Construct: sgRNA BRDN0001149381

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGTTCTGAGAGCAAGACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK26 (51765)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76000

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 132068421 - STK26 NNNTTCTGAGAGCAAGACAT NGG 0 1.0 Tier I
2 NC_000010.11 10 49664994 + CHAT NNNTACTGAGAGCAAGCCAT NGG 2 0.0882 Tier I
3 NC_000018.10 18 58536406 - ALPK2 NNNTTCTGAGAGCAAGGCAT NAG 2 0.0458 Tier I
4 NC_000017.11 17 51245733 - MBTD1 NNNTTCAGAAAGCAAGACAT NGG 2 0.8167 Tier II
5 NC_000003.12 3 10676394 + ATP2B2 NNNTTCTATGAGCAAGACAT NGG 2 0.6 Tier II
6 NC_000001.11 1 112725173 + TAFA3 NNNTTCTGAGAGCTAAACAT NGG 2 0.5333 Tier II
7 NC_000003.12 3 131691489 + CPNE4 NNNTTCTGAGAGCTAAACAT NGG 2 0.5333 Tier II
8 NC_000019.10 19 39733522 + CLC NNNTTGTGAGAGCAAAACAT NGG 2 0.5 Tier II
9 NC_000019.10 19 15887026 + CYP4F2 NNNTGCTGTGAGCAAGACAT NGG 2 0.384 Tier II
10 NC_000009.12 9 96513999 + CDC14B NNNTTTTGAGAGAAAGACAT NGG 2 0.3571 Tier II
11 NC_000001.11 1 197306636 + CRB1 NNNTGCTGAGAGCAAGACTT NGG 2 0.3446 Tier II
12 NC_000015.10 15 75364858 - MAN2C1 NNNCTCTGACAGCAAGACAT NGG 2 0.2588 Tier II
13 NC_000001.11 1 67715277 - GNG12 NNNGTCTGAGATCAAGACAT NGG 2 0.2404 Tier II
14 NC_000006.12 6 124193552 - NKAIN2 NNNTTCTGGGAGCCAGACAT NGG 2 0.1429 Tier II
15 NC_000014.9 14 78545261 + NRXN3 NNNTTCTGAGGGCAAGACCT NGG 2 0.1345 Tier II
16 NC_000008.11 8 51547606 - PXDNL NNNTTCTGAGAGCCAGACTT NGG 2 0.1154 Tier II
17 NC_000016.10 16 88246152 + ZNF469 NNNTTCTGAGAGCAAGCCAA NGG 2 0.0993 Tier II
18 NC_000011.10 11 67838686 + LOC112268076 NNNTTCTGAGAGCAAGAAAG NGG 2 0.095 Tier II
19 NC_000014.9 14 20691899 - ANG NNNTTCTGAGAGCAAGACTG NGG 2 0.095 Tier II
20 NC_000014.9 14 20691899 - RNASE4 NNNTTCTGAGAGCAAGACTG NGG 2 0.095 Tier II
21 NC_000015.10 15 59133014 + MYO1E NNNTTCTGAGAGGAAGATAT NGG 2 0.0877 Tier II
22 NC_000002.12 2 135004927 + MAP3K19 NNNTTCTGAGAGGAAGAAAT NGG 2 0.0734 Tier II
23 NC_000004.12 4 88400719 + HERC6 NNNTTCTGAGAGAAAGACAG NGG 2 0.0679 Tier II
24 NC_000023.11 X 2761358 - XG NNNTTCTGAGATCAAGGCAT NGG 2 0.0679 Tier II
25 NC_000005.10 5 55109167 - GZMA NNNTTCTGAAAGCAAGACAT NGA 2 0.0648 Tier II
26 NC_000011.10 11 35409043 - SLC1A2 NNNTTCTGAGACCAAGACAT NGA 2 0.0368 Tier II
27 NC_000002.12 2 36704760 - VIT NNNTTCTGAGAGGCAGACAT NGG 2 0.0292 Tier II
28 NC_000023.11 X 65517745 - LAS1L NNNTTCTGAGAGGATGACAT NGG 2 0.0273 Tier II
29 NC_000012.12 12 117532556 + KSR2 NNNTTCTGAGATCAAGACAT NGA 2 0.0267 Tier II
30 NC_000006.12 6 161671173 - PRKN NNNTTCTGAGAGCAAGACAC NAG 2 0.0236 Tier II
31 NC_000009.12 9 6782529 - KDM4C NNNTTCTGTGAGCAAGACAT NTG 2 0.0234 Tier II
32 NC_000006.12 6 139269091 - TXLNB NNNTTATGAGAGCAAGACAT NGT 2 0.015 Tier II
33 NC_000003.12 3 57368295 + DNAH12 NNNTTCTGAGAGGAAGACAT NGA 2 0.0095 Tier II
34 NC_000005.10 5 5195781 + ADAMTS16 NNNTTCTGAGAGCAAGGCAT NGT 2 0.0028 Tier II
35 NC_000004.12 4 73119298 - ANKRD17 NNNTTCTGAGAGCAAGACAT NTT 2 0.0 Tier II
36 NC_000005.10 5 11054971 + CTNND2 NNNTTCTGAGAGCAATGCAT NGG 2 0.0 Tier II
37 NC_000009.12 9 15447867 - SNAPC3 NNNTTTTGAGAGCAACACAT NGG 2 0.0 Tier II
38 NC_000002.12 2 235271980 + LOC105373940 NNNTTCTAATAGCAAGACAT NGG 2 0.8125 Tier III
39 NC_000008.11 8 114938049 + LOC107986901 NNNTACTGAAAGCAAGACAT NGG 2 0.4667 Tier III
40 NC_000002.12 2 67635204 - LOC105374786 NNNTGCTGAGACCAAGACAT NGG 2 0.3388 Tier III
41 NC_000001.11 1 187168167 + LINC01036 NNNTTCTCAGAGCAAGAAAT NGG 2 0.3314 Tier III
42 NC_000007.14 7 41481868 + LOC107986787 NNNTGCTGAGAGAAAGACAT NGG 2 0.2462 Tier III
43 NC_000008.11 8 39517215 + ADAM3A NNNTCCTGAGAGCAAGGCAT NGG 2 0.1765 Tier III
44 NC_000004.12 4 9111700 - ENPP7P10 NNNTTCTGAGAGCAAGAAAG NGG 2 0.095 Tier III
45 NC_000004.12 4 9111700 - LOC105369250 NNNTTCTGAGAGCAAGAAAG NGG 2 0.095 Tier III
46 NC_000008.11 8 8187087 - ENPP7P1 NNNTTCTGAGAGCAAGAAAG NGG 2 0.095 Tier III
47 NC_000008.11 8 8187087 - FAM85B NNNTTCTGAGAGCAAGAAAG NGG 2 0.095 Tier III
48 NC_000008.11 8 12478567 + FAM86B2-DT NNNTTCTGAGAGCAAGAAAG NGG 2 0.095 Tier III
49 NC_000008.11 8 12478567 + ENPP7P6 NNNTTCTGAGAGCAAGAAAG NGG 2 0.095 Tier III
50 NC_000011.10 11 3467025 + ENPP7P15 NNNTTCTGAGAGCAAGAAAG NGG 2 0.095 Tier III
51 NC_000011.10 11 67838686 + ENPP7P7 NNNTTCTGAGAGCAAGAAAG NGG 2 0.095 Tier III
52 NC_000011.10 11 71755906 - ENPP7P8 NNNTTCTGAGAGCAAGAAAG NGG 2 0.095 Tier III
53 NC_000012.12 12 8275782 + LOC112268090 NNNTTCTGAGAGCAAGAAAG NGG 2 0.095 Tier III
54 NC_000003.12 3 150274330 - LINC01214 NNNTTCTGAGAGCAAGACTG NGG 2 0.095 Tier III
55 NC_000012.12 12 21737758 - LOC102724261 NNNTTCTGTGAGGAAGACAT NGG 2 0.0818 Tier III
56 NC_000012.12 12 21737758 - LOC105369689 NNNTTCTGTGAGGAAGACAT NGG 2 0.0818 Tier III
57 NC_000024.10 Y 2761358 - XGY2 NNNTTCTGAGATCAAGGCAT NGG 2 0.0679 Tier III
58 NC_000024.10 Y 12496394 + XGY1 NNNTTCTGAGATCAAGGCAT NGG 2 0.0679 Tier III
59 NC_000024.10 Y 12496394 + LOC124905301 NNNTTCTGAGATCAAGGCAT NGG 2 0.0679 Tier III
60 NC_000003.12 3 141691027 - LRRC78P NNNTTCTGAGAGCCAGACAT NAG 2 0.0556 Tier III
61 NC_000011.10 11 122030806 - MIR100HG NNNTCCTGAGAGCAAGACAT NTG 2 0.039 Tier III
62 NC_000005.10 5 6819895 - LINC02236 NNNTTCTGAGAGGAAGACAT NAG 2 0.0354 Tier III
63 NC_000002.12 2 36704760 - LOC124905990 NNNTTCTGAGAGGCAGACAT NGG 2 0.0292 Tier III
64 NC_000001.11 1 244108973 - LOC105373261 NNNTTCTGAGAGCAAGACTT NTG 2 0.021 Tier III
65 NC_000009.12 9 124080034 - LOC107987037 NNNTTCTGAGAGCAAGATAT NGC 2 0.0143 Tier III
66 NC_000002.12 2 66014901 + LINC02934 NNNTTCTGAGATCAAGACAT NGT 2 0.0062 Tier III
67 NC_000020.11 20 40982697 - LOC100128988 NNNTTCTGAGAGCAGCACAT NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 50885796 - Stk26 NNNTTCTGAAAGCAAGACGT NGG 2 0.35 Tier I
2 NC_000068.7 2 26209774 - Qsox2 NNNTTCTGAGAGCCAGACCT NGG 2 0.0443 Tier I
3 NC_000070.6 4 128450540 + Csmd2 NNNTTCTGAAAGCAAGACAT NGG 1 0.9333 Tier II
4 NC_000085.6 19 24321466 - Pip5k1b NNNTTCAGAGAGCAAAACAT NGG 2 0.875 Tier II
5 NC_000069.6 3 68177528 - Schip1 NNNTTCTGTGAGCAAGACAT NGG 1 0.6 Tier II
6 NC_000069.6 3 68177528 - Iqschfp NNNTTCTGTGAGCAAGACAT NGG 1 0.6 Tier II
7 NC_000068.7 2 139713535 - Ism1 NNNTTCTAAGAGCTAGACAT NGG 2 0.5333 Tier II
8 NC_000073.6 7 29482322 + Sipa1l3 NNNTTCTGATGGCAAGACAT NGG 2 0.5281 Tier II
9 NC_000081.6 15 79487007 + Kcnj4 NNNTTCTGGGAGCAGGACAT NGG 2 0.4333 Tier II
10 NC_000072.6 6 50396294 + Osbpl3 NNNTTCTGAGAGCTGGACAT NGG 2 0.3467 Tier II
11 NC_000083.6 17 21534379 + Zfp52 NNNTTCTCAGAGCTAGACAT NGG 2 0.3282 Tier II
12 NC_000086.7 X 120431331 + Pcdh11x NNNTTCTGTGACCAAGACAT NGG 2 0.3176 Tier II
13 NC_000086.7 X 85049892 + Dmd NNNCTCTGAGAGCGAGACAT NGG 2 0.302 Tier II
14 NC_000073.6 7 122604466 - Prkcb NNNTTCTAAGAGCCAGACAT NGG 2 0.2143 Tier II
15 NC_000072.6 6 89557950 - Chchd6 NNNTTCTGACACCAAGACAT NGG 2 0.2118 Tier II
16 NC_000073.6 7 135114308 - Dock1 NNNTTGTGAGAGAAAGACAT NGG 2 0.1923 Tier II
17 NC_000068.7 2 39139855 + Scai NNNTTCTGAGTGCTAGACAT NGG 2 0.1641 Tier II
18 NC_000078.6 12 118183172 + Dnah11 NNNTTCTCAGAGCAAGACAT NAG 2 0.1595 Tier II
19 NC_000071.6 5 151413782 - Stard13 NNNTTCTGATAGCAAGACAG NGG 2 0.1434 Tier II
20 NC_000083.6 17 87390511 + Stpg4 NNNTTCTGAGAGCAAGACTT NAG 2 0.1396 Tier II
21 NC_000067.6 1 40625163 - Slc9a4 NNNTTCTTAGAGCATGACAT NGG 2 0.125 Tier II
22 NC_000076.6 10 20679568 + Pde7b NNNTTCAGAGAGCAAGTCAT NGG 2 0.1167 Tier II
23 NC_000074.6 8 122005987 + Banp NNNCTCTGAGAGCAAGCCAT NGG 2 0.1142 Tier II
24 NC_000086.7 X 83438364 + Dmd NNNTTCTGAGAGAAAGACAT NAG 2 0.0997 Tier II
25 NC_000080.6 14 57747235 - Gm52140 NNNTTCTTAGAGCAAGAGAT NGG 2 0.0833 Tier II
26 NC_000080.6 14 38421319 + Nrg3 NNNTTCTCAGAGCAAGACAT NTG 2 0.024 Tier II
27 NC_000075.6 9 29094673 - Ntm NNNTTCTGAGAGGAAGAGAT NGG 2 0.0182 Tier II
28 NC_000083.6 17 38056600 + Olfr129 NNNTTCTAAGAGCAAGACAT NGT 2 0.0161 Tier II
29 NC_000074.6 8 110657947 - Vac14 NNNTTCTGAGAGAAAGACAT NGC 2 0.0085 Tier II
30 NC_000070.6 4 37300100 + Gm12374 NNNTTCTGAAAGCAAGACAT NGG 1 0.9333 Tier III
31 NC_000075.6 9 91324760 - A730094K22Rik NNNTTCTGAGAATAAGACAT NGG 2 0.6533 Tier III
32 NC_000075.6 9 91324760 - Gm40546 NNNTTCTGAGAATAAGACAT NGG 2 0.6533 Tier III
33 NC_000079.6 13 71378952 - Gm40998 NNNGTCAGAGAGCAAGACAT NGG 2 0.5469 Tier III
34 NC_000068.7 2 51083158 + Gm32360 NNNTTCTGAGAACAAGACAA NGG 2 0.525 Tier III
35 NC_000083.6 17 21534379 + 9330136K24Rik NNNTTCTCAGAGCTAGACAT NGG 2 0.3282 Tier III
36 NC_000071.6 5 151413782 - 1700028E10Rik NNNTTCTGATAGCAAGACAG NGG 2 0.1434 Tier III
37 NC_000071.6 5 151413782 - Gm3704 NNNTTCTGATAGCAAGACAG NGG 2 0.1434 Tier III
38 NC_000067.6 1 41573112 + Gm35935 NNNTTCTGAGAGCAAGAAAG NGG 2 0.095 Tier III
39 NC_000076.6 10 3495281 + Gm29987 NNNTTCTGAGAGCAAGAAAG NGG 2 0.095 Tier III
40 NC_000079.6 13 106751135 - Gm30411 NNNTTCTGAGAGCAAGACAG NAG 2 0.0458 Tier III
41 NC_000076.6 10 110858975 - 1700020G17Rik NNNTTCTGAAAGCAACACAT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)