GPP Web Portal - Clone Details

Construct: sgRNA BRDN0001149423

Construct Description:

Construct Type:: sgRNA
Other Identifiers:: n/a
DNA Barcode:: GAACTCAACCAGAGGGCCAA

Original Target:

Taxon:: Homo sapiens (human)
Gene:: NO_SITE control
CRISPR Mechanism: CRISPRko

Vector Information:

Vector Backbone:: pXPR_003
Pol II Cassette 1:: EF1A-PuroR
Pol II Cassette 2:: n/a
Selection Marker:: PuroR
Visible Reporter:: n/a
Epitope Tag:: n/a

Additional Information:

Addgene ID:: 80255

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000005.10	5	179825218	-	SQSTM1	NNNCTCACCCAGAGGGCTAA	NGG	2	0.2755	Tier I
2	NC_000007.14	7	157568907	-	PTPRN2	NNNCTCAACAAGATGGCCAA	NGG	2	0.2311	Tier I
3	NC_000003.12	3	30810463	-	GADL1	NNNATCAACCAAAGGGCCAA	NGG	2	0.786	Tier II
4	NC_000002.12	2	202161390	-	KIAA2012	NNNTTTAACCAGAGGGCCAA	NGG	2	0.7429	Tier II
5	NC_000002.12	2	94591583	-	AQP7B	NNNCTCTACCAGAGGGCCAT	NGG	2	0.2625	Tier II
6	NC_000009.12	9	33398357	+	AQP7	NNNCTCTACCAGAGGGCCAT	NGG	2	0.2625	Tier II
7	NC_000003.12	3	126397887	+	CFAP100	NNNCTCAACCAGAGGGCCCA	NGG	1	0.2069	Tier II
8	NC_000003.12	3	45134825	+	CDCP1	NNNCTCACCCAGAGGGCCCA	NGG	2	0.0887	Tier II
9	NC_000008.11	8	143440867	+	ZC3H3	NNNCTCAACCAGAGGGCCCC	NGG	2	0.047	Tier II
10	NC_000015.10	15	80462142	+	ARNT2	NNNCTCAAGCAGAGGGCCAA	NGC	2	0.0138	Tier II
11	NC_000014.9	14	74511200	+	LTBP2	NNNCTCAACCAGAGGTCCCA	NGG	2	0.0	Tier II
12	NC_000012.12	12	22585775	+	C2CD5-AS1	NNNCTTAACTAGAGGGCCAA	NGG	2	0.8739	Tier III
13	NC_000009.12	9	40494518	+	AQP7P5	NNNCTCTACCAGAGGGCCAT	NGG	2	0.2625	Tier III
14	NC_000009.12	9	40494518	+	LOC124902160	NNNCTCTACCAGAGGGCCAT	NGG	2	0.2625	Tier III
15	NC_000009.12	9	43122844	+	LOC102724922	NNNCTCTACCAGAGGGCCAT	NGG	2	0.2625	Tier III
16	NC_000009.12	9	63130434	-	LOC105379814	NNNCTCTACCAGAGGGCCAT	NGG	2	0.2625	Tier III
17	NC_000009.12	9	63330018	+	AQP7P1	NNNCTCTACCAGAGGGCCAT	NGG	2	0.2625	Tier III
18	NC_000009.12	9	64634522	+	AQP7P2	NNNCTCTACCAGAGGGCCAT	NGG	2	0.2625	Tier III
19	NC_000009.12	9	65139736	+	LOC102724507	NNNCTCTACCAGAGGGCCAT	NGG	2	0.2625	Tier III
20	NC_000009.12	9	65881831	-	AQP7P3	NNNCTCTACCAGAGGGCCAT	NGG	2	0.2625	Tier III
21	NC_000009.12	9	65881831	-	LOC112267859	NNNCTCTACCAGAGGGCCAT	NGG	2	0.2625	Tier III
22	NC_000004.12	4	163177415	+	LOC105377517	NNNGTCACCCAGAGGGCCAA	NGG	2	0.2143	Tier III
23	NC_000011.10	11	90627752	-	DISC1FP1	NNNCTCAACCGGTGGGCCAA	NGG	2	0.195	Tier III
24	NC_000006.12	6	4018611	-	LOC124901245	NNNCTCAACCTGAGGGCCAC	NGG	2	0.0699	Tier III
25	NC_000009.12	9	81410407	+	RPS20P25	NNNCTCAACCAGAGGCACAA	NGG	2	0.0	Tier III

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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

	Reference Sequence	Chromosome	Cut Position	Match Strand	Match Gene	Match Sequence	PAM Seq.	Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases.	CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016.	Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works.
1	NC_000068.7	2	130281536	-	Idh3b	NNNCTCACCCAGAGCGCCAA	NGG	2	0.1169	Tier I
2	NC_000081.6	15	100641477	-	Bin2	NNNCTCAAGCAGAGGGCCAA	NGG	1	0.619	Tier II
3	NC_000074.6	8	119821636	+	Cotl1	NNNGTCAACCAGAGAGCCAA	NGG	2	0.4706	Tier II
4	NC_000068.7	2	118834506	-	Knstrn	NNNCTCAACCAGAGAGACAA	NGG	2	0.4392	Tier II
5	NC_000076.6	10	78591188	-	Syde1	NNNCTGAAACAGAGGGCCAA	NGG	2	0.4286	Tier II
6	NC_000075.6	9	117049837	-	Rbms3	NNNCTCAACCAGACAGCCAA	NGG	2	0.4034	Tier II
7	NC_000081.6	15	89496664	+	Shank3	NNNGTCAACCAGAGGGCCAG	NGG	2	0.3824	Tier II
8	NC_000072.6	6	47015421	+	Cntnap2	NNNCTCACCCAGAAGGCCAA	NGG	2	0.3214	Tier II
9	NC_000067.6	1	135636788	+	Nav1	NNNCTGAACCAGAGGGACAA	NGG	2	0.2333	Tier II
10	NC_000067.6	1	89528186	-	Agap1	NNNCACACCCAGAGGGCCAA	NGG	2	0.2143	Tier II
11	NC_000081.6	15	79914879	+	Apobec3	NNNCACAACCAGTGGGCCAA	NGG	2	0.15	Tier II
12	NC_000067.6	1	106212984	-	Phlpp1	NNNCTCAACCACAGCGCCAA	NGG	2	0.1444	Tier II
13	NC_000071.6	5	72701677	+	Txk	NNNCTCTACCAGAGGGCCCA	NGG	2	0.0905	Tier II
14	NC_000075.6	9	52141329	-	Zc3h12c	NNNCTCAACCTGAGGGCCAC	NGG	2	0.0699	Tier II
15	NC_000067.6	1	135349511	-	Lmod1	NNNCCCAACCAGAGGTCCAA	NGG	2	0.0	Tier II
16	NC_000075.6	9	113057903	+	Gm36251	NNNCTCAGTCAGAGGGCCAA	NGG	2	0.6417	Tier III
17	NC_000070.6	4	38707746	+	Gm12381	NNNTTCAACCAGAGGGCCTA	NGG	2	0.4308	Tier III
18	NC_000072.6	6	47015421	+	Gm35875	NNNCTCACCCAGAAGGCCAA	NGG	2	0.3214	Tier III

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Other clones with same target sequence:

(none)