Construct: sgRNA BRDN0001149426

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTTCTCACCATATAGCCCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K1 (4214)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77824

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000005.10 5 56864801 + MAP3K1 NNNCTCACCATATAGCCCTG NGG 0 1.0 Tier I
2 NC_000001.11 1 35457185 - KIAA0319L NNNCTCACTATATAGCCCTT NGG 2 0.6125 Tier II
3 NC_000002.12 2 31377312 - XDH NNNCTCACCTCATAGCCCTG NGG 2 0.5882 Tier II
4 NC_000019.10 19 47807764 + TPRX1 NNNCTCAGCATATTGCCCTG NGG 2 0.3429 Tier II
5 NC_000006.12 6 42185252 + GUCA1B NNNCTCCCCACATAGCCCTG NGG 2 0.2917 Tier II
6 NC_000002.12 2 105886725 - NCK2 NNNCTCACCATATAGACCTG NAG 2 0.2593 Tier II
7 NC_000001.11 1 118074265 - SPAG17 NNNCTCACCATACAGCCCTG NAG 2 0.2047 Tier II
8 NC_000011.10 11 11454853 + GALNT18 NNNCTCACCATCAAGCCCTG NGG 2 0.1822 Tier II
9 NC_000011.10 11 11306025 + GALNT18 NNNCTCTCCATATAGCCCAG NGG 2 0.125 Tier II
10 NC_000016.10 16 27735455 + KATNIP NNNCTCACCAGATATCCCTG NGG 2 0.0571 Tier II
11 NC_000007.14 7 107939087 - LAMB1 NNNCTCACCATCTAGGCCTG NGG 2 0.0405 Tier II
12 NC_000005.10 5 149627583 - ARHGEF37 NNNCTCTCCATATAGCCCTG NTG 2 0.017 Tier II
13 NC_000007.14 7 55241809 + ELDR NNNGCCACCATATAGCCCTG NGG 2 0.5 Tier III
14 NC_000001.11 1 116454189 - LINC01762 NNNCTCACCATAGAGCCATG NGG 2 0.1405 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 122713193 - Nanog NNNTTCACCAGATAGCCCTG NGG 2 0.32 Tier I
2 NC_000071.6 5 24589808 + Chpf2 NNNCTCACCTTACAGCCCTG NGG 2 0.6966 Tier II
3 NC_000078.6 12 72132264 - Ccdc175 NNNCTTACCATGTAGCCCTG NGG 2 0.6706 Tier II
4 NC_000067.6 1 153433730 - Shcbp1l NNNCTCACTATGTAGCCCTG NGG 2 0.6319 Tier II
5 NC_000085.6 19 56293127 + Habp2 NNNCTCACTATGTAGCCCTG NGG 2 0.6319 Tier II
6 NC_000077.6 11 87868077 + Epx NNNCTCACCAAACAGCCCTG NGG 2 0.5921 Tier II
7 NC_000077.6 11 81125081 + Asic2 NNNTTCACCACATAGCCCTG NGG 2 0.5333 Tier II
8 NC_000074.6 8 61298975 - Sh3rf1 NNNCTCACCACACAGCCCTG NGG 2 0.5263 Tier II
9 NC_000079.6 13 44842936 - Jarid2 NNNCTCACCAAAAAGCCCTG NGG 2 0.5192 Tier II
10 NC_000073.6 7 47078550 - Ptpn5 NNNCACACCATATAGACCTG NGG 2 0.5 Tier II
11 NC_000071.6 5 48307743 + Slit2 NNNCTCACAATATTGCCCTG NGG 2 0.4571 Tier II
12 NC_000074.6 8 105914883 - Pskh1 NNNCTCACTATATAGCACTG NGG 2 0.4083 Tier II
13 NC_000067.6 1 155284635 + Xpr1 NNNCTCACTAGATAGCCCTG NGG 2 0.35 Tier II
14 NC_000086.7 X 94260452 - Klhl15 NNNCTCATCATATAGCCCAG NGG 2 0.25 Tier II
15 NC_000068.7 2 155492944 + Ggt7 NNNCTCACCATCAAGCCCTG NGG 2 0.1822 Tier II
16 NC_000070.6 4 123923323 + Rragc NNNCTCACGATATAGCCCTG NAG 2 0.1605 Tier II
17 NC_000067.6 1 171243169 - Ndufs2 NNNCTCACCATATAGCCATG NAG 2 0.1396 Tier II
18 NC_000068.7 2 70212940 + Myo3b NNNCTGACCATCTAGCCCTG NGG 2 0.1316 Tier II
19 NC_000083.6 17 67774897 - Lama1 NNNGTCACCATCTAGCCCTG NGG 2 0.1316 Tier II
20 NC_000068.7 2 165610487 - Eya2 NNNCTCACCATAGAGCACTG NGG 2 0.1217 Tier II
21 NC_000083.6 17 26759589 - Crebrf NNNCTCTCCATCTAGCCCTG NGG 2 0.1151 Tier II
22 NC_000073.6 7 47350362 - Mrgpra1 NNNCTCACCATGTAGCCCTG NCG 2 0.0774 Tier II
23 NC_000082.6 16 17702116 + Med15 NNNCTCACCATATAGCCTTG NCG 2 0.0689 Tier II
24 NC_000086.7 X 147417785 - Il13ra2 NNNCTCACCATGTAGCCCTG NGA 2 0.0502 Tier II
25 NC_000070.6 4 44853759 + Zcchc7 NNNCTCACTATATAGCCCTG NTG 2 0.0341 Tier II
26 NC_000072.6 6 145003036 + Bcat1 NNNCTCACTATATAGCCCTG NTG 2 0.0341 Tier II
27 NC_000073.6 7 116274122 - Plekha7 NNNCTCACTATATAGCCCTG NTG 2 0.0341 Tier II
28 NC_000067.6 1 86241835 + Armc9 NNNCTCACTATATAGCCCTG NGC 2 0.0194 Tier II
29 NC_000069.6 3 132802640 + Tbck NNNCTCACTATATAGCCCTG NGC 2 0.0194 Tier II
30 NC_000073.6 7 99464675 + Klhl35 NNNCTCACTATATAGCCCTG NGC 2 0.0194 Tier II
31 NC_000070.6 4 132112204 + Oprd1 NNNCTCACCATGTAGCCCTG NGC 2 0.016 Tier II
32 NC_000075.6 9 64717839 - Rab11a NNNCTCACTATATAGCCCTG NGT 2 0.0141 Tier II
33 NC_000082.6 16 4316878 + Adcy9 NNNCTCACTATATAGCCCTG NGT 2 0.0141 Tier II
34 NC_000077.6 11 80162739 - Adap2 NNNCTCACCATACAGCCCTG NGT 2 0.0127 Tier II
35 NC_000076.6 10 94906017 - Plxnc1 NNNCACACCATATAGCCCTG NGC 2 0.0111 Tier II
36 NC_000084.6 18 32102600 - Iws1 NNNCTCACCATATAGCCTTG NGT 2 0.0104 Tier II
37 NC_000086.7 X 102540355 + Phka1 NNNCTCAGCATATAGCCCTG NGT 2 0.0104 Tier II
38 NC_000073.6 7 16280204 - Ccdc9 NNNCTCACCATCTAGCCCTG NTG 2 0.0103 Tier II
39 NC_000073.6 7 101206174 + Fchsd2 NNNCTCACCATATAGTCCTG NGT 2 0.005 Tier II
40 NC_000070.6 4 119335783 + Ccdc30 NNNCTCACCATATAGCCCTG NCT 2 0.0 Tier II
41 NC_000074.6 8 61873964 + Palld NNNCTCACCATATAGCCCTG NCT 2 0.0 Tier II
42 NC_000074.6 8 104370703 + Cmtm4 NNNCTCACCATATAGCCCTG NCT 2 0.0 Tier II
43 NC_000075.6 9 45218368 - BC049352 NNNCTCACCATATAGCCCTG NCT 2 0.0 Tier II
44 NC_000076.6 10 126877162 + Atp23 NNNCTCACCATATAGCCCTG NAT 2 0.0 Tier II
45 NC_000076.6 10 128103401 - Baz2a NNNCTCACCATATAGCCCTG NCA 2 0.0 Tier II
46 NC_000079.6 13 58595741 - Slc28a3 NNNCTCACCATATAGCCCTG NCT 2 0.0 Tier II
47 NC_000083.6 17 27064973 - Itpr3 NNNCTCACCATATAGCCCTG NCT 2 0.0 Tier II
48 NC_000073.6 7 81531118 - 2900076A07Rik NNNCTCACTATGTAGCCCTG NGG 2 0.6319 Tier III
49 NC_000080.6 14 25393419 + Zmiz1os1 NNNCTGACCATATAACCCTG NGG 2 0.4706 Tier III
50 NC_000085.6 19 10785432 + A430093F15Rik NNNCTCTCCATCTAGCCCTG NGG 2 0.1151 Tier III
51 NC_000067.6 1 31032304 + Gm28644 NNNCTCACTATATAGCCCTG NGT 2 0.0141 Tier III
52 NC_000067.6 1 31032304 + Gm29669 NNNCTCACTATATAGCCCTG NGT 2 0.0141 Tier III
53 NC_000077.6 11 29634924 - Gm12092 NNNCTCACTATATAGCCCTG NGT 2 0.0141 Tier III
54 NC_000086.7 X 140528407 + Gm39538 NNNCTCACCATGTAGCCCTG NGT 2 0.0116 Tier III
55 NC_000067.6 1 180547396 - Gm31986 NNNCTCACCATATAGCCCTG NCT 2 0.0 Tier III
56 NC_000081.6 15 54961887 - Gm26684 NNNCTCACCATATAGCCCTG NCT 2 0.0 Tier III
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Other clones with same target sequence:

(none)