Construct: sgRNA BRDN0001149411
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCAGAACGTGCAGTTCTCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK32C (282974)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76933
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
132226874 |
- |
STK32C |
NNNGAACGTGCAGTTCTCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
139316836 |
- |
LOC124909439 |
NNNGAACTTGCAGTTCTCCT |
NGG |
2 |
0.4375 |
Tier I |
| 3 |
NC_000012.12 |
12 |
4370768 |
+ |
FGF23 |
NNNGAACCTGCAGTTCTCCG |
NGT |
2 |
0.0099 |
Tier I |
| 4 |
NC_000016.10 |
16 |
4880191 |
+ |
UBN1 |
NNNGAACGTGCAGGTCTCCC |
NGG |
2 |
0.0 |
Tier I |
| 5 |
NC_000004.12 |
4 |
3530445 |
- |
LRPAP1 |
NNNGAACGTGAAGTTCTTCG |
NGG |
2 |
0.4821 |
Tier II |
| 6 |
NC_000011.10 |
11 |
11560500 |
+ |
GALNT18 |
NNNGAACGTGCAGATCTCCT |
NGG |
2 |
0.4333 |
Tier II |
| 7 |
NC_000010.11 |
10 |
5911338 |
+ |
FBH1 |
NNNGAACGTGCAGTTCTGAG |
NGG |
2 |
0.0571 |
Tier II |
| 8 |
NC_000001.11 |
1 |
3171083 |
- |
PRDM16 |
NNNGAAGGTGCAGGTCTCCG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000009.12 |
9 |
99710263 |
+ |
LOC101928438 |
NNNGAACATGCAGTTCTCCT |
NGG |
2 |
0.7 |
Tier III |
| 10 |
NC_000009.12 |
9 |
99710263 |
+ |
LOC124902234 |
NNNGAACATGCAGTTCTCCT |
NGG |
2 |
0.7 |
Tier III |
| 11 |
NC_000001.11 |
1 |
168773239 |
- |
LOC124904452 |
NNNGAACGTGCAGTTCTCCC |
NGC |
2 |
0.0095 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
28973957 |
+ |
Cfap77 |
NNNGAATGTGCAGTTCTCCA |
NGG |
2 |
0.7617 |
Tier II |
| 2 |
NC_000071.6 |
5 |
120612017 |
- |
Rita1 |
NNNGATCGTGCAGTTCTCCG |
NGG |
1 |
0.7143 |
Tier II |
| 3 |
NC_000070.6 |
4 |
103355830 |
+ |
Oma1 |
NNNGAACGTGCTGTTCTCCT |
NGG |
2 |
0.2333 |
Tier II |
| 4 |
NC_000074.6 |
8 |
126873469 |
- |
Gm31718 |
NNNGAAGGTGCAGTTCTTCG |
NGG |
2 |
0.3025 |
Tier III |
Other clones with same target sequence:
(none)
