Construct: sgRNA BRDN0001149391

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGTGCCGGGAGAAGAGCACG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAPK2 (23604)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76021

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 63983715 - DAPK2 NNNGCCGGGAGAAGAGCACG NGG 0 1.0 Tier I
2 NC_000005.10 5 152404590 + NMUR2 NNNGCAGGGAGAAGAGCACG NAG 2 0.2407 Tier I
3 NC_000022.11 22 49886507 + ZBED4 NNNGCCGGGAGATGAGCACG NAG 2 0.0778 Tier I
4 NC_000002.12 2 71564144 - DYSF NNNGCCGGGAGAAGAGGACT NGG 2 0.0412 Tier I
5 NC_000002.12 2 101267577 - CNOT11 NNNGCTGGGAGAAGAGCACG NGG 1 0.9286 Tier II
6 NC_000006.12 6 35479635 + TEAD3 NNNGCAGGGAGAAGAGCACA NGG 2 0.8705 Tier II
7 NC_000017.11 17 6118542 + WSCD1 NNNGCTGGGAGAAGAGCACA NGG 2 0.8705 Tier II
8 NC_000017.11 17 65736344 + CEP112 NNNGCTGGGAGAAGAGCAAG NGG 2 0.398 Tier II
9 NC_000009.12 9 37956640 - SHB NNNGCGGGGAGAAGAGCATG NGG 2 0.2308 Tier II
10 NC_000011.10 11 65998093 + EIF1AD NNNGCGGGGAGAACAGCACG NGG 2 0.2143 Tier II
11 NC_000019.10 19 1819428 + REXO1 NNNGCGGGGAGAACAGCACG NGG 2 0.2143 Tier II
12 NC_000022.11 22 25784887 + MYO18B NNNGCCGGGAGAGGTGCACG NGG 2 0.1304 Tier II
13 NC_000020.11 20 61636682 - CDH4 NNNGCTGGGAGAAGAGCAGG NGG 2 0.1161 Tier II
14 NC_000014.9 14 74612020 - LTBP2 NNNGCCGGGAGAACAGCCCG NGG 2 0.0816 Tier II
15 NC_000022.11 22 49886507 + ALG12 NNNGCCGGGAGATGAGCACG NAG 2 0.0778 Tier II
16 NC_000014.9 14 35290838 - PSMA6 NNNTCCGGGAGAAGAGCAGG NGG 2 0.0455 Tier II
17 NC_000002.12 2 241610611 - THAP4 NNNGCCGGGAGAAGACCCCG NGG 2 0.0 Tier II
18 NC_000014.9 14 35290838 - PRORP-PSMA6 NNNTCCGGGAGAAGAGCAGG NGG 2 0.0455 Tier III
19 NC_000023.11 X 74162610 + MKRN5P NNNGCTGGGAGAAGACCACG NGG 2 0.0 Tier III
20 NC_000023.11 X 74162610 + FTX NNNGCTGGGAGAAGACCACG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 66220574 + Dapk2 NNNGCCGGGAGAAGAGCACG NGG 0 1.0 Tier I
2 NC_000083.6 17 43450751 - Adgrf5 NNNGCCGGTAGAAGAGCATG NGG 2 0.2462 Tier I
3 NC_000077.6 11 107703734 + Cacng1 NNNGCAGGGAGAAGAGCAGG NGG 2 0.1161 Tier I
4 NC_000084.6 18 31971575 + Myo7b NNNGCCGGGAGAAGAGCAGG NGC 2 0.0028 Tier I
5 NC_000075.6 9 104594785 - Cpne4 NNNGCCGAGAGAAGAGCACA NGG 2 0.9375 Tier II
6 NC_000068.7 2 31208087 - Gpr107 NNNGCCTGGAGAAGAGCACA NGG 2 0.5357 Tier II
7 NC_000085.6 19 37543296 - Exoc6 NNNGCTGGGAGTAGAGCACG NGG 2 0.3095 Tier II
8 NC_000074.6 8 90348562 - Tox3 NNNGCCGGGAGAGGAGCGCG NGG 2 0.2609 Tier II
9 NC_000077.6 11 88199368 + Mrps23 NNNGCCTGGAGAAGAGCAAG NGG 2 0.2449 Tier II
10 NC_000070.6 4 152270247 + Acot7 NNNGCCGGCAGAAGAGCAAG NGG 2 0.2308 Tier II
11 NC_000068.7 2 140395965 - Macrod2 NNNGCCGGGAGGCGAGCACG NGG 2 0.152 Tier II
12 NC_000067.6 1 59237176 - Als2 NNNGCCGGGAGAAGAGCGCG NAG 2 0.1037 Tier II
13 NC_000071.6 5 136819773 - Col26a1 NNNGCCGGGAGAAGAGAAGG NGG 2 0.0583 Tier II
14 NC_000074.6 8 28593829 - Gm26795 NNNGCCGGGAGAGGAGAACG NGG 2 0.3043 Tier III
15 NC_000085.6 19 58456657 + Gm41876 NNNGCCGGGAGAAGAGGAGG NGG 2 0.0074 Tier III
Download CSV

Other clones with same target sequence:

(none)