Construct: sgRNA BRDN0001149426
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTCTCACCATATAGCCCTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- MAP3K1 (4214)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000005.10 | 5 | 56864801 | + | MAP3K1 | NNNCTCACCATATAGCCCTG | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 35457185 | - | KIAA0319L | NNNCTCACTATATAGCCCTT | NGG | 2 | 0.6125 | Tier II |
3 | NC_000002.12 | 2 | 31377312 | - | XDH | NNNCTCACCTCATAGCCCTG | NGG | 2 | 0.5882 | Tier II |
4 | NC_000019.10 | 19 | 47807764 | + | TPRX1 | NNNCTCAGCATATTGCCCTG | NGG | 2 | 0.3429 | Tier II |
5 | NC_000006.12 | 6 | 42185252 | + | GUCA1B | NNNCTCCCCACATAGCCCTG | NGG | 2 | 0.2917 | Tier II |
6 | NC_000002.12 | 2 | 105886725 | - | NCK2 | NNNCTCACCATATAGACCTG | NAG | 2 | 0.2593 | Tier II |
7 | NC_000001.11 | 1 | 118074265 | - | SPAG17 | NNNCTCACCATACAGCCCTG | NAG | 2 | 0.2047 | Tier II |
8 | NC_000011.10 | 11 | 11454853 | + | GALNT18 | NNNCTCACCATCAAGCCCTG | NGG | 2 | 0.1822 | Tier II |
9 | NC_000011.10 | 11 | 11306025 | + | GALNT18 | NNNCTCTCCATATAGCCCAG | NGG | 2 | 0.125 | Tier II |
10 | NC_000016.10 | 16 | 27735455 | + | KATNIP | NNNCTCACCAGATATCCCTG | NGG | 2 | 0.0571 | Tier II |
11 | NC_000007.14 | 7 | 107939087 | - | LAMB1 | NNNCTCACCATCTAGGCCTG | NGG | 2 | 0.0405 | Tier II |
12 | NC_000005.10 | 5 | 149627583 | - | ARHGEF37 | NNNCTCTCCATATAGCCCTG | NTG | 2 | 0.017 | Tier II |
13 | NC_000007.14 | 7 | 55241809 | + | ELDR | NNNGCCACCATATAGCCCTG | NGG | 2 | 0.5 | Tier III |
14 | NC_000001.11 | 1 | 116454189 | - | LINC01762 | NNNCTCACCATAGAGCCATG | NGG | 2 | 0.1405 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000072.6 | 6 | 122713193 | - | Nanog | NNNTTCACCAGATAGCCCTG | NGG | 2 | 0.32 | Tier I |
2 | NC_000071.6 | 5 | 24589808 | + | Chpf2 | NNNCTCACCTTACAGCCCTG | NGG | 2 | 0.6966 | Tier II |
3 | NC_000078.6 | 12 | 72132264 | - | Ccdc175 | NNNCTTACCATGTAGCCCTG | NGG | 2 | 0.6706 | Tier II |
4 | NC_000067.6 | 1 | 153433730 | - | Shcbp1l | NNNCTCACTATGTAGCCCTG | NGG | 2 | 0.6319 | Tier II |
5 | NC_000085.6 | 19 | 56293127 | + | Habp2 | NNNCTCACTATGTAGCCCTG | NGG | 2 | 0.6319 | Tier II |
6 | NC_000077.6 | 11 | 87868077 | + | Epx | NNNCTCACCAAACAGCCCTG | NGG | 2 | 0.5921 | Tier II |
7 | NC_000077.6 | 11 | 81125081 | + | Asic2 | NNNTTCACCACATAGCCCTG | NGG | 2 | 0.5333 | Tier II |
8 | NC_000074.6 | 8 | 61298975 | - | Sh3rf1 | NNNCTCACCACACAGCCCTG | NGG | 2 | 0.5263 | Tier II |
9 | NC_000079.6 | 13 | 44842936 | - | Jarid2 | NNNCTCACCAAAAAGCCCTG | NGG | 2 | 0.5192 | Tier II |
10 | NC_000073.6 | 7 | 47078550 | - | Ptpn5 | NNNCACACCATATAGACCTG | NGG | 2 | 0.5 | Tier II |
11 | NC_000071.6 | 5 | 48307743 | + | Slit2 | NNNCTCACAATATTGCCCTG | NGG | 2 | 0.4571 | Tier II |
12 | NC_000074.6 | 8 | 105914883 | - | Pskh1 | NNNCTCACTATATAGCACTG | NGG | 2 | 0.4083 | Tier II |
13 | NC_000067.6 | 1 | 155284635 | + | Xpr1 | NNNCTCACTAGATAGCCCTG | NGG | 2 | 0.35 | Tier II |
14 | NC_000086.7 | X | 94260452 | - | Klhl15 | NNNCTCATCATATAGCCCAG | NGG | 2 | 0.25 | Tier II |
15 | NC_000068.7 | 2 | 155492944 | + | Ggt7 | NNNCTCACCATCAAGCCCTG | NGG | 2 | 0.1822 | Tier II |
16 | NC_000070.6 | 4 | 123923323 | + | Rragc | NNNCTCACGATATAGCCCTG | NAG | 2 | 0.1605 | Tier II |
17 | NC_000067.6 | 1 | 171243169 | - | Ndufs2 | NNNCTCACCATATAGCCATG | NAG | 2 | 0.1396 | Tier II |
18 | NC_000068.7 | 2 | 70212940 | + | Myo3b | NNNCTGACCATCTAGCCCTG | NGG | 2 | 0.1316 | Tier II |
19 | NC_000083.6 | 17 | 67774897 | - | Lama1 | NNNGTCACCATCTAGCCCTG | NGG | 2 | 0.1316 | Tier II |
20 | NC_000068.7 | 2 | 165610487 | - | Eya2 | NNNCTCACCATAGAGCACTG | NGG | 2 | 0.1217 | Tier II |
21 | NC_000083.6 | 17 | 26759589 | - | Crebrf | NNNCTCTCCATCTAGCCCTG | NGG | 2 | 0.1151 | Tier II |
22 | NC_000073.6 | 7 | 47350362 | - | Mrgpra1 | NNNCTCACCATGTAGCCCTG | NCG | 2 | 0.0774 | Tier II |
23 | NC_000082.6 | 16 | 17702116 | + | Med15 | NNNCTCACCATATAGCCTTG | NCG | 2 | 0.0689 | Tier II |
24 | NC_000086.7 | X | 147417785 | - | Il13ra2 | NNNCTCACCATGTAGCCCTG | NGA | 2 | 0.0502 | Tier II |
25 | NC_000070.6 | 4 | 44853759 | + | Zcchc7 | NNNCTCACTATATAGCCCTG | NTG | 2 | 0.0341 | Tier II |
26 | NC_000072.6 | 6 | 145003036 | + | Bcat1 | NNNCTCACTATATAGCCCTG | NTG | 2 | 0.0341 | Tier II |
27 | NC_000073.6 | 7 | 116274122 | - | Plekha7 | NNNCTCACTATATAGCCCTG | NTG | 2 | 0.0341 | Tier II |
28 | NC_000067.6 | 1 | 86241835 | + | Armc9 | NNNCTCACTATATAGCCCTG | NGC | 2 | 0.0194 | Tier II |
29 | NC_000069.6 | 3 | 132802640 | + | Tbck | NNNCTCACTATATAGCCCTG | NGC | 2 | 0.0194 | Tier II |
30 | NC_000073.6 | 7 | 99464675 | + | Klhl35 | NNNCTCACTATATAGCCCTG | NGC | 2 | 0.0194 | Tier II |
31 | NC_000070.6 | 4 | 132112204 | + | Oprd1 | NNNCTCACCATGTAGCCCTG | NGC | 2 | 0.016 | Tier II |
32 | NC_000075.6 | 9 | 64717839 | - | Rab11a | NNNCTCACTATATAGCCCTG | NGT | 2 | 0.0141 | Tier II |
33 | NC_000082.6 | 16 | 4316878 | + | Adcy9 | NNNCTCACTATATAGCCCTG | NGT | 2 | 0.0141 | Tier II |
34 | NC_000077.6 | 11 | 80162739 | - | Adap2 | NNNCTCACCATACAGCCCTG | NGT | 2 | 0.0127 | Tier II |
35 | NC_000076.6 | 10 | 94906017 | - | Plxnc1 | NNNCACACCATATAGCCCTG | NGC | 2 | 0.0111 | Tier II |
36 | NC_000084.6 | 18 | 32102600 | - | Iws1 | NNNCTCACCATATAGCCTTG | NGT | 2 | 0.0104 | Tier II |
37 | NC_000086.7 | X | 102540355 | + | Phka1 | NNNCTCAGCATATAGCCCTG | NGT | 2 | 0.0104 | Tier II |
38 | NC_000073.6 | 7 | 16280204 | - | Ccdc9 | NNNCTCACCATCTAGCCCTG | NTG | 2 | 0.0103 | Tier II |
39 | NC_000073.6 | 7 | 101206174 | + | Fchsd2 | NNNCTCACCATATAGTCCTG | NGT | 2 | 0.005 | Tier II |
40 | NC_000070.6 | 4 | 119335783 | + | Ccdc30 | NNNCTCACCATATAGCCCTG | NCT | 2 | 0.0 | Tier II |
41 | NC_000074.6 | 8 | 61873964 | + | Palld | NNNCTCACCATATAGCCCTG | NCT | 2 | 0.0 | Tier II |
42 | NC_000074.6 | 8 | 104370703 | + | Cmtm4 | NNNCTCACCATATAGCCCTG | NCT | 2 | 0.0 | Tier II |
43 | NC_000075.6 | 9 | 45218368 | - | BC049352 | NNNCTCACCATATAGCCCTG | NCT | 2 | 0.0 | Tier II |
44 | NC_000076.6 | 10 | 126877162 | + | Atp23 | NNNCTCACCATATAGCCCTG | NAT | 2 | 0.0 | Tier II |
45 | NC_000076.6 | 10 | 128103401 | - | Baz2a | NNNCTCACCATATAGCCCTG | NCA | 2 | 0.0 | Tier II |
46 | NC_000079.6 | 13 | 58595741 | - | Slc28a3 | NNNCTCACCATATAGCCCTG | NCT | 2 | 0.0 | Tier II |
47 | NC_000083.6 | 17 | 27064973 | - | Itpr3 | NNNCTCACCATATAGCCCTG | NCT | 2 | 0.0 | Tier II |
48 | NC_000073.6 | 7 | 81531118 | - | 2900076A07Rik | NNNCTCACTATGTAGCCCTG | NGG | 2 | 0.6319 | Tier III |
49 | NC_000080.6 | 14 | 25393419 | + | Zmiz1os1 | NNNCTGACCATATAACCCTG | NGG | 2 | 0.4706 | Tier III |
50 | NC_000085.6 | 19 | 10785432 | + | A430093F15Rik | NNNCTCTCCATCTAGCCCTG | NGG | 2 | 0.1151 | Tier III |
51 | NC_000067.6 | 1 | 31032304 | + | Gm28644 | NNNCTCACTATATAGCCCTG | NGT | 2 | 0.0141 | Tier III |
52 | NC_000067.6 | 1 | 31032304 | + | Gm29669 | NNNCTCACTATATAGCCCTG | NGT | 2 | 0.0141 | Tier III |
53 | NC_000077.6 | 11 | 29634924 | - | Gm12092 | NNNCTCACTATATAGCCCTG | NGT | 2 | 0.0141 | Tier III |
54 | NC_000086.7 | X | 140528407 | + | Gm39538 | NNNCTCACCATGTAGCCCTG | NGT | 2 | 0.0116 | Tier III |
55 | NC_000067.6 | 1 | 180547396 | - | Gm31986 | NNNCTCACCATATAGCCCTG | NCT | 2 | 0.0 | Tier III |
56 | NC_000081.6 | 15 | 54961887 | - | Gm26684 | NNNCTCACCATATAGCCCTG | NCT | 2 | 0.0 | Tier III |