Construct: sgRNA BRDN0001149384
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATCTAAGTCCTTAAATGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP5K1B (8395)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77084
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000009.12 |
9 |
68894539 |
- |
PIP5K1B |
NNNCTAAGTCCTTAAATGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000013.11 |
13 |
44463627 |
+ |
TSC22D1 |
NNNCTAACTCCTTAAATGTG |
NGG |
1 |
0.6154 |
Tier II |
| 3 |
NC_000015.10 |
15 |
83278122 |
+ |
BNC1 |
NNNCTAAGTCCTTTAATGTG |
NGG |
1 |
0.5333 |
Tier II |
| 4 |
NC_000010.11 |
10 |
75228975 |
+ |
VDAC2 |
NNNCTAAGTCCTAAAATGTT |
NGG |
2 |
0.4846 |
Tier II |
| 5 |
NC_000015.10 |
15 |
55282959 |
- |
RAB27A |
NNNCTAAGTCAGTAAATGTG |
NGG |
2 |
0.375 |
Tier II |
| 6 |
NC_000004.12 |
4 |
122336093 |
+ |
BLTP1 |
NNNGTAAGGCCTTAAATGTG |
NGG |
2 |
0.3095 |
Tier II |
| 7 |
NC_000009.12 |
9 |
105343769 |
- |
SLC44A1 |
NNNCAAACTCCTTAAATGTG |
NGG |
2 |
0.3077 |
Tier II |
| 8 |
NC_000011.10 |
11 |
134306345 |
- |
GLB1L3 |
NNNCTGAGTCCTTAAATGTC |
NGG |
2 |
0.3061 |
Tier II |
| 9 |
NC_000012.12 |
12 |
65052981 |
+ |
WIF1 |
NNNCTAAGTCCTTGCATGTG |
NGG |
2 |
0.1273 |
Tier II |
| 10 |
NC_000001.11 |
1 |
160747147 |
- |
SLAMF7 |
NNNCTAAATCCTTAAAGGTG |
NGG |
2 |
0.1176 |
Tier II |
| 11 |
NC_000020.11 |
20 |
50940136 |
- |
DPM1 |
NNNCTAGGTCCTTAAATGTG |
NGA |
2 |
0.049 |
Tier II |
| 12 |
NC_000018.10 |
18 |
61430528 |
- |
CDH20 |
NNNCCAAGTCCTTAAATGTG |
NGC |
2 |
0.0222 |
Tier II |
| 13 |
NC_000002.12 |
2 |
109045988 |
+ |
RANBP2 |
NNNCTCAGTCCTTAAATGTG |
NGT |
2 |
0.0073 |
Tier II |
| 14 |
NC_000010.11 |
10 |
60907311 |
+ |
RHOBTB1 |
NNNCTAAGTCCTTAAATGTG |
NAC |
2 |
0.0 |
Tier II |
| 15 |
NC_000012.12 |
12 |
15109856 |
- |
RERG |
NNNCTAAGTCCTTAATTGTG |
NTG |
2 |
0.0 |
Tier II |
| 16 |
NC_000017.11 |
17 |
76489724 |
+ |
RHBDF2 |
NNNTTAAGTCCTTAACTGTG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000013.11 |
13 |
44463627 |
+ |
LOC124903167 |
NNNCTAACTCCTTAAATGTG |
NGG |
1 |
0.6154 |
Tier III |
| 18 |
NC_000009.12 |
9 |
83231910 |
- |
FRMD3-AS1 |
NNNCTAAGTCCTTCAATGTG |
NGG |
1 |
0.2143 |
Tier III |
| 19 |
NC_000020.11 |
20 |
50940136 |
- |
ADNP-AS1 |
NNNCTAGGTCCTTAAATGTG |
NGA |
2 |
0.049 |
Tier III |
| 20 |
NC_000010.11 |
10 |
29406516 |
- |
LOC105376474 |
NNNCTGAGTCCTTAAATGTG |
NTG |
2 |
0.0278 |
Tier III |
| 21 |
NC_000014.9 |
14 |
49091020 |
- |
LOC105378178 |
NNNCTCAGTCCTTAAATGTG |
NTG |
2 |
0.0177 |
Tier III |
| 22 |
NC_000003.12 |
3 |
153648234 |
+ |
LINC02006 |
NNNCTAAGTCCTAAATTGTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
19524786 |
+ |
Nkpd1 |
NNNCTAAGTCCTTAAATTTC |
NGG |
2 |
0.2857 |
Tier I |
| 2 |
NC_000084.6 |
18 |
62690419 |
- |
Spink13 |
NNNCTTAGTCCTTTAATGTG |
NGG |
2 |
0.381 |
Tier II |
| 3 |
NC_000070.6 |
4 |
53524996 |
- |
Slc44a1 |
NNNCTAAGTCCTGAAATGTG |
NGG |
1 |
0.2609 |
Tier II |
| 4 |
NC_000075.6 |
9 |
35094015 |
- |
St3gal4 |
NNNCAATGTCCTTAAATGTG |
NGG |
2 |
0.2188 |
Tier II |
| 5 |
NC_000080.6 |
14 |
124003738 |
+ |
Fgf14 |
NNNCTCAGTCCTTGAATGTG |
NGG |
2 |
0.2121 |
Tier II |
| 6 |
NC_000073.6 |
7 |
138337539 |
- |
Tcerg1l |
NNNCTAAGTCATTAAATGTG |
NAG |
2 |
0.1944 |
Tier II |
| 7 |
NC_000085.6 |
19 |
45945548 |
+ |
Armh3 |
NNNCAAAGTCCTTAAATGTG |
NAG |
2 |
0.1296 |
Tier II |
| 8 |
NC_000083.6 |
17 |
11358763 |
+ |
Prkn |
NNNCTAAGTCCTTAAATGTA |
NGA |
2 |
0.0651 |
Tier II |
| 9 |
NC_000069.6 |
3 |
108075414 |
- |
Ampd2 |
NNNCTAAGTCCTTAAAGGAG |
NGG |
2 |
0.0336 |
Tier II |
| 10 |
NC_000080.6 |
14 |
33961820 |
+ |
Rbp3 |
NNNCTAAGACCTTAAATGTG |
NGT |
2 |
0.015 |
Tier II |
| 11 |
NC_000082.6 |
16 |
25879184 |
- |
Trp63 |
NNNCTAAGTCCTTAAATGTG |
NTT |
2 |
0.0 |
Tier II |
| 12 |
NC_000077.6 |
11 |
96463399 |
+ |
Gm11529 |
NNNCAAAGTCCTTAAAAGTG |
NGG |
2 |
0.2667 |
Tier III |
| 13 |
NC_000080.6 |
14 |
124003738 |
+ |
1700024B18Rik |
NNNCTCAGTCCTTGAATGTG |
NGG |
2 |
0.2121 |
Tier III |
| 14 |
NC_000083.6 |
17 |
44048018 |
+ |
Gm34857 |
NNNCTAAGTCCTTAAAAGTG |
NAG |
2 |
0.1383 |
Tier III |
| 15 |
NC_000074.6 |
8 |
11191132 |
+ |
Gm15418 |
NNNCTAAGTCCTCAAATGTG |
NTG |
2 |
0.0308 |
Tier III |
Other clones with same target sequence:
(none)
