Construct: sgRNA BRDN0001149418
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGGCTCGCAAGTACTTTGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DYRK1B (9149)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76621
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
39827339 |
- |
DYRK1B |
NNNCTCGCAAGTACTTTGAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
94546688 |
+ |
HELLS |
NNNTTCGCAAGTGCTTTGAA |
NGG |
2 |
0.5217 |
Tier II |
| 3 |
NC_000010.11 |
10 |
14850841 |
+ |
HSPA14 |
NNNGTGGCAAGTACTTTGAA |
NGG |
2 |
0.25 |
Tier II |
| 4 |
NC_000013.11 |
13 |
112765040 |
+ |
ATP11A |
NNNCTTGCAAGTCCTTTGAA |
NGG |
2 |
0.1955 |
Tier II |
| 5 |
NC_000001.11 |
1 |
15099663 |
- |
KAZN |
NNNATCGCAAGTCCTTTGAA |
NGG |
2 |
0.1773 |
Tier II |
| 6 |
NC_000021.9 |
21 |
36814222 |
- |
HLCS |
NNNCTGGCAAGTACCTTGAA |
NGG |
2 |
0.1364 |
Tier II |
| 7 |
NC_000014.9 |
14 |
35606145 |
- |
RALGAPA1 |
NNNCTCGTAAGTACTTTGAA |
NGA |
2 |
0.0608 |
Tier II |
| 8 |
NC_000002.12 |
2 |
20643166 |
+ |
HS1BP3 |
NNNCTCGCAAGTACTTTCAA |
NTG |
2 |
0.0186 |
Tier II |
| 9 |
NC_000003.12 |
3 |
8015542 |
+ |
LOC101927394 |
NNNCTCGCAGATACTTTGAA |
NGG |
2 |
0.5556 |
Tier III |
| 10 |
NC_000023.11 |
X |
96538612 |
- |
LOC107985714 |
NNNCTCGCAGGCACTTTGAA |
NGG |
2 |
0.5263 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
28185383 |
+ |
Dyrk1b |
NNNCTCGAAAGTACTTTGAG |
NGG |
2 |
0.4971 |
Tier I |
| 2 |
NC_000067.6 |
1 |
124573692 |
+ |
Dpp10 |
NNNATAGCAAGTACTTTGAA |
NGG |
2 |
0.782 |
Tier II |
| 3 |
NC_000077.6 |
11 |
108504552 |
+ |
Cep112 |
NNNCTCGCCAGTACTTTGAA |
NGG |
1 |
0.5714 |
Tier II |
| 4 |
NC_000083.6 |
17 |
10473114 |
+ |
Pacrg |
NNNCTTGCAGGTACTTTGAA |
NGG |
2 |
0.5159 |
Tier II |
| 5 |
NC_000071.6 |
5 |
64102959 |
- |
Pgm2 |
NNNCTGACAAGTACTTTGAA |
NGG |
2 |
0.5 |
Tier II |
| 6 |
NC_000067.6 |
1 |
57905595 |
+ |
Spats2l |
NNNCTCCCAAGTACTCTGAA |
NGG |
2 |
0.4583 |
Tier II |
| 7 |
NC_000072.6 |
6 |
96863726 |
- |
Tafa4 |
NNNCTCTCTAGTACTTTGAA |
NGG |
2 |
0.3429 |
Tier II |
| 8 |
NC_000078.6 |
12 |
44919760 |
- |
Stxbp6 |
NNNCTCCCAATTACTTTGAA |
NGG |
2 |
0.2644 |
Tier II |
| 9 |
NC_000072.6 |
6 |
93716955 |
- |
Magi1 |
NNNCTCGCAACTCCTTTGAA |
NGG |
2 |
0.0902 |
Tier II |
| 10 |
NC_000073.6 |
7 |
110905152 |
+ |
Mrvi1 |
NNNCTGGCAAGTACTTTGAA |
NTG |
2 |
0.0195 |
Tier II |
| 11 |
NC_000083.6 |
17 |
10473114 |
+ |
A230009B12Rik |
NNNCTTGCAGGTACTTTGAA |
NGG |
2 |
0.5159 |
Tier III |
| 12 |
NC_000073.6 |
7 |
92642524 |
- |
Gm26944 |
NNNCTAGCAAGTACTTAGAA |
NGG |
2 |
0.4952 |
Tier III |
| 13 |
NC_000073.6 |
7 |
110905152 |
+ |
Gm16336 |
NNNCTGGCAAGTACTTTGAA |
NTG |
2 |
0.0195 |
Tier III |
Other clones with same target sequence:
(none)
