Construct: sgRNA BRDN0001149433
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCATTCTAGATGCGTCCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ITPKC (80271)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77652
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
40717498 |
- |
ITPKC |
NNNATTCTAGATGCGTCCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
37987802 |
+ |
SHB |
NNNATTCTAGATGCGTCAGA |
NGG |
2 |
0.5048 |
Tier II |
| 3 |
NC_000002.12 |
2 |
66442762 |
+ |
MEIS1 |
NNNATTCTGGATGCGTCTGG |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000001.11 |
1 |
15524782 |
- |
CASP9 |
NNNCTTCTAGATGCGTCCCG |
NGG |
2 |
0.1582 |
Tier II |
| 5 |
NC_000010.11 |
10 |
77576100 |
- |
KCNMA1 |
NNNAATCTAGATGCGTCCGG |
NTG |
2 |
0.0195 |
Tier II |
| 6 |
NC_000019.10 |
19 |
2688705 |
+ |
GNG7 |
NNNATTCTAGATGGGTCCTG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000011.10 |
11 |
87696695 |
+ |
LOC107984361 |
NNNATTCTAGATGCTTCCAG |
NGG |
2 |
0.102 |
Tier III |
| 8 |
NC_000003.12 |
3 |
28577836 |
+ |
LINC00693 |
NNNATTCTAGATGCGACCGG |
NGT |
2 |
0.0147 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
79729743 |
+ |
Ppp1r1c |
NNNATTATAGATGCATCCGG |
NGG |
2 |
0.7059 |
Tier II |
Other clones with same target sequence:
(none)
