Construct: sgRNA BRDN0001149409
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCAGTATGTCCTGTCCCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK35 (140901)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77354
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
2116761 |
+ |
STK35 |
NNNAGTATGTCCTGTCCCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
120874598 |
- |
PHF19 |
NNNAGTATGTGCTGTGCCGG |
NGG |
2 |
0.0385 |
Tier I |
| 3 |
NC_000007.14 |
7 |
14675227 |
- |
DGKB |
NNNAGTATGTCCTGCCCCTG |
NGG |
2 |
0.1818 |
Tier II |
| 4 |
NC_000014.9 |
14 |
68922462 |
+ |
ACTN1 |
NNNAGTATTTCCTGTCCGGG |
NGG |
2 |
0.0711 |
Tier II |
| 5 |
NC_000024.10 |
Y |
15548808 |
+ |
STSP1 |
NNNAGTATTTCCTGTCCCTG |
NGG |
2 |
0.3556 |
Tier III |
| 6 |
NC_000011.10 |
11 |
27132485 |
+ |
BBOX1-AS1 |
NNNAGTAGGTCCTGTCCCGG |
NTG |
2 |
0.0286 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
129810583 |
+ |
Stk35 |
NNNAGTATGTCCTGTCCCGG |
NGA |
1 |
0.0694 |
Tier I |
| 2 |
NC_000067.6 |
1 |
89133712 |
- |
Sh3bp4 |
NNNACTATGTCCTGTCCCGA |
NGG |
2 |
0.7366 |
Tier II |
| 3 |
NC_000077.6 |
11 |
55010876 |
- |
Anxa6 |
NNNAGTGTGTCCTGTCCCAG |
NGG |
2 |
0.5042 |
Tier II |
| 4 |
NC_000076.6 |
10 |
85458585 |
+ |
Btbd11 |
NNNGGTCTGTCCTGTCCCGG |
NGG |
2 |
0.2734 |
Tier II |
| 5 |
NC_000069.6 |
3 |
129625581 |
+ |
Elovl6 |
NNNAGTATGTACTGTCCCGG |
NAG |
2 |
0.1944 |
Tier II |
| 6 |
NC_000083.6 |
17 |
42860644 |
- |
Cd2ap |
NNNAGTATGGGCTGTCCCGG |
NGG |
2 |
0.125 |
Tier II |
Other clones with same target sequence:
(none)
