Construct: sgRNA BRDN0001149372
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAAGCTCCAATAATCAACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CHUK (1147)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77033
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
100219093 |
+ |
CHUK |
NNNGCTCCAATAATCAACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
100853041 |
+ |
ST8SIA4 |
NNNACTCCAGTAATCAACAG |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000005.10 |
5 |
59385218 |
- |
PDE4D |
NNNGATCCAATAATCAACAC |
NGG |
2 |
0.2449 |
Tier II |
| 4 |
NC_000023.11 |
X |
14747653 |
+ |
FANCB |
NNNGCTCCAAGTATCAACAG |
NGG |
2 |
0.1333 |
Tier II |
| 5 |
NC_000001.11 |
1 |
39900721 |
+ |
MYCL |
NNNGCTCCAATAATCATCAA |
NGG |
2 |
0.125 |
Tier II |
| 6 |
NC_000015.10 |
15 |
101643211 |
+ |
TM2D3 |
NNNGCTCCAATAATCAACAC |
NTG |
2 |
0.0167 |
Tier II |
| 7 |
NC_000011.10 |
11 |
114361706 |
- |
LOC101928940 |
NNNGCTCCATAAATCAACAG |
NGG |
2 |
0.6618 |
Tier III |
| 8 |
NC_000006.12 |
6 |
119580610 |
+ |
LOC105377975 |
NNNGCTCCAATAATCAAAAC |
NGG |
2 |
0.2308 |
Tier III |
| 9 |
NC_000009.12 |
9 |
33677551 |
- |
PTENP1-AS |
NNNGCTGCAATAATCAACAG |
NGT |
2 |
0.0076 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
44097288 |
+ |
Chuk |
NNNGCTCCAATAATCCACAG |
NGG |
1 |
0.0 |
Tier I |
| 2 |
NC_000080.6 |
14 |
64397629 |
+ |
Msra |
NNNACACCAATAATCAACAG |
NGG |
2 |
0.78 |
Tier II |
| 3 |
NC_000076.6 |
10 |
86822027 |
- |
Nt5dc3 |
NNNGCTTCAATAAACAACAG |
NGG |
2 |
0.503 |
Tier II |
| 4 |
NC_000079.6 |
13 |
95996737 |
+ |
Sv2c |
NNNGCACCAATATTCAACAG |
NGG |
2 |
0.26 |
Tier II |
| 5 |
NC_000081.6 |
15 |
78461962 |
+ |
Tmprss6 |
NNNGCTCCAAGATTCAACAG |
NGG |
2 |
0.12 |
Tier II |
| 6 |
NC_000083.6 |
17 |
44969472 |
- |
Supt3 |
NNNCCTCCAATAATCACCAG |
NGG |
2 |
0.0934 |
Tier II |
| 7 |
NC_000081.6 |
15 |
97347953 |
- |
Pced1b |
NNNGCTCCAAGAATCAGCAG |
NGG |
2 |
0.0706 |
Tier II |
| 8 |
NC_000082.6 |
16 |
49872100 |
- |
Cd47 |
NNNGCTCCAAGAATCAACAG |
NTG |
2 |
0.0156 |
Tier II |
| 9 |
NC_000082.6 |
16 |
55883918 |
+ |
Nxpe3 |
NNNGTTCCAATAATCCACAG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000067.6 |
1 |
45889145 |
- |
Gm5269 |
NNNGCTACAATAAACAACAG |
NGG |
2 |
0.4643 |
Tier III |
| 11 |
NC_000069.6 |
3 |
38204191 |
+ |
5430434I15Rik |
NNNGCTCCCATAGTCAACAG |
NGG |
2 |
0.3727 |
Tier III |
| 12 |
NC_000081.6 |
15 |
97347953 |
- |
Gm33325 |
NNNGCTCCAAGAATCAGCAG |
NGG |
2 |
0.0706 |
Tier III |
| 13 |
NC_000082.6 |
16 |
55883918 |
+ |
Gm17809 |
NNNGTTCCAATAATCCACAG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
