Construct: sgRNA BRDN0001149400
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTCGGGCCACAAAGTTACT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BLK (640)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75752
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
11549085 |
- |
BLK |
NNNCGGGCCACAAAGTTACT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
31210252 |
+ |
NKAIN1 |
NNNCGGGCCACAAAGCTATT |
NGG |
2 |
0.3077 |
Tier II |
| 3 |
NC_000023.11 |
X |
97847162 |
+ |
ERVFC1 |
NNNAGGGCCACTAAGTTACT |
NGG |
2 |
0.2807 |
Tier II |
| 4 |
NC_000013.11 |
13 |
92483510 |
+ |
GPC5 |
NNNCAGGCCACAAAGTTACT |
NTG |
2 |
0.0338 |
Tier II |
| 5 |
NC_000016.10 |
16 |
80119568 |
- |
LOC105371357 |
NNNCAGGCCACAAAGATACT |
NGG |
2 |
0.7879 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
63383967 |
+ |
Blk |
NNNGGGGCCACAAAGTTGCT |
NGG |
2 |
0.2 |
Tier I |
| 2 |
NC_000071.6 |
5 |
102059273 |
+ |
Wdfy3 |
NNNCGGAACACAAAGTTACT |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000068.7 |
2 |
79666546 |
- |
Itprid2 |
NNNAGGGCCACAAATTTACT |
NGG |
2 |
0.1203 |
Tier II |
| 4 |
NC_000078.6 |
12 |
79864866 |
- |
Gm36132 |
NNNCAGGCCACAAAGTTGCT |
NGG |
2 |
0.3467 |
Tier III |
Other clones with same target sequence:
(none)
