Construct: sgRNA BRDN0001149376

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTAACCAAAGCTGATTGACT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLT3 (2322)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77349

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000013.11 13 28049409 + FLT3 NNNACCAAAGCTGATTGACT NGG 0 1.0 Tier I
2 NC_000016.10 16 17358957 + XYLT1 NNNACAAAAGCCGATTGACT NGG 2 0.8797 Tier II
3 NC_000017.11 17 11653161 + DNAH9 NNNACTAAAGCTGATAGACT NGG 2 0.8442 Tier II
4 NC_000012.12 12 59620314 + SLC16A7 NNNAGCAAAACTGATTGACT NGG 2 0.56 Tier II
5 NC_000002.12 2 124089133 - CNTNAP5 NNNAACAAAGCTGATTGTCT NGG 2 0.2857 Tier II
6 NC_000003.12 3 17177096 + TBC1D5 NNNACCAAAGATGATGGACT NGG 2 0.2596 Tier II
7 NC_000004.12 4 94253235 + SMARCAD1 NNNACCCAAGCTGATTGGCT NGG 2 0.175 Tier II
8 NC_000001.11 1 3466312 + ARHGEF16 NNNACCAAAGCTGCTTGACA NGG 2 0.1205 Tier II
9 NC_000002.12 2 102240779 - IL1RL2 NNNAACAAAGCTGATTGACC NGG 2 0.0519 Tier II
10 NC_000001.11 1 109162047 - ELAPOR1 NNNACCAAAGCTGAGTGACA NGG 2 0.0281 Tier II
11 NC_000011.10 11 113021540 - NCAM1 NNNACCAAAGCTGATTGTCT NTG 2 0.0195 Tier II
12 NC_000023.11 X 44236280 + EFHC2 NNNACCAAAGCTGATTGCCT NGT 2 0.0031 Tier II
13 NC_000015.10 15 25572524 - LINC02250 NNNACCAAAACTGATAGACT NGG 2 0.8485 Tier III
14 NC_000006.12 6 28402358 + LOC105374998 NNNACCAAGGATGATTGACT NGG 2 0.5 Tier III
15 NC_000004.12 4 120141919 - MAD2L1-DT NNNACCAAGGCTGTTTGACT NGG 2 0.3556 Tier III
16 NC_000007.14 7 20882030 + LINC01162 NNNAACAAAGCTGATTGGCT NGG 2 0.2286 Tier III
17 NC_000023.11 X 57661688 + LOC107985708 NNNACTAAAGCTGATTCACT NGG 2 0.2185 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 143991533 + Pramel14 NNNACCAAAGCAGACTGACT NGG 2 0.2182 Tier I
2 NC_000073.6 7 99582851 - Arrb1 NNNACCAAATCTAATTGACT NGG 2 0.75 Tier II
3 NC_000071.6 5 100890270 + Gpat3 NNNAGAAAAGCTGATTGACT NGG 2 0.5571 Tier II
4 NC_000082.6 16 13618887 - Parn NNNATCAAAGTTGATTGACT NGG 2 0.1958 Tier II
5 NC_000072.6 6 65075403 + Smarcad1 NNNACCCAAGCTGATTGGCT NGG 2 0.175 Tier II
6 NC_000074.6 8 31202453 + Fut10 NNNACCAAAGCTGATAGCCT NGG 2 0.1732 Tier II
7 NC_000075.6 9 44148741 - Cbl NNNCCCTAAGCTGATTGACT NGG 2 0.1544 Tier II
8 NC_000070.6 4 113179485 - Skint6 NNNACCAAAGGTGATTTACT NGG 2 0.0625 Tier II
9 NC_000077.6 11 12248983 + Cobl NNNATCAAAGCTGAGTGACT NGG 2 0.0318 Tier II
10 NC_000081.6 15 58891231 + Tatdn1 NNNACCAAATCTGATTGACT NTG 2 0.0317 Tier II
11 NC_000081.6 15 58891231 + Rnf139 NNNACCAAATCTGATTGACT NTG 2 0.0317 Tier II
12 NC_000086.7 X 127041945 + Gm382 NNNACCAAAGCTGATTGACA NGT 2 0.0091 Tier II
13 NC_000072.6 6 69478373 + Igk NNNAACAAAGATGATTGACT NGG 2 0.4286 Tier III
14 NC_000072.6 6 69657798 + Igk NNNAACAAAGATGATTGACT NGG 2 0.4286 Tier III
15 NC_000082.6 16 4267845 - Gm36359 NNNACCCCAGCTGATTGACT NGG 2 0.1875 Tier III
16 NC_000070.6 4 114756153 - Gm33655 NNNATCAAAGCTGACTGACT NGG 2 0.1736 Tier III
17 NC_000086.7 X 12980646 + 4930500G05Rik NNNACCAAACCTTATTGACT NGG 2 0.12 Tier III
18 NC_000086.7 X 12980646 + Gm41890 NNNACCAAACCTTATTGACT NGG 2 0.12 Tier III
19 NC_000071.6 5 42082490 - Gm16223 NNNACCAAAGCTGAATGACT NGT 2 0.0093 Tier III
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Other clones with same target sequence:

(none)