Construct: sgRNA BRDN0001149383

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCTCCCATCCATAGTAAAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80238

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 84474802 + SEMA3A NNNCCCATACACAGTAAAAA NGG 2 0.812 Tier II
2 NC_000002.12 2 98630419 + MGAT4A NNNTCCATACATAGTAAAAA NGG 2 0.6857 Tier II
3 NC_000011.10 11 40929553 + LRRC4C NNNTCCGTCCATAGTAAAAA NGG 2 0.5647 Tier II
4 NC_000010.11 10 125907453 - FANK1 NNNCCCATCCACACTAAAAA NGG 2 0.406 Tier II
5 NC_000009.12 9 19084138 + HAUS6 NNNTCCATCCAGAGTAAAAA NGG 2 0.4 Tier II
6 NC_000005.10 5 38335450 - EGFLAM NNNCCCATCCACAGTAAAGA NGG 2 0.3553 Tier II
7 NC_000006.12 6 132354372 - MOXD1 NNNCACATCCATAGAAAAAA NGG 2 0.3308 Tier II
8 NC_000005.10 5 147622879 + JAKMIP2 NNNCCCATCAATAGTAAAGA NGG 2 0.325 Tier II
9 NC_000005.10 5 55938325 + IL6ST NNNCTGATCCATAGTAAAAA NGG 2 0.3182 Tier II
10 NC_000004.12 4 118303557 + PRSS12 NNNGCCATCCATAGAAAAAA NGG 2 0.2895 Tier II
11 NC_000017.11 17 80039207 + CCDC40 NNNCCCATCCCTACTAAAAA NGG 2 0.1714 Tier II
12 NC_000004.12 4 153171054 - TRIM2 NNNCCCATCCTTACTAAAAA NGG 2 0.1319 Tier II
13 NC_000003.12 3 131936357 - CPNE4 NNNCCCATCCATTGCAAAAA NGG 2 0.0818 Tier II
14 NC_000003.12 3 169172549 + MECOM NNNCCCATACATAGGAAAAA NGG 2 0.0429 Tier II
15 NC_000004.12 4 168952635 - CBR4 NNNCACATCCATAGGAAAAA NGG 2 0.0286 Tier II
16 NC_000007.14 7 50514273 + DDC NNNCACATCCATAGGAAAAA NGG 2 0.0286 Tier II
17 NC_000011.10 11 85423159 - DLG2 NNNCACATCCATAGGAAAAA NGG 2 0.0286 Tier II
18 NC_000012.12 12 10941940 - PRH1 NNNCACATCCATAGGAAAAA NGG 2 0.0286 Tier II
19 NC_000012.12 12 10941940 - PRH1-TAS2R14 NNNCACATCCATAGGAAAAA NGG 2 0.0286 Tier II
20 NC_000023.11 X 118418401 - WDR44 NNNCACATCCATAGGAAAAA NGG 2 0.0286 Tier II
21 NC_000016.10 16 74647770 - RFWD3 NNNCCCATCCCTAGTAAAAA NGC 2 0.0089 Tier II
22 NC_000004.12 4 53163571 + SCFD2 NNNCCCATCCATAGTAAAAA NAT 2 0.0 Tier II
23 NC_000014.9 14 44994297 - TOGARAM1 NNNCCCATCCATAGTAAAAA NAC 2 0.0 Tier II
24 NC_000015.10 15 41730278 + MGA NNNCCCATCCATAGTAAAAA NAC 2 0.0 Tier II
25 NC_000010.11 10 31223586 + LINC02664 NNNACAATCCATAGTAAAAA NGG 2 0.782 Tier III
26 NC_000005.10 5 113649421 + LOC107986441 NNNCCAATCCATAGTAAAAG NGG 2 0.7101 Tier III
27 NC_000014.9 14 76260253 - LOC105370575 NNNCATATCCATAGTAAAAA NGG 2 0.5306 Tier III
28 NC_000002.12 2 167890676 - B3GALT1-AS1 NNNCCCATCCATAGAAAAAG NGG 2 0.4427 Tier III
29 NC_000002.12 2 168384020 + LOC105373734 NNNCACATCCATAGTAAAAG NGG 2 0.437 Tier III
30 NC_000004.12 4 156120423 - LOC102724785 NNNGCCAACCATAGTAAAAA NGG 2 0.4 Tier III
31 NC_000005.10 5 147622879 + JAKMIP2-AS1 NNNCCCATCAATAGTAAAGA NGG 2 0.325 Tier III
32 NC_000012.12 12 11476464 + LOC440084 NNNCCCATCCAAAGTAAAAA NAG 2 0.2074 Tier III
33 NC_000010.11 10 63996283 - LOC124902439 NNNCCCATCCATCCTAAAAA NGG 2 0.0902 Tier III
34 NC_000004.12 4 157824896 + LOC105377509 NNNCACATCCATAGGAAAAA NGG 2 0.0286 Tier III
35 NC_000012.12 12 10941940 - PRH1-PRR4 NNNCACATCCATAGGAAAAA NGG 2 0.0286 Tier III
36 NC_000007.14 7 98268356 + LOC124901705 NNNCCCGTCCATAGTAAAAA NTG 2 0.0275 Tier III
37 NC_000005.10 5 119912711 - LOC105379144 NNNCCCATCCATAGGAGAAA NGG 2 0.0088 Tier III
38 NC_000003.12 3 164181880 - LOC102724419 NNNCCCAGCCATAGTTAAAA NGG 2 0.0 Tier III
39 NC_000022.11 22 45500705 + LOC105373066 NNNCCCATCCAAAGTCAAAA NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 128513813 + Pzp NNNCTCATCCGTAGTAAAAA NGG 2 0.4136 Tier I
2 NC_000084.6 18 45431293 + Kcnn2 NNNTCCATACATAGTAAAAA NGG 2 0.6857 Tier II
3 NC_000078.6 12 52223425 + Nubpl NNNACCTTCCATAGTAAAAA NGG 2 0.3684 Tier II
4 NC_000080.6 14 11938498 - Ptprg NNNCCCATCCATAATAAAGA NGG 2 0.2813 Tier II
5 NC_000081.6 15 80550848 + Enthd1 NNNCCCATCCATGGTAAGAA NGG 2 0.2609 Tier II
6 NC_000074.6 8 61262602 - Sh3rf1 NNNCCAATCCATAGTACAAA NGG 2 0.1639 Tier II
7 NC_000075.6 9 119076337 - Plcd1 NNNCCCATCCATAGTAAGGA NGG 2 0.15 Tier II
8 NC_000079.6 13 18213609 - Pou6f2 NNNCCCATCCATACTAAAAA NAG 2 0.1111 Tier II
9 NC_000073.6 7 143285240 + Kcnq1 NNNCCCACCCATAGGAAAAA NGG 2 0.05 Tier II
10 NC_000075.6 9 81902676 - Mei4 NNNCCCATCCATAGTACAAA NAG 2 0.0458 Tier II
11 NC_000085.6 19 11792719 - Stx3 NNNCCCATCCATTGGAAAAA NGG 2 0.015 Tier II
12 NC_000074.6 8 108376511 - Zfhx3 NNNCCCATCCATAGGAAAAA NGA 2 0.0035 Tier II
13 NC_000075.6 9 82948804 - Phip NNNCCCATCCACAGTCAAAA NGG 2 0.0 Tier II
14 NC_000084.6 18 45431293 + Gm31907 NNNTCCATACATAGTAAAAA NGG 2 0.6857 Tier III
15 NC_000075.6 9 119076337 - Gm51697 NNNCCCATCCATAGTAAGGA NGG 2 0.15 Tier III
16 NC_000075.6 9 73197836 + Gm27211 NNNCCCATACATAGTAAAAA NGA 2 0.0595 Tier III
17 NC_000073.6 7 143285240 + Kcnq1ot1 NNNCCCACCCATAGGAAAAA NGG 2 0.05 Tier III
Download CSV

Other clones with same target sequence:

(none)