Construct: sgRNA BRDN0001149393

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTCTTTACGGGTGAAAAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNRK (54861)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76731

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 43303325 + SNRK NNNCTTTACGGGTGAAAAGG NGG 0 1.0 Tier I
2 NC_000018.10 18 50044438 + MYO5B NNNCTTTAGAGGTGAAAAGG NGG 2 0.5778 Tier II
3 NC_000009.12 9 5879491 - BRD10 NNNCTTTAGGGATGAAAAGG NGG 2 0.5778 Tier II
4 NC_000002.12 2 134606974 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
5 NC_000002.12 2 134607355 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
6 NC_000002.12 2 134607798 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
7 NC_000002.12 2 134608610 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
8 NC_000002.12 2 134608898 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
9 NC_000002.12 2 134608963 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
10 NC_000002.12 2 134609090 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
11 NC_000002.12 2 134609248 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
12 NC_000002.12 2 134609313 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
13 NC_000002.12 2 134609378 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
14 NC_000002.12 2 134609446 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
15 NC_000002.12 2 134609511 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
16 NC_000002.12 2 134609579 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
17 NC_000002.12 2 134609613 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
18 NC_000002.12 2 134609647 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
19 NC_000002.12 2 134609681 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
20 NC_000002.12 2 134609715 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
21 NC_000002.12 2 134609749 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
22 NC_000002.12 2 134609783 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
23 NC_000002.12 2 134609817 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
24 NC_000002.12 2 134609851 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
25 NC_000002.12 2 134609885 + TMEM163 NNNCTGTACTGGTGAAAAGG NGG 2 0.4643 Tier II
26 NC_000009.12 9 21853303 + MTAP NNNCTTTAGGGGGGAAAAGG NGG 2 0.1615 Tier II
27 NC_000020.11 20 31640493 + COX4I2 NNNCTTTAAGGGTGAAGAGG NGG 2 0.1513 Tier II
28 NC_000002.12 2 216672378 - IGFBP5 NNNCTTTACTGGTGAAAAGG NTG 2 0.0317 Tier II
29 NC_000008.11 8 141472464 + MROH5 NNNCTTTACAGGTGAAAAGC NGG 2 0.4 Tier III
30 NC_000014.9 14 39486967 + LOC105370460 NNNCTTATCGGGTGAAAAGG NGG 2 0.375 Tier III
31 NC_000014.9 14 39486967 + LOC105370461 NNNCTTATCGGGTGAAAAGG NGG 2 0.375 Tier III
32 NC_000014.9 14 39486967 + LOC112267899 NNNCTTATCGGGTGAAAAGG NGG 2 0.375 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 39805365 - Mrps33 NNNCTTTACAGGTGATAAGG NGG 2 0.0 Tier I
2 NC_000069.6 3 87658591 + Arhgef11 NNNCTTTACAAGTGAAAAGG NGG 2 0.9333 Tier II
3 NC_000081.6 15 37630122 - Ncald NNNCTATATGGGTGAAAAGG NGG 2 0.7583 Tier II
4 NC_000081.6 15 20628933 - Cdh12 NNNATTTACTGGTGAAAAGG NGG 2 0.6842 Tier II
5 NC_000068.7 2 120006784 + Mapkbp1 NNNCTTTACGGATGAGAAGG NGG 2 0.1795 Tier II
6 NC_000077.6 11 60639685 - Gm12625 NNNTTTTACGGATGAAAAGG NGG 2 0.7467 Tier III
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Other clones with same target sequence:

(none)