Construct: sgRNA BRDN0001149378
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCTTGTAGTACTTCCCAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CIB4 (130106)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75864
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
26629473 |
+ |
CIB4 |
NNNTTGTAGTACTTCCCAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
4803686 |
+ |
RCL1 |
NNNTTGTAGTGCTTCCCAGG |
NGG |
1 |
0.65 |
Tier II |
| 3 |
NC_000015.10 |
15 |
31867523 |
- |
OTUD7A |
NNNTGGTAGTACTTCCCAGA |
NGG |
2 |
0.6 |
Tier II |
| 4 |
NC_000003.12 |
3 |
37302712 |
+ |
GOLGA4 |
NNNTTGTAGAACTTCCCAGT |
NGG |
2 |
0.6 |
Tier II |
| 5 |
NC_000015.10 |
15 |
68387419 |
- |
ITGA11 |
NNNTTCTAGAACTTCCCAGG |
NGG |
2 |
0.5844 |
Tier II |
| 6 |
NC_000012.12 |
12 |
51198269 |
+ |
POU6F1 |
NNNTTGTAGTCCTTCCCAGG |
NGG |
1 |
0.4 |
Tier II |
| 7 |
NC_000023.11 |
X |
6813912 |
+ |
PUDP |
NNNTTGTAGTGCTTCCAAGG |
NGG |
2 |
0.3033 |
Tier II |
| 8 |
NC_000003.12 |
3 |
7246290 |
- |
GRM7 |
NNNTTATAGTACTTCCCAGG |
NAG |
2 |
0.2593 |
Tier II |
| 9 |
NC_000017.11 |
17 |
59369774 |
+ |
YPEL2 |
NNNTTGTAGTAGTTCCCTGG |
NGG |
2 |
0.2222 |
Tier II |
| 10 |
NC_000010.11 |
10 |
77416436 |
- |
KCNMA1 |
NNNTTGTAGTATTTCCCGGG |
NGG |
2 |
0.2154 |
Tier II |
| 11 |
NC_000023.11 |
X |
65674104 |
+ |
MSN |
NNNTTGTAGCACTTCCCAGG |
NAG |
2 |
0.1383 |
Tier II |
| 12 |
NC_000004.12 |
4 |
67487757 |
- |
CENPC |
NNNTTGTAGAACTTCCCAGG |
NGA |
2 |
0.0595 |
Tier II |
| 13 |
NC_000002.12 |
2 |
29850750 |
+ |
ALK |
NNNTTGTAGTACTTCCCAAG |
NGA |
2 |
0.0496 |
Tier II |
| 14 |
NC_000017.11 |
17 |
10106025 |
- |
GAS7 |
NNNTTATAGTACTGCCCAGG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000014.9 |
14 |
48991648 |
- |
LOC105378178 |
NNNTTGTGGTACATCCCAGG |
NGG |
2 |
0.5077 |
Tier III |
| 16 |
NC_000003.12 |
3 |
72066103 |
+ |
LINC00877 |
NNNTTTTAGTACTTCCCATG |
NGG |
2 |
0.4444 |
Tier III |
| 17 |
NC_000008.11 |
8 |
118624102 |
- |
SAMD12-AS1 |
NNNTTTTAGTACTTCCCAGG |
NGA |
2 |
0.0463 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
43418187 |
+ |
Zbtb20 |
NNNTTGCAGAACTTCCCAGG |
NGG |
2 |
0.5893 |
Tier II |
| 2 |
NC_000075.6 |
9 |
109027142 |
- |
Pfkfb4 |
NNNTGGTAGGACTTCCCAGG |
NGG |
2 |
0.32 |
Tier II |
| 3 |
NC_000070.6 |
4 |
65647624 |
+ |
Astn2 |
NNNTGGTAGTACTTCCCTGG |
NGG |
2 |
0.32 |
Tier II |
| 4 |
NC_000070.6 |
4 |
104442493 |
- |
Dab1 |
NNNATGTAGTACTTCCCATG |
NGG |
2 |
0.3175 |
Tier II |
| 5 |
NC_000076.6 |
10 |
62875364 |
- |
Tet1 |
NNNTTCTTGTACTTCCCAGG |
NGG |
2 |
0.2922 |
Tier II |
| 6 |
NC_000069.6 |
3 |
8933750 |
+ |
Tpd52 |
NNNTTGTAGTACTACCCAGC |
NGG |
2 |
0.2653 |
Tier II |
| 7 |
NC_000086.7 |
X |
71967837 |
- |
Prrg3 |
NNNCTGTAGTTCTTCCCAGG |
NGG |
2 |
0.1991 |
Tier II |
| 8 |
NC_000068.7 |
2 |
102305911 |
+ |
Trim44 |
NNNTTCTAGTACTTCCCAGG |
NAG |
2 |
0.1768 |
Tier II |
| 9 |
NC_000083.6 |
17 |
52906824 |
+ |
Kcnh8 |
NNNTTGTAGTTCTTCCCACG |
NGG |
2 |
0.1379 |
Tier II |
| 10 |
NC_000077.6 |
11 |
76216786 |
+ |
Gemin4 |
NNNTTGGAGTACTTCCCAGG |
NCG |
2 |
0.063 |
Tier II |
| 11 |
NC_000070.6 |
4 |
115887318 |
- |
Kncn |
NNNTTGTAGTACTTCCCAGC |
NTG |
2 |
0.0167 |
Tier II |
| 12 |
NC_000082.6 |
16 |
43418187 |
+ |
Gm15713 |
NNNTTGCAGAACTTCCCAGG |
NGG |
2 |
0.5893 |
Tier III |
| 13 |
NC_000070.6 |
4 |
84073770 |
- |
Gm33410 |
NNNCTGTAGTACTTCCCATG |
NGG |
2 |
0.4314 |
Tier III |
| 14 |
NC_000070.6 |
4 |
84073770 |
- |
Gm33480 |
NNNCTGTAGTACTTCCCATG |
NGG |
2 |
0.4314 |
Tier III |
| 15 |
NC_000076.6 |
10 |
62875364 |
- |
Gm51805 |
NNNTTCTTGTACTTCCCAGG |
NGG |
2 |
0.2922 |
Tier III |
Other clones with same target sequence:
(none)
