NGG 2 0.1224 Tier II
11 NC_000002.12 2 233719291 - UGT1A7 NNNCCAGCACCGCCCCACAG NGG 2 0.1224 Tier II
12 NC_000002.12 2 233719291 - UGT1A6 NNNCCAGCACCGCCCCACAG NGG 2 0.1224 Tier II
13 NC_000002.12 2 233719291 - UGT1A5 NNNCCAGCACCGCCCCACAG NGG 2 0.1224 Tier II
14 NC_000002.12 2 233719291 - UGT1A9 NNNCCAGCACCGCCCCACAG NGG 2 0.1224 Tier II
15 NC_000009.12 9 114611320 + TMEM268 NNNCCAGCTCCGCTCCGCCG NGG 2 0.1059 Tier II
16 NC_000003.12 3 127983325 - KBTBD12 NNNCCAGCACCGCTACACTG NGG 2 0.1026 Tier II
17 NC_000021.9 21 31731752 - SCAF4 NNNCCAGCCCCGCTCCGCCG NGG 2 0.1008 Tier II
18 NC_000006.12 6 53065728 + FBXO9 NNNCCAGCACCCCTCCTCCG NGG 2 0.0706 Tier II
19 NC_000001.11 1 2499024 - PLCH2 NNNCCAGCACCGCTCCAGCC NGG 2 0.0571 Tier II
20 NC_000021.9 21 42442128 - UBASH3A NNNCCAGCACAGCTCCACCG NGA 2 0.0521 Tier II
21 NC_000002.12 2 220625662 - LOC105373895 NNNCCAGCACCACTCCACAG NGG 2 0.4 Tier III
22 NC_000021.9 21 45236740 + LINC00334 NNNCCAGCACCTCTCCACCC NGG 2 0.1648 Tier III
23 NC_000002.12 2 233719291 - UGT1A NNNCCAGCACCGCCCCACAG NGG 2 0.1224 Tier III
24 NC_000002.12 2 240385073 - LOC102723825 NNNCCAGCAGCGCCCCACCG NGG 2 0.1111 Tier III
25 NC_000007.14 7 291336 + FOXL3-OT1 NNNCCAGCCCCGCTCCCCCG NGG 2 0.1008 Tier III
26 NC_000002.12 2 2724175 + LOC105373390 NNNCCAGCACCGCTGCACTG NGG 2 0.0231 Tier III
27 NC_000022.11 22 49602757 + MIR3667HG NNNCCAGCACCGCTGCAGCG NGG 2 0.0067 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 60958583 + Camk2a NNNCCAGCACCGCTCCACCG NGG 0 1.0 Tier I
2 NC_000075.6 9 121590966 - Lyzl4 NNNCCAGCACAGCTCCACCC NGG 2 0.3214 Tier II
3 NC_000073.6 7 37624146 - Zfp536 NNNACAGCACCGCTGCACCG NGG 2 0.0421 Tier II
4 NC_000076.6 10 90499710 - Anks1b NNNCCAGCACCGCTCCACAG NGT 2 0.0069 Tier II
5 NC_000075.6 9 121590966 - Lyzl4os NNNCCAGCACAGCTCCACCC NGG 2 0.3214 Tier III
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Other clones with same target sequence:

(none)