Construct: sgRNA BRDN0001149404
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTAGGAGCACTGTCGACCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ACVR1C (130399)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76523
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
157556224 |
+ |
ACVR1C |
NNNAGGAGCACTGTCGACCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
53690743 |
- |
CACNA1D |
NNNAGGAGCACTGTGGACCC |
NAG |
2 |
0.013 |
Tier II |
| 3 |
NC_000017.11 |
17 |
81281226 |
+ |
SLC38A10 |
NNNAGGAGCACTGGAGACCC |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000001.11 |
1 |
39516614 |
- |
BMP8A |
NNNAGGAGCACTGTCCACCT |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000001.11 |
1 |
39769579 |
+ |
BMP8B |
NNNAGGAGCACTGTCCACCT |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000001.11 |
1 |
39769579 |
+ |
OXCT2 |
NNNAGGAGCACTGTCCACCT |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000003.12 |
3 |
63530321 |
- |
SYNPR |
NNNAGGAGCAGTGTCCACCC |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000016.10 |
16 |
2268990 |
+ |
MIR3677HG |
NNNGGGAGCACTGTCGACCA |
NGG |
2 |
0.3125 |
Tier III |
| 9 |
NC_000006.12 |
6 |
130749022 |
- |
LOC105378003 |
NNNAGGAGCACTGCCGACCA |
NGG |
2 |
0.1429 |
Tier III |
| 10 |
NC_000011.10 |
11 |
112288595 |
+ |
LINC02762 |
NNNAGGAGCACTGTGAACCC |
NGG |
2 |
0.05 |
Tier III |
| 11 |
NC_000001.11 |
1 |
39516614 |
- |
OXCT2P1 |
NNNAGGAGCACTGTCCACCT |
NGG |
2 |
0.0 |
Tier III |
| 12 |
NC_000002.12 |
2 |
103995287 |
- |
LINC01965 |
NNNAGGAGCAGTGTCCACCC |
NGG |
2 |
0.0 |
Tier III |
| 13 |
NC_000003.12 |
3 |
63530321 |
- |
SYNPR-AS1 |
NNNAGGAGCAGTGTCCACCC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
45684239 |
- |
Sec1 |
NNNAGGAGCACTGTCGCCCC |
NAG |
2 |
0.0458 |
Tier I |
| 2 |
NC_000070.6 |
4 |
104454176 |
+ |
Dab1 |
NNNAAGAGCAATGTCGACCC |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000080.6 |
14 |
48128379 |
+ |
Peli2 |
NNNAGGAGGACTGTCGACAC |
NGG |
2 |
0.2653 |
Tier II |
| 4 |
NC_000075.6 |
9 |
121249986 |
+ |
Ulk4 |
NNNAGGAGCTCTGCCGACCC |
NGG |
2 |
0.2521 |
Tier II |
| 5 |
NC_000079.6 |
13 |
55153300 |
+ |
Fgfr4 |
NNNAAGAGCACTGTCGCCCC |
NGG |
2 |
0.1529 |
Tier II |
| 6 |
NC_000074.6 |
8 |
23144918 |
- |
Ank1 |
NNNAAGAGCACTGTCGACCC |
NCG |
2 |
0.0929 |
Tier II |
| 7 |
NC_000073.6 |
7 |
45684239 |
- |
Ntn5 |
NNNAGGAGCACTGTCGCCCC |
NAG |
2 |
0.0458 |
Tier II |
| 8 |
NC_000067.6 |
1 |
132625759 |
+ |
Nfasc |
NNNAGGAGCTCTGTGGACCC |
NGG |
2 |
0.0441 |
Tier II |
| 9 |
NC_000070.6 |
4 |
103722780 |
- |
Dab1 |
NNNTGGAGCACTGTGGACCC |
NGG |
2 |
0.0318 |
Tier II |
| 10 |
NC_000080.6 |
14 |
67001836 |
+ |
Bnip3l |
NNNAGGAGCACTGAGGACCC |
NGG |
2 |
0.031 |
Tier II |
| 11 |
NC_000081.6 |
15 |
80005760 |
+ |
Pdgfb |
NNNAGGAGCACTGAGGACCC |
NGG |
2 |
0.031 |
Tier II |
| 12 |
NC_000073.6 |
7 |
134670979 |
- |
Dock1 |
NNNAGGAGCACGGTGGACCC |
NGG |
2 |
0.025 |
Tier II |
| 13 |
NC_000068.7 |
2 |
154254734 |
- |
Cdk5rap1 |
NNNAGGAGCCCTGTTGACCC |
NGG |
2 |
0.0222 |
Tier II |
| 14 |
NC_000068.7 |
2 |
154304087 |
- |
Cdk5rap1 |
NNNAGGAGCCCTGTTGACCC |
NGG |
2 |
0.0222 |
Tier II |
| 15 |
NC_000083.6 |
17 |
46124529 |
+ |
Mrps18a |
NNNAGGAGCACTGTTGGCCC |
NGG |
2 |
0.0118 |
Tier II |
| 16 |
NC_000083.6 |
17 |
46124529 |
+ |
Rsph9 |
NNNAGGAGCACTGTTGGCCC |
NGG |
2 |
0.0118 |
Tier II |
| 17 |
NC_000075.6 |
9 |
60866556 |
+ |
Uaca |
NNNAGGAGCACTGTGGCCCC |
NGG |
2 |
0.0088 |
Tier II |
| 18 |
NC_000076.6 |
10 |
42580128 |
+ |
Nr2e1 |
NNNAGGAGCACTGTCGCCCC |
NGC |
2 |
0.0039 |
Tier II |
| 19 |
NC_000078.6 |
12 |
117622794 |
- |
Rapgef5 |
NNNAGGAGCACAGTCTACCC |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000077.6 |
11 |
62985453 |
- |
Cdrt4 |
NNNAGGAGCACTGGCTACCC |
NGG |
2 |
0.0 |
Tier II |
| 21 |
NC_000069.6 |
3 |
54633670 |
+ |
B020017C02Rik |
NNNAAGAGCACTGCCGACCC |
NGG |
2 |
0.2476 |
Tier III |
| 22 |
NC_000074.6 |
8 |
23144918 |
- |
Gm15816 |
NNNAAGAGCACTGTCGACCC |
NCG |
2 |
0.0929 |
Tier III |
| 23 |
NC_000075.6 |
9 |
12231229 |
+ |
Gm29785 |
NNNAGGAGCAGTGTCGCCCC |
NGG |
2 |
0.0441 |
Tier III |
| 24 |
NC_000075.6 |
9 |
60866556 |
+ |
Gm33643 |
NNNAGGAGCACTGTGGCCCC |
NGG |
2 |
0.0088 |
Tier III |
| 25 |
NC_000074.6 |
8 |
10736540 |
+ |
Gm30666 |
NNNAGGAGCACTGGAGACCC |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
