Construct: sgRNA BRDN0001149402
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTCGGGGCAACTTTACAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK33 (65975)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76984
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
8473212 |
- |
STK33 |
NNNCGGGGCAACTTTACAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
43143318 |
- |
PTK7 |
NNNCAGGGCAACTTAACAGA |
NGG |
2 |
0.5018 |
Tier II |
| 3 |
NC_000005.10 |
5 |
76456465 |
+ |
IQGAP2 |
NNNCGGGTCAAATTTACAGA |
NGG |
2 |
0.4464 |
Tier II |
| 4 |
NC_000006.12 |
6 |
118919102 |
+ |
MCM9 |
NNNGGGGGCAACTTTACATA |
NGG |
2 |
0.3333 |
Tier II |
| 5 |
NC_000009.12 |
9 |
114244539 |
+ |
COL27A1 |
NNNCGGGTCAAGTTTACAGA |
NGG |
2 |
0.2778 |
Tier II |
| 6 |
NC_000001.11 |
1 |
197468054 |
- |
CRB1 |
NNNCTGGGAAACTTTACAGA |
NGG |
2 |
0.2571 |
Tier II |
| 7 |
NC_000009.12 |
9 |
117412563 |
+ |
ASTN2 |
NNNCGGGGCACCTATACAGA |
NGG |
2 |
0.2476 |
Tier II |
| 8 |
NC_000011.10 |
11 |
2777329 |
+ |
KCNQ1 |
NNNCAGGGCAACTTTGCAGA |
NGG |
2 |
0.1667 |
Tier II |
| 9 |
NC_000012.12 |
12 |
77607872 |
- |
NAV3 |
NNNCGGGGCAACTTTACAAA |
NTG |
2 |
0.0278 |
Tier II |
| 10 |
NC_000013.11 |
13 |
39014988 |
- |
PROSER1 |
NNNCGGGGCAACTGTACAGA |
NGA |
2 |
0.0 |
Tier II |
| 11 |
NC_000009.12 |
9 |
129068133 |
- |
MIGA2 |
NNNCGGGGCCACTTTCCAGA |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
132368062 |
+ |
Phactr4 |
NNNCAGGGCACCTTTACAGA |
NGG |
2 |
0.3467 |
Tier I |
| 2 |
NC_000075.6 |
9 |
69168980 |
+ |
Rora |
NNNCAAGGCAACTTTACAGA |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000083.6 |
17 |
57026827 |
+ |
Khsrp |
NNNCCAGGCAACTTTACAGA |
NGG |
2 |
0.7857 |
Tier II |
Other clones with same target sequence:
(none)
