Construct: sgRNA BRDN0001149392

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GCCTGAAACAGCATACCAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEK (7010)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75725

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 27203036 + TEK NNNTGAAACAGCATACCAGG NGG 0 1.0 Tier I
2 NC_000005.10 5 32090057 + PDZD2 NNNAGAAACAGCATTCCAGG NGG 2 0.0952 Tier I
3 NC_000008.11 8 63207658 + YTHDF3 NNNTGAAACAGCATAACATG NGG 2 0.6667 Tier II
4 NC_000011.10 11 99622805 - CNTN5 NNNTGAAACAGAATACCATG NGG 2 0.4762 Tier II
5 NC_000007.14 7 72303330 + CALN1 NNNAGAAACTGCATACCAGG NGG 2 0.4202 Tier II
6 NC_000002.12 2 119435785 + TMEM37 NNNTGACACAGAATACCAGG NGG 2 0.3125 Tier II
7 NC_000006.12 6 2113267 - GMDS NNNTGAAACAGGATGCCAGG NGG 2 0.2889 Tier II
8 NC_000015.10 15 59212385 - MYO1E NNNGGAAACACCATACCAGG NGG 2 0.2679 Tier II
9 NC_000005.10 5 75192365 - ANKRD31 NNNTGAAAAAGCATATCAGG NGG 2 0.2637 Tier II
10 NC_000012.12 12 112857785 - RPH3A NNNTGAAAGAGCATATCAGG NGG 2 0.1905 Tier II
11 NC_000005.10 5 96627118 + CAST NNNTGAAATAGCATTCCAGG NGG 2 0.175 Tier II
12 NC_000005.10 5 153704676 - GRIA1 NNNTGGAACAGCATTCCAGG NGG 2 0.1429 Tier II
13 NC_000014.9 14 75831720 - TTLL5 NNNTGAAACAGCATACCTGG NAG 2 0.1296 Tier II
14 NC_000015.10 15 29860235 - TJP1 NNNTGAAACAGGATTCCAGG NGG 2 0.0889 Tier II
15 NC_000005.10 5 59344699 - PDE4D NNNTGAAAAAGCATACGAGG NGG 2 0.0504 Tier II
16 NC_000007.14 7 123742295 - WASL NNNTGAAACAGCATACCAAG NGA 2 0.0496 Tier II
17 NC_000009.12 9 33182503 - B4GALT1 NNNTGATACAGCATACCAGG NCG 2 0.0469 Tier II
18 NC_000004.12 4 102422040 - LOC105377621 NNNTGAATCAGCAAACCAGG NGG 2 0.2653 Tier III
19 NC_000001.11 1 3906461 + LINC01134 NNNTGAAACTGCACACCAGG NGG 2 0.2521 Tier III
20 NC_000005.10 5 96627118 + LOC101929710 NNNTGAAATAGCATTCCAGG NGG 2 0.175 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 105435242 - Cckbr NNNTGAAACAGTAGACCAGG NGG 2 0.0 Tier I
2 NC_000072.6 6 83439491 + Tet3 NNNTGGAACAGCATACCAGG NGG 1 0.7143 Tier II
3 NC_000078.6 12 51917338 + Heatr5a NNNTGAAACAGCATGCCAGG NGG 1 0.65 Tier II
4 NC_000080.6 14 20342838 - Ecd NNNTGAAACAGCATAAAAGG NGG 2 0.4667 Tier II
5 NC_000086.7 X 104604621 - Zdhhc15 NNNTGGAAGAGCATACCAGG NGG 2 0.4422 Tier II
6 NC_000084.6 18 47041260 - Commd10 NNNTGAATCAGCATACCAGG NGG 1 0.4286 Tier II
7 NC_000073.6 7 98924051 - Uvrag NNNTGAAACAGCTTGCCAGG NGG 2 0.195 Tier II
8 NC_000070.6 4 13816588 - Runx1t1 NNNTGAAACAGTTTACCAGG NGG 2 0.1615 Tier II
9 NC_000078.6 12 79674304 - Rad51b NNNTGAAACAGCCAACCAGG NGG 2 0.1303 Tier II
10 NC_000075.6 9 8120532 + Cep126 NNNTGAAACAGCACACCAGG NTG 2 0.0111 Tier II
11 NC_000075.6 9 64208916 + Map2k1 NNNTGAAACAGCATACCTGG NGC 2 0.0111 Tier II
12 NC_000078.6 12 98695886 - Ptpn21 NNNTGAAACAGCATCCCAGG NTG 2 0.0106 Tier II
13 NC_000074.6 8 40331398 - Micu3 NNNTGAAACAGCAGAACAGG NGG 2 0.0 Tier II
14 NC_000070.6 4 114869379 + Gm33655 NNNTGAAAAAGCATACCAAG NGG 2 0.6122 Tier III
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Other clones with same target sequence:

(none)