Construct: sgRNA BRDN0001149437
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGATTATGTGGCAACCCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDKL2 (8999)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77587
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
75607233 |
- |
CDKL2 |
NNNATTATGTGGCAACCCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
96205133 |
- |
MAML2 |
NNNATTATGTGGCAACCATA |
NGG |
2 |
0.359 |
Tier II |
| 3 |
NC_000001.11 |
1 |
49543693 |
- |
AGBL4 |
NNNAATATGTGGCAACCTGA |
NGG |
2 |
0.3214 |
Tier II |
| 4 |
NT_187386.1 |
22 |
4932 |
+ |
LOC124905330 |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier II |
| 5 |
NC_000002.12 |
2 |
178709376 |
- |
TTN |
NNNATTATGTGGCTTCCCGA |
NGG |
2 |
0.1067 |
Tier II |
| 6 |
NC_000010.11 |
10 |
69277061 |
+ |
HK1 |
NNNATTATGTGGCAAGCTGA |
NGG |
2 |
0.0989 |
Tier II |
| 7 |
NC_000012.12 |
12 |
45563524 |
- |
LOC105369743 |
NNNATTATATGGCAACCCTA |
NGG |
2 |
0.4286 |
Tier III |
| 8 |
NC_000022.11 |
22 |
18854576 |
- |
BCRP7 |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier III |
| 9 |
NC_000022.11 |
22 |
18854576 |
- |
POM121L15P |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier III |
| 10 |
NC_000022.11 |
22 |
21119994 |
+ |
BCRP2 |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier III |
| 11 |
NC_000022.11 |
22 |
21119994 |
+ |
POM121L7P |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier III |
| 12 |
NC_000022.11 |
22 |
21290522 |
- |
POM121L8P |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier III |
| 13 |
NC_000022.11 |
22 |
21290522 |
- |
BCRP6 |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier III |
| 14 |
NC_000022.11 |
22 |
22636389 |
+ |
BCRP4 |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier III |
| 15 |
NC_000022.11 |
22 |
22636389 |
+ |
IGL |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier III |
| 16 |
NC_000022.11 |
22 |
22636389 |
+ |
POM121L1P |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier III |
| 17 |
NC_000022.11 |
22 |
24651068 |
+ |
BCRP3 |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier III |
| 18 |
NC_000022.11 |
22 |
24651068 |
+ |
POM121L10P |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier III |
| 19 |
NT_187386.1 |
22 |
4932 |
+ |
LOC102724151 |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier III |
| 20 |
NT_187386.1 |
22 |
4932 |
+ |
LOC102724751 |
NNNTTTATGTGGCCACCCGA |
NGG |
2 |
0.1364 |
Tier III |
| 21 |
NC_000002.12 |
2 |
178709376 |
- |
LOC124906100 |
NNNATTATGTGGCTTCCCGA |
NGG |
2 |
0.1067 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
128759757 |
+ |
Mertk |
NNNATTATTTGGCAACCCAA |
NGG |
2 |
0.381 |
Tier II |
| 2 |
NC_000072.6 |
6 |
12410772 |
+ |
Thsd7a |
NNNATTATGTGTCAACCCTA |
NGG |
2 |
0.2564 |
Tier II |
| 3 |
NC_000069.6 |
3 |
88534562 |
- |
Mex3a |
NNNATTATGTGCCACCCCGA |
NGG |
2 |
0.1444 |
Tier II |
| 4 |
NC_000076.6 |
10 |
67095569 |
+ |
Reep3 |
NNNATTATGTGGAAACCCGA |
NGA |
2 |
0.0267 |
Tier II |
| 5 |
NC_000078.6 |
12 |
50474268 |
- |
Prkd1 |
NNNATTATGTGGCAACCCAA |
NGC |
2 |
0.0159 |
Tier II |
Other clones with same target sequence:
(none)
